| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| J A Affholter, C L Hsieh, U Francke, R A Rot. Insulin-degrading enzyme: stable expression of the human complementary DNA, characterization of its protein product, and chromosomal mapping of the human and mouse genes. Molecular endocrinology (Baltimore, Md.). vol 4. issue 8. 1991-04-09. PMID:2293021. |
overexpression of ide using this system should allow for a functional test of the role of ide in insulin action. |
1991-04-09 |
2023-08-11 |
mouse |
| W L Kuo, B D Gehm, M R Rosne. Cloning and expression of the cDNA for a Drosophila insulin-degrading enzyme. Molecular endocrinology (Baltimore, Md.). vol 4. issue 10. 1991-03-15. PMID:2126597. |
indirect studies based upon inhibitors, degradation products, and microinjected antibodies have suggested that the ide can initiate cellular insulin degradation in mammalian cells. |
1991-03-15 |
2023-08-11 |
human |
| J A Affholter, M A Cascieri, M L Bayne, J Brange, M Casaretto, R A Rot. Identification of residues in the insulin molecule important for binding to insulin-degrading enzyme. Biochemistry. vol 29. issue 33. 1991-02-25. PMID:2271531. |
insulin-degrading enzyme (ide) hydrolyzes insulin at a limited number of sites. |
1991-02-25 |
2023-08-11 |
Not clear |
| J A Affholter, M A Cascieri, M L Bayne, J Brange, M Casaretto, R A Rot. Identification of residues in the insulin molecule important for binding to insulin-degrading enzyme. Biochemistry. vol 29. issue 33. 1991-02-25. PMID:2271531. |
although the positions of these cleavages are known, the residues of insulin important in its binding to ide have not been defined. |
1991-02-25 |
2023-08-11 |
Not clear |
| J A Affholter, M A Cascieri, M L Bayne, J Brange, M Casaretto, R A Rot. Identification of residues in the insulin molecule important for binding to insulin-degrading enzyme. Biochemistry. vol 29. issue 33. 1991-02-25. PMID:2271531. |
to this end, we have studied the binding of a variety of insulin analogues to the protease in a solid-phase binding assay using immunoimmobilized ide. |
1991-02-25 |
2023-08-11 |
Not clear |
| J A Affholter, M A Cascieri, M L Bayne, J Brange, M Casaretto, R A Rot. Identification of residues in the insulin molecule important for binding to insulin-degrading enzyme. Biochemistry. vol 29. issue 33. 1991-02-25. PMID:2271531. |
since ide binds insulin with 600-fold greater affinity than it does insulin-like growth factor i (25 nm and approximately 16,000 nm, respectively), the first set of analogues studied were hybrid molecules of insulin and igf i. igf i mutants [insb1-17,17-70]igf i, [tyr55,gln56]igf i, and [phe23,phe24,tyr25]igf i have been synthesized and share the property of having insulin-like amino acids at positions corresponding to primary sites of cleavage of insulin by ide. |
1991-02-25 |
2023-08-11 |
Not clear |
| J A Affholter, M A Cascieri, M L Bayne, J Brange, M Casaretto, R A Rot. Identification of residues in the insulin molecule important for binding to insulin-degrading enzyme. Biochemistry. vol 29. issue 33. 1991-02-25. PMID:2271531. |
insulin mutants [b25-asp]insulin and [b25-his]insulin display 16- and 20-fold decreases in ide affinity versus wild-type insulin. |
1991-02-25 |
2023-08-11 |
Not clear |
| H Akiyama, K Yokono, K Shii, W Ogawa, H Taniguchi, S Baba, M Kasug. Natural regulatory mechanisms of insulin degradation by insulin degrading enzyme. Biochemical and biophysical research communications. vol 170. issue 3. 1990-09-27. PMID:2202301. |
insulin-degrading enzyme (ide) accounts for most of the insulin degrading activity in extracts of several tissues and plays an important role in the intracellular degradation of insulin. |
1990-09-27 |
2023-08-11 |
rat |
| H Akiyama, K Yokono, K Shii, W Ogawa, H Taniguchi, S Baba, M Kasug. Natural regulatory mechanisms of insulin degradation by insulin degrading enzyme. Biochemical and biophysical research communications. vol 170. issue 3. 1990-09-27. PMID:2202301. |
the ratio of insulin degrading activity to ide concentration was roughly the same in liver, brain and muscle, however, twice as high in kidney as compared with other tissues. |
1990-09-27 |
2023-08-11 |
rat |
| H Akiyama, K Yokono, K Shii, W Ogawa, H Taniguchi, S Baba, M Kasug. Natural regulatory mechanisms of insulin degradation by insulin degrading enzyme. Biochemical and biophysical research communications. vol 170. issue 3. 1990-09-27. PMID:2202301. |
these results suggest that ide degrades insulin in the initial step of cleavage and that there are some mechanisms to regulate insulin degrading activity by ide in the tissues. |
1990-09-27 |
2023-08-11 |
rat |
| K Yonezaw. [Biochemical characteristics of an initial degradation product of insulin by insulin-degrading enzyme]. Nihon Naibunpi Gakkai zasshi. vol 64. issue 11. 1989-03-21. PMID:2851464. |
we previously reported on the detection and hplc separation of the initial degradation product (peak vi) of native insulin from the reaction of monocomponent porcine insulin with affinity-purified pig skeletal muscle insulin-degrading enzyme (ide). |
1989-03-21 |
2023-08-11 |
rat |
| K Yonezaw. [Biochemical characteristics of an initial degradation product of insulin by insulin-degrading enzyme]. Nihon Naibunpi Gakkai zasshi. vol 64. issue 11. 1989-03-21. PMID:2851464. |
on the other hand, a cross-linking study suggested that peak vi preserves almost the same affinity to ide as insulin. |
1989-03-21 |
2023-08-11 |
rat |
| K Yonezaw. [Biochemical characteristics of an initial degradation product of insulin by insulin-degrading enzyme]. Nihon Naibunpi Gakkai zasshi. vol 64. issue 11. 1989-03-21. PMID:2851464. |
these findings may indicate the possibility that pig skeletal muscle ide cleaves peptide bonds within a-chain at an early step of degradation of native porcine insulin and generates peak vi, which is the next substrate to insulin for ide and keeps reduced insulin-like biological potencies, and then peak vi is converted into several relatively low molecular weight products. |
1989-03-21 |
2023-08-11 |
rat |
| W C Duckwort. Insulin degradation: mechanisms, products, and significance. Endocrine reviews. vol 9. issue 3. 1989-02-23. PMID:3061785. |
the importance of ip (ide) in cellular insulin degradation has been established and the importance of lysosomal degradation questioned. |
1989-02-23 |
2023-08-11 |
Not clear |
| J V Garcia, M P Stoppelli, S J Decker, M R Rosne. An insulin epidermal growth factor-binding protein from Drosophila has insulin-degrading activity. The Journal of cell biology. vol 108. issue 1. 1989-02-13. PMID:2492023. |
we have recently described the purification and characterization of an insulin-degrading enzyme (ide) from drosophila melanogaster that can cleave porcine insulin, is highly conserved through evolution and is developmentally regulated. |
1989-02-13 |
2023-08-11 |
human |
| J V Garcia, M P Stoppelli, S J Decker, M R Rosne. An insulin epidermal growth factor-binding protein from Drosophila has insulin-degrading activity. The Journal of cell biology. vol 108. issue 1. 1989-02-13. PMID:2492023. |
we now report that the ide is, in fact, an insulin egf-binding protein (dp100) that we had isolated previously from drosophila using an antihuman egf receptor antiserum. |
1989-02-13 |
2023-08-11 |
human |
| J V Garcia, M P Stoppelli, S J Decker, M R Rosne. An insulin epidermal growth factor-binding protein from Drosophila has insulin-degrading activity. The Journal of cell biology. vol 108. issue 1. 1989-02-13. PMID:2492023. |
(a) dp100, identified by its ability to cross-link to labeled insulin, egf, and transforming growth factor-alpha (tgf-alpha), and to be immunoprecipitated by anti-egf receptor antisera, copurifies with the ide activity. |
1989-02-13 |
2023-08-11 |
human |
| J V Garcia, M P Stoppelli, S J Decker, M R Rosne. An insulin epidermal growth factor-binding protein from Drosophila has insulin-degrading activity. The Journal of cell biology. vol 108. issue 1. 1989-02-13. PMID:2492023. |
thus, the purified ide can be affinity labeled with either 125i-insulin, 125i-egf, or 125i-tgf-alpha, and this labeling is specifically inhibited with unlabeled insulin, egf, and the insulin b chain. |
1989-02-13 |
2023-08-11 |
human |
| J V Garcia, M P Stoppelli, S J Decker, M R Rosne. An insulin epidermal growth factor-binding protein from Drosophila has insulin-degrading activity. The Journal of cell biology. vol 108. issue 1. 1989-02-13. PMID:2492023. |
(e) egf, which competes with insulin for binding to dp100, also inhibits the degradation of insulin by the purified ide. |
1989-02-13 |
2023-08-11 |
human |
| K Yonezawa, K Yokono, K Shii, J Hari, S Yaso, T Sakamoto, Y Kawase, H Akiyama, S Taketomi, S Bab. Biological properties of an initial degradation product of insulin by insulin-degrading enzyme. Endocrinology. vol 124. issue 1. 1989-02-07. PMID:2642422. |
we previously reported on the degradation of monocomponent porcine insulin by affinity-purified pig skeletal muscle insulin-degrading enzyme (ide) and on the detection and hplc separation of the initial degradation product (peak vi). |
1989-02-07 |
2023-08-11 |
human |