All Relations between blot and insulin

Publication Sentence Publish Date Extraction Date Species
Jing Sun, Kongyan Wu, Pan Wang, Yubin Wang, Dan Wang, Wenting Zhao, Yuanyuan Zhao, Chunhong Zhang, Xiaoyan Zha. Dietary Tomato Pectin Attenuates Hepatic Insulin Resistance and Inflammation in High-Fat-Diet Mice by Regulating the PI3K/AKT Pathway. Foods (Basel, Switzerland). vol 13. issue 3. 2024-02-10. PMID:38338579. the underlying mechanisms involved in the inflammation, oxidative stress and insulin signaling in the liver were also investigated by rt-qpcr and western blot, which indicated that tp ameliorated hepatic insulin resistance by regulating the pi3k/akt/gsk-3β pathway, increasing the expression of glut4, decreasing the expression of peck and g6p as well as restoring antioxidant activities and suppressing the inflammation statues in hfd-fed mice. 2024-02-10 2024-02-12 mouse
Qing Liu, Ying Han, Meng Zhang, Peng Yang, Yan Xiang, Min Chen, Fei Xu, Xiaochan Zhou, Dan Zheng, Juan Qi. IGF1R stimulates autophagy, enhances viability, and promotes insulin secretion in pancreatic β cells in gestational diabetes mellitus by upregulating ATG7. Reproductive biology. vol 24. issue 1. 2024-01-23. PMID:38262267. additionally, in mouse pancreatic β cells, igf1r, atg7, beclin-1, and lc3-ii/lc3-i levels, cell viability/apoptosis, and insulin level were assessed by western blot, cck-8, flow cytometry, and elisa. 2024-01-23 2024-01-26 mouse
Soon-Young Kim, Seung-Yoon Park, Jung-Eun Ki. GULP1 deficiency reduces adipogenesis and glucose uptake via downregulation of PPAR signaling and disturbing of insulin/ERK signaling in 3T3-L1 cells. Journal of cellular physiology. 2024-01-12. PMID:38214103. glucose uptake was monitored via insulin stimulation in shgulp1 and u6 cells using a 2-nbdg glucose uptake assay, and the insulin signaling pathway was investigated by western blot analysis. 2024-01-12 2024-01-14 Not clear
Gonçalo M Melo, Adriana M Capucho, Joana F Sacramento, José Ponce-de-Leão, Marcos V Fernandes, Inês F Almeida, Fátima O Martins, Silvia V Cond. Overnutrition during Pregnancy and Lactation Induces Gender-Dependent Dysmetabolism in the Offspring Accompanied by Heightened Stress and Anxiety. Nutrients. vol 16. issue 1. 2024-01-11. PMID:38201896. the offspring were analyzed at postnatal days 21 and 28 and at 2 months old (pd21, pd28, and pd60) for their metabolic profiles (weight, fasting glycemia insulin sensitivity, and glucose tolerance) and euthanized for brain collection to evaluate metabolism and inflammation in the hypothalamus, hippocampus, and prefrontal cortex using western blot markers of synaptic dynamics. 2024-01-11 2024-01-13 rat
Mengqi Xiang, Xinmeng Yuan, Nianyun Zhang, Liumei Zhang, Yuting Liu, Jingjing Liu, Yaran Gao, Ye Xu, Wen Sun, Qiang Tang, Yuan Zhang, Jiao L. Effects of exercise, metformin, and combination treatments on type 2 diabetic mellitus-induced muscle atrophy in db/db mice: Crosstalk between autophagy and the proteasome. Journal of physiology and biochemistry. 2023-12-19. PMID:38112970. the levels of insulin signaling pathway proteins, ubiquitin proteasome, and autophagic lysosome-associated proteins were detected using western blot, the expression of murf1 and atrogin-1 was detected using immunohistochemical staining, and the degradation of autophagosomes was detected using double-labeled immunofluorescence. 2023-12-19 2023-12-23 mouse
Yue Jiang, Li-Ke Yan, Ying Wang, Jun-Feng Ding, Zhong-Hua Xu, Can Cui, Jun T. [Mechanism of Gegen Qinlian Decoction in improving glucose metabolism in vitro and in vivo by alleviating hepatic endoplasmic reticulum stress]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 20. 2023-12-19. PMID:38114149. in addition, the phosphorylation levels of c-jun n-terminal kinases(jnks), which were involved in both the ers and insulin signaling pathways, were measured by western blot. 2023-12-19 2023-12-23 rat
Zhen Yu, Junyong Han, Lisha Li, Qiufeng Zhang, Ayun Chen, Jinyan Chen, Kun Wang, Jingjun Jin, Huangyuan Li, Gang Che. Chronic triclosan exposure induce impaired glucose tolerance by altering the gut microbiota. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2023-12-05. PMID:38052405. western blot and immunostaining results further confirmed that elevated bcaas stimulated mtor, a nutrient-sensing complex, and following irs-1 serine phosphorylation, resulted in insulin resistance and glucose intolerance. 2023-12-05 2023-12-10 human
Juliane Weiner, Sebastian Dommel, Claudia Gebhardt, Martha Hanschkow, Yulia Popkova, Kerstin Krause, Nora Klöting, Matthias Blüher, Jürgen Schiller, John T Heike. Differential expression of immunoregulatory cytokines in adipose tissue and liver in response to high fat and high sugar diets in female mice. Frontiers in nutrition. vol 10. 2023-12-01. PMID:38024380. mice were extensively phenotyped, including glucose and insulin tolerance tests, adipose and liver gene and protein expression analysis by qpcr and western blot, tissue lipid analyses, as well as histological analyses. 2023-12-01 2023-12-07 mouse
Juliane Weiner, Sebastian Dommel, Claudia Gebhardt, Martha Hanschkow, Yulia Popkova, Kerstin Krause, Nora Klöting, Matthias Blüher, Jürgen Schiller, John T Heike. Differential expression of immunoregulatory cytokines in adipose tissue and liver in response to high fat and high sugar diets in female mice. Frontiers in nutrition. vol 10. 2023-12-01. PMID:38024380. mice were extensively phenotyped, including glucose and insulin tolerance tests, adipose and liver gene and protein expression analysis by qpcr and western blot, tissue lipid analyses, as well as histological analyses. 2023-11-29 2023-12-07 mouse
Xiao-Qi Liu, Tian Hu, Guang-Liang Wu, Li-Jun Qiao, Ye-Feng Cai, Qi Wang, Shi-Jie Zhan. Tanshinone IIA, the key compound in Salvia miltiorrhiza, improves cognitive impairment by upregulating Aβ-degrading enzymes in APP/PS1 mice. International journal of biological macromolecules. 2023-11-09. PMID:37944734. western blot results confirmed that the expressions of insulin degrading enzymes (ide) and neprilysin (nep) were significantly increased after tan iia treatment, which demonstrated that tan iia improved ad by increasing aβ-degrading enzymes. 2023-11-09 2023-11-20 mouse
Caroline N Rivera, Rachel M Watne, Andrew J Wommack, Roger A Vaugha. The effect of insulin resistance on extracellular BCAA accumulation and SLC25A44 expression in a myotube model of skeletal muscle insulin resistance. Amino acids. 2023-09-23. PMID:37740788. insulin sensitivity and slc25a44 expression were assessed via western blot. 2023-09-23 2023-10-07 Not clear
Chan Yang, Hanyu Liu, Ziyan Xie, Qiangfei Yang, Lian Du, Chunguang Xi. The protective role of shenqi compound in type 2 diabetes: A comprehensive investigation of pancreatic β-cell function and mass. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 166. 2023-08-12. PMID:37572639. the results demonstrate multiple beneficial effects of sqc, including significant control of blood glucose levels (p < 0.05), inhibition of insulin resistance (measured by western blot), reduction of hyperinsulinemia (p < 0.05), attenuation of oxidative stress (p < 0.05), suppression of inflammation (p < 0.05), protection against islet hypertrophy and beta cell proliferation (evaluated through pathological staining), and inhibition of β-cell apoptosis and senescence (also assessed through pathological staining). 2023-08-12 2023-08-16 rat
Minchuan Zhou, Jingqing Huang, Jingwen Zhou, Cuiting Zhi, Yan Bai, Qishi Che, Hua Cao, Jiao Guo, Zhengquan S. Anti-Obesity Effect and Mechanism of Chitooligosaccharides Were Revealed Based on Lipidomics in Diet-Induced Obese Mice. Molecules (Basel, Switzerland). vol 28. issue 14. 2023-07-29. PMID:37513467. the western blot assay demonstrated that cosm intervention can improve insulin resistance, inhibit de novo synthesis, and promote thermogenesis and β-oxidation in mitochondria by the ampk pathway, thereby alleviating high-fat diet-induced obesity. 2023-07-29 2023-08-14 mouse
Ranran Sun, Liwei Jiang, Wenlong Chen, Yuanhao Xu, Xin Yi, Guohua Zhon. Azadirachtin exposure inhibit ovary development of Spodoptera litura (Lepidoptera: Noctuidae) by altering lipids metabolism event and inhibiting insulin signaling pathways. Ecotoxicology and environmental safety. vol 262. 2023-06-25. PMID:37356396. besides, following the abnormal lipids metabolism, western blot analysis suggested that aza induce insulin resistance-like phenotypes by inhibiting insulin receptor substrates (irs) /pi3k/akt pathway, which might be responsible for the ovary abnormalities of s. litura. 2023-06-25 2023-08-14 Not clear
Jing Xu, Xiaoyu Fan, Mou Zhu, Zhenglin Zhao, Xiaodan Qi, Han Gao, Ying Liu, Yan Shi, Chunjing Zhan. Ginsenoside Rg3 Protects Mouse Islet β-Cells Injured by High Glucose. Indian journal of microbiology. vol 63. issue 2. 2023-06-16. PMID:37325021. the cultured mouse pancreatic islet min6 cells were divided into control group (nc), rg3 group (rg3, 50 μg/l), high glucose group (hg, 33 mmol/l), high glucose and rg3 group (hg + rg3), after 48 h of continuous culture, cck-8 was used to detect cell viability; mouse insulin enzyme-linked immunoassay kit to detect insulin release; atp content detection kit to detect atp; dcfh-da to detect intracellular reactive oxygen species (ros) levels; total glutathione (t-gsh)/oxidized glutathione (gssg) assay kit to detect the ratio of gsh/gssg; using the mitochondrial membrane channel pore (mptp) fluorescence detection kit in min6 cells and collect the intensity of green fluorescence; western blot to detect the expression of antioxidant proteins glutathione reductase (gr). 2023-06-16 2023-08-14 mouse
Hakan Soylu, Kayihan Karaco. The effects of hydroxytyrosol on Prdx6 and insulin expression in diabetic rat pancreases. Histochemistry and cell biology. 2023-05-23. PMID:37219732. insulin expression was determined by immunohistochemistry and prdx6 expression was determined by immunohistochemistry and western blot. 2023-05-23 2023-08-14 rat
Lihui Ni, Yuan Li, Haifeng Zhang, Yunxiao Ma, Yuli Song, Xudong Tang, Junwen Fan, Jin Yang Shi, Xinhua Cui, Hongyue Xu, Hong Zhou, Keshu Shen, Weiying Guo, Lu Y. The combination of insulin and linezolid ameliorates Staphylococcus aureus pneumonia in individuals with diabetes via the TLR2/MAPKs/NLRP3 pathway. International journal of biological macromolecules. 2023-05-09. PMID:37160172. in vitro, our drug sensitivity assay, bacterial growth curve and hemolytic tests showed that a combination of insulin and linezolid exerted good antibacterial and anti-α-hemolysin activity, cck8 experiment, glucose content and glycogen content determination showed that the combination of insulin and linezolid increased murine macrophage survival rate and reduced the extracellular glucose level of high glucose-treated mh-s cells and intracellular glycogen level, and western blot showed that the combination inhibited tlr2/mapks/nlrp3-related inflammatory pathways in mh-s cells. 2023-05-09 2023-08-14 mouse
Ana Knezovic, Marija Piknjac, Jelena Osmanovic Barilar, Ana Babic Perhoc, Davor Virag, Jan Homolak, Melita Salkovic-Petrisi. Association of Cognitive Deficit with Glutamate and Insulin Signaling in a Rat Model of Parkinson's Disease. Biomedicines. vol 11. issue 3. 2023-03-29. PMID:36979662. the expression of tyrosine hydroxylase (th) and proteins involved in insulin (insulin receptor - ir, phosphoinositide 3 kinase - pi3k, extracellular signal-regulated kinases-erk) and glutamate receptor (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptos-ampar, n-methyl-d-aspartate receptor - nmdar) signaling was assessed in the hippocampus (hpc), hypothalamus (hpt) and striatum (s) by immunofluorescence, western blot and enzyme-linked immunosorbent assay (elisa). 2023-03-29 2023-08-14 rat
Man Zhao, Qian Meng, Man Zhan. Urinary insulin signaling pathway related proteins may serve as potential biomarkers for monitoring diabetes mellitus without hypertension and hyperlipidemia. Medicine. vol 102. issue 5. 2023-02-07. PMID:36749274. western blot and enzyme-linked immunoassay were performed to verify the expression of insulin signaling pathway related proteins in the urine of diabetic patients. 2023-02-07 2023-08-14 Not clear
Xueyao Yin, Zhiye Xu, Xinxin Zhang, Jiahua Wu, Weina L. Deficiency of lipopolysaccharide binding protein facilitates adipose browning, glucose uptake and oxygen consumption in mouse embryonic fibroblasts via activating PI3K/Akt/mTOR pathway and inhibiting autophagy. Cell cycle (Georgetown, Tex.). 2023-01-30. PMID:36710409. the expression levels of lbp, inflammatory markers , brown fat markers, lipid metabolism marker, autophagy markers, insulin signaling-related molecules , p-mtor, mtor, p-akt, akt, p-pi3k, and pi3k were quantified or determined by western blot, qrt-pcr, and immunofluorescence assay. 2023-01-30 2023-08-14 mouse