All Relations between blot and prefrontal cortex

Publication Sentence Publish Date Extraction Date Species
Stacy L Sell, Rebecca M Craft, Patricia K Seitz, Sonja J Stutz, Kathryn A Cunningham, Mary L Thoma. Estradiol-sertraline synergy in ovariectomized rats. Psychoneuroendocrinology. vol 33. issue 8. 2008-12-09. PMID:18650020. immediately after the fst, midbrain, hippocampus and prefrontal cortex tissue was removed and frozen for analysis of gene expression via quantitative real-time pcr (midbrain tissue) and protein expression via western blot (prefrontal cortex and hippocampal tissue). 2008-12-09 2023-08-12 rat
Raffaella Molteni, Matteo Pasini, Stefania Moraschi, Massimo Gennarelli, Filippo Drago, Giorgio Racagni, Marco A Riv. Reduced activation of intracellular signaling pathways in rat prefrontal cortex after chronic phencyclidine administration. Pharmacological research. vol 57. issue 4. 2008-07-29. PMID:18406625. western blot analysis has been performed to determine total and phospho-protein levels of extracellular signal-regulated kinases 1/2 (erk1/2), type ii calcium/calmodulin-dependent protein kinase (alphacamkii) and camp-response element binding protein (creb) in prefrontal cortex (pfc) and hippocampus (hip) of rat chronically treated with pcp, whereas their mrna levels were determined by real time rt-pcr. 2008-07-29 2023-08-12 rat
P Sánchez, J M Torres, P Gavete, E Orteg. Effects of swim stress on mRNA and protein levels of steroid 5alpha-reductase isozymes in prefrontal cortex of adult male rats. Neurochemistry international. vol 52. issue 3. 2008-06-20. PMID:17826869. in this paper, a quantitative rt-pcr method coupled to laser-induced fluorescence capillary electrophoresis (lif-ce) and western blot were used to measure both mrna and protein levels of 5alpha-r type 1 (5alpha-r1) and 5alpha-r type 2 (5alpha-r2) isozymes in prefrontal cortex of male rats after acute swim stress situations. 2008-06-20 2023-08-12 rat
Fabio Fumagalli, Angelisa Frasca, Giorgio Racagni, Marco Andrea Riv. Dynamic regulation of glutamatergic postsynaptic activity in rat prefrontal cortex by repeated administration of antipsychotic drugs. Molecular pharmacology. vol 73. issue 5. 2008-05-06. PMID:18250147. by using western blot and immunoprecipitation techniques, we investigated the expression, trafficking, and interaction of essential components of glutamatergic synapse in rat prefrontal cortex. 2008-05-06 2023-08-12 rat
Ghanshyam N Pandey, Yogesh Dwivedi, Xinguo Ren, Hooriyah S Rizavi, Rosalinda C Roberts, Robert R Conle. Cyclic AMP response element-binding protein in post-mortem brain of teenage suicide victims: specific decrease in the prefrontal cortex but not the hippocampus. The international journal of neuropsychopharmacology. vol 10. issue 5. 2008-01-17. PMID:16978443. we determined cre-dna binding using the gel shift assay, as well as protein expression of creb using the western blot technique, and the mrna expression of creb using a quantitative reverse transcriptase-polymerase chain reaction (rt-pcr) technique in the prefrontal cortex (pfc), and hippocampus obtained from 17 teenage suicide victims and 17 matched normal control subjects. 2008-01-17 2023-08-12 human
Xiaoli Qi, Wenjuan Lin, Junfa Li, Yuqin Pan, Weiwen Wan. The depressive-like behaviors are correlated with decreased phosphorylation of mitogen-activated protein kinases in rat brain following chronic forced swim stress. Behavioural brain research. vol 175. issue 2. 2007-01-11. PMID:17050000. the extracellular signal-regulated kinase (erk) and phospho-erk (p-erk) in the hippocampus and prefrontal cortex were determined using western blot. 2007-01-11 2023-08-12 rat
Grzegorz Kreiner, Adam Bielawski, Agnieszka Zelek-Molik, Marta Kowalska, Irena Nalep. Chronic treatment with citalopram does not affect the expression of alpha1-adrenergic receptor (alpha1-AR) subtypes. Polish journal of pharmacology. vol 56. issue 6. 2006-12-21. PMID:15662097. the receptors density was assessed in the rat cerebral cortex by [3h]prazosin binding while the expression of alpha1a and alpha1b receptors' mrna was measured in the rat prefrontal cortex by northern blot analysis or competitive reverse transcription and polymerase chain reaction (rt-pcr), respectively. 2006-12-21 2023-08-12 rat
M López De Jesús, J Sallés, J J Meana, L F Callad. Characterization of CB1 cannabinoid receptor immunoreactivity in postmortem human brain homogenates. Neuroscience. vol 140. issue 2. 2006-09-25. PMID:16563642. western blot analysis of p1 and p2 fractions, and crude plasma membrane preparations from the prefrontal cortex showed that cb1 migrated as a 60 kda monomer under reducing conditions. 2006-09-25 2023-08-12 human
Daya S Gupta, Robert E McCullumsmith, Monica Beneyto, Vahram Haroutunian, Kenneth L Davis, James H Meador-Woodruf. Metabotropic glutamate receptor protein expression in the prefrontal cortex and striatum in schizophrenia. Synapse (New York, N.Y.). vol 57. issue 3. 2005-10-27. PMID:15945063. accordingly, we measured the expression of metabotropic glutamate receptors (mglurs) in brodmann areas 9, 11, 32, and 46 in the prefrontal cortex (pfc) and the caudate, putamen, and nucleus accumbens in schizophrenia (16 cases, 9 controls) by western blot analysis. 2005-10-27 2023-08-12 Not clear
Jingshan Chen, Barbara K Lipska, Nader Halim, Quang D Ma, Mitsuyuki Matsumoto, Samer Melhem, Bhaskar S Kolachana, Thomas M Hyde, Mary M Herman, Jose Apud, Michael F Egan, Joel E Kleinman, Daniel R Weinberge. Functional analysis of genetic variation in catechol-O-methyltransferase (COMT): effects on mRNA, protein, and enzyme activity in postmortem human brain. American journal of human genetics. vol 75. issue 5. 2005-01-03. PMID:15457404. we analyzed the effects of several single-nucleotide polymorphisms (snps) within comt on mrna expression levels (using reverse-transcriptase polymerase chain reaction analysis), protein levels (using western blot analysis), and enzyme activity (using catechol methylation) in a large sample (n = 108) of postmortem human prefrontal cortex tissue, which predominantly expresses the -membrane-bound isoform. 2005-01-03 2023-08-12 mouse
Brian P Ramos, Shari G Birnbaum, Isabelle Lindenmayer, Samuel S Newton, Ronald S Duman, Amy F T Arnste. Dysregulation of protein kinase a signaling in the aged prefrontal cortex: new strategy for treating age-related cognitive decline. Neuron. vol 40. issue 4. 2004-01-13. PMID:14622586. western blot and immunohistochemical analyses of rat brain further indicate that the camp/pka pathway becomes disinhibited in the prefrontal cortex with advancing age. 2004-01-13 2023-08-12 rat
Grzegorz Kreiner, Marek Sanak, Agnieszka Zelek-Molik, Irena Nalep. Using reverse transcription and a competitive polymerase chain reaction for quantification of alpha1B-adrenoceptor mRNA. Polish journal of pharmacology. vol 54. issue 4. 2003-06-03. PMID:12523494. the goal of the present study was to establish the method of reverse transcription and competitive polymerase chain reaction (rt-cpcr) to quantify a steady state level of alpha1b-ar mrna in the hippocampus, prefrontal cortex and thalamus, and to compare the alpha1b-ar' pattern of expression with that revealed by northern blot analysis. 2003-06-03 2023-08-12 rat
M J Decker, G E Hue, W M Caudle, G W Miller, G L Keating, D B Ry. Episodic neonatal hypoxia evokes executive dysfunction and regionally specific alterations in markers of dopamine signaling. Neuroscience. vol 117. issue 2. 2003-05-21. PMID:12614682. rats were killed on postnatal day 80 and tyrosine hydroxylase, vesicular monoamine transporter, dopamine transporter, and dopamine d1 receptors were quantified in the prefrontal cortex, primary sensorimotor cortex, and precommissural striatum by western blot analyses. 2003-05-21 2023-08-12 rat
Irena Nalepa, Grzegorz Kreiner, Marta Kowalska, Marek Sanak, Agnieszka Zelek-Molik, Jerzy Vetulan. Repeated imipramine and electroconvulsive shock increase alpha 1A-adrenoceptor mRNA level in rat prefrontal cortex. European journal of pharmacology. vol 444. issue 3. 2003-02-11. PMID:12063075. the mrna expression was assessed by northern blot in the prefrontal cortex and the hippocampus, the receptor density was measured by [3h]prazosin binding in the total cerebral cortex and hippocampus. 2003-02-11 2023-08-12 rat
A Czyrak, A Chocyk, M Maćkowiak, K Fijał, K Wedzon. Distribution of dopamine D1 receptors in the nucleus paraventricularis of the hypothalamus in rats: an immunohistochemical study. Brain research. Molecular brain research. vol 85. issue 1-2. 2001-03-08. PMID:11146123. when measured by the western blot technique, the quantity of d1 receptor protein found in the paraventricular nucleus of the hypothalamus was at the level found in the prefrontal cortex. 2001-03-08 2023-08-12 rat
D Zamanillo, F Andreu, S Ovalle, M P Pérez, G Romero, A J Farré, X Guitar. Up-regulation of sigma(1) receptor mRNA in rat brain by a putative atypical antipsychotic and sigma receptor ligand. Neuroscience letters. vol 282. issue 3. 2000-04-19. PMID:10717418. sigma(1) (sigma(1)) receptor mrna expression was studied in the prefrontal cortex, striatum, hippocampus and cerebellum of rat brain by northern blot and in situ hybridization. 2000-04-19 2023-08-12 rat
A M Persico, C W Schindler, R Zaczek, M T Brannock, G R Uh. Brain transcription factor gene expression, neurotransmitter levels, and novelty response behaviors: alterations during rat amphetamine withdrawal and following chronic injection stress. Synapse (New York, N.Y.). vol 19. issue 3. 1995-07-18. PMID:7784961. amphetamine withdrawal-induced decreases in transcription factor mrna levels, assessed using northern blot analysis, appear most prominent in prefrontal cortex, begin approximately 12 h after the last injection, and largely recover to control levels by 54 h. prefrontal cortical and striatal dopamine content, assessed using hplc, decrease and recover over a similar time course. 1995-07-18 2023-08-12 rat
P N Nelson, M Goodall, R Jefferi. Characterisation of putative monoclonal anti-G3m(u) and anti-G3m(g) reagents and their antigenic determinants. Immunological investigations. vol 23. issue 1. 1994-05-05. PMID:7511566. surprisingly anti-g3m(u) mab pnf69c bound to the pfc' of igg3-related protein, hw, and to the pfc' of igg1-related protein, pr, in western blot. 1994-05-05 2023-08-12 Not clear
T Anezaki, K Yanagisawa, H Takahashi, T Nakajima, K Miyashita, A Ishikawa, F Ikuta, T Miyatak. Remote astrocytic response of prefrontal cortex is caused by the lesions in the nucleus basalis of Meynert, but not in the ventral tegmental area. Brain research. vol 574. issue 1-2. 1992-08-28. PMID:1638410. quantitative western blot analysis revealed significantly greater immunoreactivity for gfap in the prefrontal cortex of nbm-lesioned rats. 1992-08-28 2023-08-11 rat