| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| M Kobayashi, M Iwasaki, N Watanabe, O Ishibashi, Y Takata, T Haruta, T Sasaoka, Y Shigeta, K Inouy. Metabolism of a mutant insulin by a receptor-mediated process and an insulin degrading enzyme. Diabetes research and clinical practice. vol 2. issue 5. 1987-01-08. PMID:3536368. |
these results suggest that the decreased clearance of insulin is due to the decreased receptor binding and the decreased receptor-mediated degradation, but is not due to the decreased degradation by ide. |
1987-01-08 |
2023-08-11 |
rat |
| K Yonezawa, K Yokono, S Yaso, J Hari, K Amano, Y Kawase, T Sakamoto, K Shii, Y Imamura, S Bab. Degradation of insulin by insulin-degrading enzyme and biological characteristics of its fragments. Endocrinology. vol 118. issue 5. 1986-06-02. PMID:3516656. |
in the present study, we have investigated the detection and the hplc separation of degradation products of native insulin from the reaction of monocomponent porcine insulin with affinity-purified pig ide. |
1986-06-02 |
2023-08-11 |
rat |
| K Yonezawa, K Yokono, S Yaso, J Hari, K Amano, Y Kawase, T Sakamoto, K Shii, Y Imamura, S Bab. Degradation of insulin by insulin-degrading enzyme and biological characteristics of its fragments. Endocrinology. vol 118. issue 5. 1986-06-02. PMID:3516656. |
insulin was degraded by ide in a time- and dose-dependent manner. |
1986-06-02 |
2023-08-11 |
rat |
| K Yonezawa, K Yokono, S Yaso, J Hari, K Amano, Y Kawase, T Sakamoto, K Shii, Y Imamura, S Bab. Degradation of insulin by insulin-degrading enzyme and biological characteristics of its fragments. Endocrinology. vol 118. issue 5. 1986-06-02. PMID:3516656. |
among seven peaks representing degradation products, peak vi appeared most rapidly at 30 sec of incubation, increased until 10 min, and then decreased after 15 min of incubation; and six degradation products other than peak vi were not detected within 15 min of incubation, suggesting that peak vi was an initial degradation product of insulin produced by ide and converted into relatively low molecular weight products as incubation time increased. |
1986-06-02 |
2023-08-11 |
rat |
| K Yonezawa, K Yokono, S Yaso, J Hari, K Amano, Y Kawase, T Sakamoto, K Shii, Y Imamura, S Bab. Degradation of insulin by insulin-degrading enzyme and biological characteristics of its fragments. Endocrinology. vol 118. issue 5. 1986-06-02. PMID:3516656. |
since some of these peptides have insulin-like properties, amino acid analysis of these products may enable us to identify not only the splitting position of insulin by ide but also the site of the hormone for receptor binding. |
1986-06-02 |
2023-08-11 |
rat |
| K Shii, K Yokono, Y Imamura, J Hari, S Bab. [Insulin degrading system on isolated rat hepatocytes; enzymatic and immunologic characteristics of insulin-degrading activities]. Nihon Naibunpi Gakkai zasshi. vol 60. issue 1. 1984-09-10. PMID:6378676. |
in order to characterize the enzymatic properties of insulin-degrading activity by isolated hepatocytes, the effects of various compounds and anti-insulin-degrading enzyme (ide) rabbit serum on insulin degradation were examined. |
1984-09-10 |
2023-08-12 |
rat |
| K Shii, K Yokono, Y Imamura, J Hari, S Bab. [Insulin degrading system on isolated rat hepatocytes; enzymatic and immunologic characteristics of insulin-degrading activities]. Nihon Naibunpi Gakkai zasshi. vol 60. issue 1. 1984-09-10. PMID:6378676. |
therefore, it would seem that ide plays an important role in insulin metabolism by isolated rat hepatocytes rather than lysosomes. |
1984-09-10 |
2023-08-12 |
rat |
| I D Goldfine, J A Williams, A C Bailey, K Y Wong, Y Iwamoto, K Yokono, S Baba, R A Rot. Degradation of insulin by isolated mouse pancreatic acini. Evidence for cell surface protease activity. Diabetes. vol 33. issue 1. 1984-02-21. PMID:6360769. |
several lines of evidence suggested that insulin was being degraded by the neutral thiol protease, insulin degrading enzyme (ide). |
1984-02-21 |
2023-08-12 |
mouse |
| K Yokono, Y Imamura, K Shii, N Mizuno, H Sakai, S Bab. Immunochemical studies on the insulin-degrading enzyme from pig and rat skeletal muscle. Diabetes. vol 29. issue 10. 1981-02-26. PMID:6777221. |
insulin-degrading enzyme (ide), which proteolytically degraded insulin with a high degree of specificity, was purified from pig skeletal muscle by ammonium sulfate precipitation, chromatography on bio-gel p-200 and deae-cellulose, and finally rechromatography on sephadex g-200 (rechromatography fraction). |
1981-02-26 |
2023-08-12 |
rat |