| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Zhenhuan Jiang, Jiannong Jiang, Huilin Yang, Zhijun Ge, Qiang Wang, Leiyan Zhang, Chenguang Wu, Jinzhi Wan. Silencing of Aurora kinase A by RNA interference inhibits tumor growth in human osteosarcoma cells by inducing apoptosis and G2/M cell cycle arrest. Oncology reports. vol 31. issue 3. 2014-09-26. PMID:24452445. |
we further evaluated the effect of aurka silence on cell viability by mtt assay, cell apoptosis and cell cycle by flow cytometer detection. |
2014-09-26 |
2023-08-12 |
human |
| J Bai, L Dong, Z Song, H Ge, X Cai, G Wang, P Li. The role of melatonin as an antioxidant in human lens epithelial cells. Free radical research. vol 47. issue 8. 2014-09-23. PMID:23710615. |
cell viability was monitored by a 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyl-tetrazoliumbromide (mtt) assay, and the apoptosis rate and intracellular reactive oxygen species (ros) levels were measured by flow cytometry using annexin v-fitc and propidium iodide (pi) staining. |
2014-09-23 |
2023-08-12 |
human |
| Monica Catarina Botelho, Carla Costa, Susana Silva, Solange Costa, Alok Dhawan, Paula A Oliveira, João P Teixeir. Effects of titanium dioxide nanoparticles in human gastric epithelial cells in vitro. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 68. issue 1. 2014-09-22. PMID:24051123. |
we analyzed proliferation by mtt assay, apoptosis by tunel, migration by injury assay, oxidative stress by determining gsh/gssg ratio and dna damage by comet assay on nanoparticle-treated ags human gastric epithelial cell line in comparison to controls. |
2014-09-22 |
2023-08-12 |
human |
| Yanbo Li, Caixia Guo, Zhicheng Wang, Pingsheng Gong, Zhiwei Sun, Shouliang Gon. Enhanced effects of TRAIL-endostatin-based double-gene-radiotherapy on suppressing growth, promoting apoptosis and inducing cell cycle arrest in vascular endothelial cells. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 32. issue 2. 2014-09-11. PMID:22528215. |
then mtt assay was used for determining the cellular proliferation, and flow cytometry (fcm) plus annexin v and propidium iodide (pi) double-staining or pi single-staining were employed for the detection of apoptosis and cell cycle progression. |
2014-09-11 |
2023-08-12 |
human |
| Zhengwei Li, Jincao Chen, Ting Lei, Huaqiu Zhan. Tamoxifen induces apoptosis of mouse microglia cell line BV-2 cells via both mitochondrial and death receptor pathways. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 32. issue 2. 2014-09-11. PMID:22528224. |
cell viability was assessed by the mtt assay, and flow cytometric analysis was performed to detect the cell apoptosis rate. |
2014-09-11 |
2023-08-12 |
mouse |
| Miao Wang, Tao Huang, Fuqing Zeng, Guosong Jiang, Liang Wang, Liduan Zheng, Qiangsong Ton. Effect of Smac on TRAIL-induced apoptosis of prostate cancer cell line PC-3 and the molecular mechanism. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 32. issue 2. 2014-09-11. PMID:22528226. |
after treatment with trail as an apoptosis inducer, in vitro cell growth activity was assayed by mtt colorimetry. |
2014-09-11 |
2023-08-12 |
Not clear |
| Yin Xiao, Ping Zou, Jie Wang, Hui Song, Jing Zou, Lingbo Li. Lower phosphorylation of p38 MAPK blocks the oxidative stress-induced senescence in myeloid leukemic CD34(+)CD38 (-) cells. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 32. issue 3. 2014-09-11. PMID:22684553. |
the proliferation and apoptosis of cd34(+)cd38(-) cells were detected by mtt assay and flow cytometry. |
2014-09-11 |
2023-08-12 |
human |
| Lijian Xu, Qiang Li, Dong Xu, Qingguang Wang, Yong An, Qing Du, Jingjing Zhang, Yi Zhu, Yi Mia. hsa-miR-141 downregulates TM4SF1 to inhibit pancreatic cancer cell invasion and migration. International journal of oncology. vol 44. issue 2. 2014-09-11. PMID:24285464. |
sw1990 and bxpc-3 cells were transfected with the inhibitor mir-141, the inhibitor negative control, the mir-141 mimic and the mimic negative control; and cell invasion, migration, proliferation, cell cycle progression and apoptosis were detected by transwell, mtt and flow cytometry assays, respectively. |
2014-09-11 |
2023-08-12 |
human |
| Lijuan Xu, Shuling Zhang, Hongling Fan, Zhichao Zhong, Xi Li, Xiaoxiao Jin, Quanzhong Chan. ClC-3 chloride channel in hippocampal neuronal apoptosis. Neural regeneration research. vol 8. issue 32. 2014-09-10. PMID:25206625. |
neuronal survival was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, and apoptosis was assayed by hoechst 33342-labeled neuronal dna fluorescence staining. |
2014-09-10 |
2023-08-13 |
rat |
| Ting Zou, Xiangqi Tang, Zhiling Huang, Niangui Xu, Zhiping H. The Pael-R gene does not mediate the changes in rotenone-induced Parkinson's disease model cells. Neural regeneration research. vol 9. issue 4. 2014-09-10. PMID:25206827. |
the results of reverse-transcription polymerase chain reaction and western blot analysis showed that pael-r expression was decreased after rna interference compared with the control group (no treatment) and the model group (rotenone treatment), while the rate of apoptosis and survival of dopaminergic cells did not differ significantly between groups, as detected by flow cytometry and an mtt assay. |
2014-09-10 |
2023-08-13 |
Not clear |
| Xiaohui Liu, Xiaodong Peng, Zhenzhen Hu, Qingmei Zhao, Jian He, Junhe Li, Xiaojun Zhon. Effects of over-expression of ANXA10 gene on proliferation and apoptosis of hepatocellular carcinoma cell line HepG2. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 32. issue 5. 2014-09-09. PMID:23073794. |
flow cytometry and mtt assay were performed to examine the changes in cell apoptosis and proliferation respectively. |
2014-09-09 |
2023-08-12 |
human |
| Ruolian Dai, Yuanpeng Xia, Ling Mao, Yuanwu Mei, Yumei Xue, Bo H. Involvement of PI3K/Akt pathway in the neuroprotective effect of Sonic hedgehog on cortical neurons under oxidative stress. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 32. issue 6. 2014-09-09. PMID:23271286. |
the cell viability was measured by mtt and apoptosis rate by flow cytometry analysis. |
2014-09-09 |
2023-08-12 |
rat |
| Jifeng Xiang, Xiang Fu, Wenhua Ran, Xuan Chen, Zhen Hang, Hongchao Mao, Ziwei Wan. Expression and role of grainyhead-like 2 in gastric cancer. Medical oncology (Northwood, London, England). vol 30. issue 4. 2014-09-05. PMID:24068586. |
to further investigate the role of grhl2 in gastric cancer as well as the potential mechanisms, sgc7901 cells were transfected with lentiviral constructs expressing grhl2 or empty vector, and then proliferation and apoptosis of sgc7901 cells were evaluated by mtt assay and flow cytometry, respectively. |
2014-09-05 |
2023-08-12 |
human |
| Eduardo Parra, Jorge Ferreira, Luis Gutierre. Decreased c-Abl activity in PC-3 and LNCaP prostate cancer cells overexpressing the early growth response-1 protein. Oncology reports. vol 31. issue 1. 2014-09-05. PMID:24190424. |
cells overexpressing egr-1 were studied with respect to apoptosis (annexin v)/devdase activity, egr-1/c-abl activation (western blotting) and cell proliferation (mtt assay). |
2014-09-05 |
2023-08-12 |
human |
| Fangfang Zheng, Kaiwen He, Xin Li, Dan Zhao, Fei Sun, Yu Zhang, Dan Nie, Xingda Li, Wenfeng Chu, Yan Sun, Yanjie L. Transient overexpression of TGFBR3 induces apoptosis in human nasopharyngeal carcinoma CNE-2Z cells. Bioscience reports. vol 33. issue 2. 2014-09-04. PMID:23387308. |
effects of tgfbr3 overexpression on cell viability and apoptosis were measured by mtt [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2h-tetrazolium bromide], ao/eb (acridine orange/ethidium bromide) staining and electron microscopy in human npc cne-2z cells. |
2014-09-04 |
2023-08-12 |
human |
| W Sun, R Depping, W Jelkman. Interleukin-1β promotes hypoxia-induced apoptosis of glioblastoma cells by inhibiting hypoxia-inducible factor-1 mediated adrenomedullin production. Cell death & disease. vol 5. 2014-09-02. PMID:24457964. |
apoptosis and mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays showed that am protects glioblastoma cells against hypoxia-induced apoptosis in a dose-dependent manner. |
2014-09-02 |
2023-08-12 |
human |
| Jianhong Hou, Jiantai He, Xin Jin, Tian Hu, Yangde Zhan. Study on optimisation of extraction process of tanshinone IIA and its mechanism of induction of gastric cancer SGC7901 cell apoptosis. African journal of traditional, complementary, and alternative medicines : AJTCAM. vol 10. issue 6. 2014-08-31. PMID:24311868. |
extraction process of tanshinone iia was optimised by orthogonal experimental method, and its effect on gastric cancer sgc7901 cell apoptosis was observed using mtt assay and electron microscopy. |
2014-08-31 |
2023-08-12 |
Not clear |
| Qing-Hua Yao, Xiao-Chen Zhang, Ting Fu, Jian-Zhong Gu, Lu Wang, Yun Wang, Yue-Biao Lai, Yu-Qi Wang, Yong Gu. ω-3 polyunsaturated fatty acids inhibit the proliferation of the lung adenocarcinoma cell line A549 in vitro. Molecular medicine reports. vol 9. issue 2. 2014-08-26. PMID:24276408. |
a methyl thiazolyl tetrazolium (mtt) assay was used to detect cell proliferation, apoptosis was detected by flow cytometry and morphological analysis was determined by fluorescence microscopy and transmission electron microscopy. |
2014-08-26 |
2023-08-12 |
human |
| Seon-Young Park, Ji-Young Kim, Su-Mi Lee, Chung-Hwan Jun, Sung-Bum Cho, Chang-Hwan Park, Young-Eun Joo, Hyun-Soo Kim, Sung-Kyu Choi, Jong-Sun Re. Capsaicin induces apoptosis and modulates MAPK signaling in human gastric cancer cells. Molecular medicine reports. vol 9. issue 2. 2014-08-26. PMID:24337453. |
results of the mtt assay and flow cytometry revealed that capsaicin potentially inhibited the proliferation of ags cells and induced apoptosis in vitro in a dose-dependent manner. |
2014-08-26 |
2023-08-12 |
human |
| Ravikanth Danda, Gopinath Krishnan, Kalaivani Ganapathy, Uma Maheswari Krishnan, Khetan Vikas, Sailaja Elchuri, Nivedita Chatterjee, Subramanian Krishnakuma. Targeted expression of suicide gene by tissue-specific promoter and microRNA regulation for cancer gene therapy. PloS one. vol 8. issue 12. 2014-08-25. PMID:24391761. |
the cell proliferation was measured by mtt assay, apoptosis was measured by probing cleaved caspase3, epcam and tk expression were quantified by western blot. |
2014-08-25 |
2023-08-12 |
Not clear |