All Relations between blot and insulin

Publication Sentence Publish Date Extraction Date Species
Jingzhi Wang, Zhongyu Huang, Yiwen Li, Qian Li, Xi Li, Li Che. Electroacupuncture improves cognitive function in high-fat diet/streptozocin-induced type 2 diabetic mice by inhibiting autophagy-related ferroptosis. Experimental animals. 2024-12-08. PMID:39647901. hippocampal insulin resistance was determined by western blot analysis. 2024-12-08 2024-12-11 mouse
Bing Yang, Ruyan Yang, Xinyu Zhang, Wanjia Wang, Jianquan Ka. Hovenia dulcis (Guaizao) polysaccharide ameliorates hyperglycemia through multiple signaling pathways in rats with type 2 diabetes mellitus. International journal of biological macromolecules. 2024-12-05. PMID:39638196. real-time quantitative pcr and western blot analyses indicated that hdps-2a significantly enhanced the expression of insr, irs2, pi3k, akt, and glut4, suggesting that hdps-2a regulates insulin resistance and glycometabolism through the activation of the pi3k/akt signaling pathway. 2024-12-05 2024-12-08 human
F Frers, J Delarocque, K Feige, K Huber, T Warnke. Insulin signaling in insulin-dysregulated Icelandic horses. Domestic animal endocrinology. vol 86. 2024-11-04. PMID:39491260. western blot analysis of key proteins of the insulin signaling cascade revealed an enhanced phosphorylation of the insulin receptor (insr) under gluc po (p = 0.001) and ins iv stimulation (p = 0.017) within lt, but not in mt and at. 2024-11-04 2024-11-06 Not clear
S Melini, C Pirozzi, A Lama, F Comella, N Opallo, F Del Piano, E Di Napoli, M P Mollica, O Paciello, M C Ferrante, G Mattace Raso, R Mel. Co-Micronized Palmitoylethanolamide and Rutin Associated With Hydroxytyrosol Recover Diabesity-Induced Hepatic Dysfunction in Mice: In Vitro Insights Into the Synergistic Effect. Phytotherapy research : PTR. 2024-10-30. PMID:39474783. the formulation improved hfd-altered insulin sensitivity and hepatic glucose production and metabolism, as shown by glucose, insulin, pyruvate tolerance tests, western blot, and real-time pcr. 2024-10-30 2024-11-02 mouse
Xiangming Ma, Fei Tian, Jian Li, Zhenyu Wu, Liying Ca. In Vitro Simulated Ketogenic Diet Inhibits the Proliferation and Migration of Liver Cancer Cells by Reducing Insulin Production and Down-regulating FOXC2 Expression. The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology. vol 35. issue 9. 2024-09-30. PMID:39344752. western blot was utilized for detecting the expression of glycolysis-related proteins, seahorse xf96 for oxygen consumption rate (ocr) and extracellular acidification rate (ecar), and elisa for insulin content. 2024-09-30 2024-10-02 Not clear
Ansuman Sahoo, Prabir Kumar Das, Veeranki Venkata Dasu, Sanjukta Patr. Production of recombinant human insulin from a promising Pseudomonas fluorescens cell factory and its kinetic modeling. International journal of biological macromolecules. 2024-09-18. PMID:39293616. the validity of insulin and proinsulin fusion protein is assessed using western blot and quantified using elisa techniques. 2024-09-18 2024-09-21 human
Emily C Wyatt, Lindsey R VanDerStad, Norah E Cook, Macey R McGovern, Toheed Zaman, Pamela M Lundin, Roger A Vaugha. Valsartan Rescues Suppressed Mitochondrial Metabolism during Insulin Resistance in C2C12 Myotubes. Cell biochemistry and function. vol 42. issue 7. 2024-09-07. PMID:39243192. gene expression was assessed via qrt-pcr, and insulin sensitivity was assessed via western blot. 2024-09-07 2024-09-10 Not clear
Giulia Querio, Susanna Antoniotti, Renzo Levi, Bernd K Fleischmann, Maria Pia Gallo, Daniela Mala. Insulin-Activated Signaling Pathway and GLUT4 Membrane Translocation in hiPSC-Derived Cardiomyocytes. International journal of molecular sciences. vol 25. issue 15. 2024-08-10. PMID:39125765. western blot analysis on total cell lysates obtained from hipsc-cm showed increased phosphorylation of both akt and as160 following insulin treatment, but failed to highlight any changes in the expression dynamics of the glucose transporter glut4. 2024-08-10 2024-08-13 human
Hao Chen, Shi-Han Wang, Hong-Li Li, Xiao-Bo Zhou, Lin-Wei Zhou, Chang Chen, Toby Mansell, Boris Novakovic, Richard Saffery, Philip N Baker, Ting-Li Han, Hua Zhan. The attenuation of gut microbiota-derived short-chain fatty acids elevates lipid transportation through suppression of the intestinal HDAC3-H3K27ac-PPAR-γ axis in gestational diabetes mellitus. The Journal of nutritional biochemistry. 2024-07-26. PMID:39059479. western blot, elisa, and chromatin immunoprecipitation (chip) were utilized to elucidate how gut microbiota affect intestinal lipid transport and the insulin sensitivity of hepatic, adipose, and skeletal muscle tissues. 2024-07-26 2024-07-29 mouse
Eddie Tam, Khang Nguyen, Hye Kyoung Sung, Gary Sweene. MitoNEET preserves muscle insulin sensitivity during iron overload by regulating mitochondrial iron, reactive oxygen species and fission. The FEBS journal. 2024-06-30. PMID:38944692. in l6 control cells, io resulted in insulin resistance which could be prevented by miton as demonstrated by western blot of p-akt and akt biosensor cells. 2024-06-30 2024-07-02 Not clear
Yiyi Jin, Zhaoshui Shangguan, Jiao Pang, Yuwen Chen, Suijin Lin, Hekun Li. Pin1 Exacerbates Non-Alcoholic Fatty Liver Disease by Enhancing Its Activity through Binding to ACC1. International journal of molecular sciences. vol 25. issue 11. 2024-06-19. PMID:38892011. the effects of pin1 on lipid accumulation, hepatic fibrosis, and oxidative stress were evaluated by biochemical analysis, glucose and insulin tolerance tests, histological analysis, ihc, rt-qpcr and western blot assays. 2024-06-19 2024-06-21 mouse
Haining Du, Lili Cui, Xinyi Zhao, Ziteng Yu, Tianyue He, Boya Zhang, Xingpei Fan, Meimei Zhao, Ruijiao Zhu, Ziyi Zhang, Mengcong Li, Jiaxin Li, Yuri Oh, Ning G. Butylparaben induces glycolipid metabolic disorders in mice via disruption of gut microbiota and FXR signaling. Journal of hazardous materials. vol 474. 2024-06-08. PMID:38850927. western blot results further validated that butylparaben promoted hepatic lipogenesis, inflammation, gluconeogenesis, and insulin resistance through the inhibition of the farnesoid x receptor (fxr) pathway. 2024-06-08 2024-06-11 mouse
Piotr Zabielski, Monika Imierska, Kamila Roszczyc-Owsiejczuk, Mariusz Kuźmicki, Paweł Rogalski, Jarosław Daniluk, Agnieszka U Błachnio-Zabielsk. The Role of Acyl-CoA Synthetase 1 in Bioactive Lipid Accumulation and the Development of Hepatic Insulin Resistance. Nutrients. vol 16. issue 7. 2024-04-13. PMID:38613036. the protein expression and phosphorylation state of the insulin pathway was estimated by western blot. 2024-04-13 2024-04-15 mouse
Zhengzheng Ju, Fan Cui, Zheng Mao, Zhen Li, Xiayu Yi, Jingjing Zhou, Jinjin Cao, Xiaoqin Li, Zengkun Qia. miR-335-3p improves type II diabetes mellitus by IGF-1 regulating macrophage polarization. Open medicine (Warsaw, Poland). vol 19. issue 1. 2024-03-11. PMID:38463527. blood glucose (insulin tolerance tests, glucose tolerance tests) and body weight of the t2dm model were measured; macrophages from adipose tissue were isolated and cultured, and the number of macrophages was detected by f4/80 immunofluorescence assay; the real-time quantitative polymerase chain reaction (qpcr) assay and western blot assay were used to detect the mir-335-3p expression levels, insulin-like growth factor 1 (igf-1), m1-polarizing genes (inducible nitric oxide synthase [inos] and tnf-α) as well as m2-polarizing genes (il-10 and arg-1). 2024-03-11 2024-03-14 mouse
Adjoa Osei-Ntansah, Trinitee Oliver, Taylor Lofton, Claire Falzarano, Kiana Carr, Ruthe Huang, Andre Wilson, Ella Damaser, Guyton Harvey, Md Ahasanur Rahman, Stanley Andriss. Liver Androgen Receptor Knockout Improved High-fat Diet Induced Glucose Dysregulation in Female Mice But Not Male Mice. Journal of the Endocrine Society. vol 8. issue 4. 2024-03-01. PMID:38425436. additionally, we performed western blot and quantitative real-time pcr analysis on the livers of the mice to examine intermediates in the insulin signaling pathway. 2024-03-01 2024-03-03 mouse
Mara Patricia Chávez-Ortega, Julio Cesar Almanza-Pérez, Fausto Sánchez-Muñoz, Enrique Hong, Elihu Velázquez-Reyes, Rodrigo Romero-Nava, Santiago Villafaña-Rauda, Alfredo Pérez-Ontiveros, Gerardo Blancas-Flores, Fengyang Huan. Effect of Supplementation with Omega-3 Polyunsaturated Fatty Acids on Metabolic Modulators in Skeletal Muscle of Rats with an Obesogenic High-Fat Diet. Pharmaceuticals (Basel, Switzerland). vol 17. issue 2. 2024-02-24. PMID:38399437. insulin resistance was assessed, and gene and protein expression of metabolism modulators in skeletal muscle was also calculated using pcr-rt and western blot. 2024-02-24 2024-02-26 rat
Jing Sun, Kongyan Wu, Pan Wang, Yubin Wang, Dan Wang, Wenting Zhao, Yuanyuan Zhao, Chunhong Zhang, Xiaoyan Zha. Dietary Tomato Pectin Attenuates Hepatic Insulin Resistance and Inflammation in High-Fat-Diet Mice by Regulating the PI3K/AKT Pathway. Foods (Basel, Switzerland). vol 13. issue 3. 2024-02-10. PMID:38338579. the underlying mechanisms involved in the inflammation, oxidative stress and insulin signaling in the liver were also investigated by rt-qpcr and western blot, which indicated that tp ameliorated hepatic insulin resistance by regulating the pi3k/akt/gsk-3β pathway, increasing the expression of glut4, decreasing the expression of peck and g6p as well as restoring antioxidant activities and suppressing the inflammation statues in hfd-fed mice. 2024-02-10 2024-02-12 mouse
Qing Liu, Ying Han, Meng Zhang, Peng Yang, Yan Xiang, Min Chen, Fei Xu, Xiaochan Zhou, Dan Zheng, Juan Qi. IGF1R stimulates autophagy, enhances viability, and promotes insulin secretion in pancreatic β cells in gestational diabetes mellitus by upregulating ATG7. Reproductive biology. vol 24. issue 1. 2024-01-23. PMID:38262267. additionally, in mouse pancreatic β cells, igf1r, atg7, beclin-1, and lc3-ii/lc3-i levels, cell viability/apoptosis, and insulin level were assessed by western blot, cck-8, flow cytometry, and elisa. 2024-01-23 2024-01-26 mouse
Soon-Young Kim, Seung-Yoon Park, Jung-Eun Ki. GULP1 deficiency reduces adipogenesis and glucose uptake via downregulation of PPAR signaling and disturbing of insulin/ERK signaling in 3T3-L1 cells. Journal of cellular physiology. 2024-01-12. PMID:38214103. glucose uptake was monitored via insulin stimulation in shgulp1 and u6 cells using a 2-nbdg glucose uptake assay, and the insulin signaling pathway was investigated by western blot analysis. 2024-01-12 2024-01-14 Not clear
Gonçalo M Melo, Adriana M Capucho, Joana F Sacramento, José Ponce-de-Leão, Marcos V Fernandes, Inês F Almeida, Fátima O Martins, Silvia V Cond. Overnutrition during Pregnancy and Lactation Induces Gender-Dependent Dysmetabolism in the Offspring Accompanied by Heightened Stress and Anxiety. Nutrients. vol 16. issue 1. 2024-01-11. PMID:38201896. the offspring were analyzed at postnatal days 21 and 28 and at 2 months old (pd21, pd28, and pd60) for their metabolic profiles (weight, fasting glycemia insulin sensitivity, and glucose tolerance) and euthanized for brain collection to evaluate metabolism and inflammation in the hypothalamus, hippocampus, and prefrontal cortex using western blot markers of synaptic dynamics. 2024-01-11 2024-01-13 rat