| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Karla Melo, Carolina Ramos Dos Santos, Edna Cristina Santos Franco, Arnaldo Jorge Martins Filho, Samir Mansour Moraes Casseb, Pedro Fernando da Costa Vasconcelo. Exploring the interplay between miRNAs, apoptosis and viral load, in Dengue virus infection. Virology. vol 596. 2024-05-18. PMID:38761641. |
methodologically, the study employs quantitative pcr for viral load and mirna expression analysis, and western blot for apoptosis and bcl2 detection, with a statistical framework that includes anova and correlation analysis to discern significant differences and relationships. |
2024-05-18 |
2024-05-27 |
Not clear |
| Shuang Gao, Na Li, Zhongjing Lin, Yisheng Zhong, Yanuo Wang, Xi She. Inhibition of NLRP3 inflammasome by MCC950 under hypoxia alleviates photoreceptor apoptosis via inducing autophagy in Müller glia. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. vol 38. issue 10. 2024-05-16. PMID:38752538. |
retinal autophagy, apoptosis, and related pathways were evaluated by western blot, immunofluorescent labeling, transmission electron microscopy, and tunel assay. |
2024-05-16 |
2024-05-27 |
mouse |
| Shuang Gao, Na Li, Zhongjing Lin, Yisheng Zhong, Yanuo Wang, Xi She. Inhibition of NLRP3 inflammasome by MCC950 under hypoxia alleviates photoreceptor apoptosis via inducing autophagy in Müller glia. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. vol 38. issue 10. 2024-05-16. PMID:38752538. |
autophagic activity in müller glia after nlrp3 inflammasome inhibition, together with its influence on photoreceptor death, was studied using western blot, immunofluorescence staining, mrfp-gfp-lc3 adenovirus transfection, cell viability, proliferation, and apoptosis assays. |
2024-05-16 |
2024-05-27 |
mouse |
| Mitesh Joshi, Purvi Bhat. Ameliorated in vitro anti-cancer efficacy of methotrexate loaded zinc oxide nanoparticles in breast cancer cell lines MCF-7 & MDA-MB-231 and its acute toxicity study. Nanotechnology. 2024-05-15. PMID:38746972. |
in vitro assays, including cell cycle analysis, apoptosis assay, and western blot analysis to study the protein expression were performed. |
2024-05-15 |
2024-05-27 |
Not clear |
| Xiaofan Yang, Yuling Ni. CTRP 9 mitigates the apoptosis and unfolded protein response of OGD/R-induced retinal ganglion cells by regulating the AMPK pathway. Polish journal of pathology : official journal of the Polish Society of Pathologists. vol 75. issue 1. 2024-05-14. PMID:38741428. |
flow cytometry, enzyme-linked immunosorbent assay and western blot assay were performed to assess cell apoptosis. |
2024-05-14 |
2024-05-27 |
Not clear |
| Nathalie Wilke, Corazon Frias, Albrecht Berkessel, Aram Proko. (2,6-Dimethylphenyl)arsonic Acid Induces Apoptosis through the Mitochondrial Pathway, Downregulates XIAP, and Overcomes Multidrug Resistance to Cytostatic Drugs in Leukemia and Lymphoma Cells In Vitro. International journal of molecular sciences. vol 25. issue 9. 2024-05-11. PMID:38731935. |
mechanistic investigations of as2-mediated apoptosis involving fadd (fas-associated death domain)-deficient or smac (second mitochondria-derived activator of caspases)/diablo (direct iap binding protein with low pi)-overexpressing cell lines, western blot analysis of caspase-9 cleavage, and measurements of mitochondrial membrane integrity identified the mitochondrial apoptosis pathway as the main mode of action. |
2024-05-11 |
2024-05-27 |
Not clear |
| Ran Lee, Won-Young Lee, Hyun-Jung Par. Anticancer Effects of Mitoquinone via Cell Cycle Arrest and Apoptosis in Canine Mammary Gland Tumor Cells. International journal of molecular sciences. vol 25. issue 9. 2024-05-11. PMID:38732133. |
these findings were supported using western blot analysis, demonstrating elevated levels of cleaved caspase-3, a hallmark of apoptosis, and decreased expression of cyclin-dependent kinase (cdk) 2 and cyclin d4, pivotal regulators of the cell cycle. |
2024-05-11 |
2024-05-27 |
human |
| Shujiang Zhuo, Sufei Song, Chaoyi Wang, Zhe Wang, Ming Zhang, Daobin Lin, Kaili Che. Inflammatory corpuscle AIM2 facilitates macrophage foam cell formation by inhibiting cholesterol efflux protein ABCA1. Scientific reports. vol 14. issue 1. 2024-05-11. PMID:38734775. |
the intracellular cholesterol efflux rate was detected by scintillation counting; high-performance liquid chromatography (hplc) was used to detect intracellular cholesterol levels; apoptosis levels were detected by tunel kit; levels of inflammatory markers (il-1β, il-18, ros, and gsh) were detected by elisa kits; and levels of aim2 and abca1 proteins were detected by western blot. |
2024-05-11 |
2024-05-27 |
Not clear |
| Fang Bian, Fan-Hong Niu, Ping-Yuan Qu, Fang Gong, Jian-Zhou Ya. Glycyrrhizic acid inhibits DNA damage repair and enhances cisplatin-induced apoptosis of melanoma cells. Chemical biology & drug design. vol 103. issue 5. 2024-05-10. PMID:38725079. |
after that, cell counting kit-8 (cck-8) was employed for testing the cell viability, flow cytometry for the apoptosis, comet assay for the dna damage of cells, and western blot for the cleaved-caspase3, caspase3, bcl-2, and γh2ax protein expression levels. |
2024-05-10 |
2024-05-27 |
human |
| Jinchuan Zhao, Ping Yang, L I Lu, Tao Yi, Yunfeng Li, Wei Mao, Qin Zhou, K E Li. A study on expression of GRP78 and CHOP in neutrophil endoplasmic reticulum and their relationship with neutrophil apoptosis in the development of sepsis. Journal of biosciences. vol 49. 2024-05-10. PMID:38726820. |
the expressions of endoplasmic reticulum specific glucose regulatory protein 78 (grp78), c/ebp homologous protein (chop), apoptosis signal-regulating kinase 1 (ask1), bcl-2-like 11 (bim), death receptor 5 (dr5), c-jun n-terminal kinases (jnk) and p38 were detected by western blot and pcr. |
2024-05-10 |
2024-05-27 |
human |
| Xian Chang, Ya Cao, Zhi-Lei Hu, Yu Zhai, Yu-Yao Zhang, Yang-Fan Lv, Chang-Qing L. PHLDA2 overexpression facilitates senescence and apoptosis via the mitochondrial route in human nucleus pulposus cells by regulating Wnt/β-catenin signalling pathway. IUBMB life. 2024-05-09. PMID:38721892. |
senescence and apoptosis of npcs were assessed by sa-β-galactosidase, flow cytometry and western blot. |
2024-05-09 |
2024-05-27 |
human |
| Haiyang Guo, Jinghao Shu, Guangbing Hu, Bingyang Liu, Jie Li, Jinhong Sun, Xiaobo Wang, Han Liu, Shiyu Xiong, Yong Tang, Yaolin Yin, Xianfei Wan. Downregulation of RCN1 inhibits esophageal squamous cell carcinoma progression and M2 macrophage polarization. PloS one. vol 19. issue 5. 2024-05-07. PMID:38713738. |
the expression levels of epithelial-mesenchymal transition (emt)-related proteins were evaluated by western blot, while apoptosis was detected by flow cytometry and western blot. |
2024-05-07 |
2024-05-27 |
Not clear |
| Miao Ding, Yu Yang, Ziyang Zhang, Hongxiang Liu, Yingdi Dai, Zixuan Wang, Sijia Ma, Yang Liu, Qi Wan. Structural characterization of the polysaccharide from the black crystal region of Inonotus obliquus and its effect on AsPC-1 and SW1990 pancreatic cancer cell apoptosis. International journal of biological macromolecules. 2024-04-27. PMID:38677687. |
furthermore, western blot analysis confirmed that iop1-1 could induce apoptosis in aspc-1 and sw1990 pancreatic cancer cells through three pathways: the mitochondrial pathway, the death receptor pathway, and endoplasmic reticulum stress. |
2024-04-27 |
2024-04-30 |
Not clear |
| Menghuan Yang, Jun Jiang, Ruimin Ren, Ning Gao, Jun He, Yuebo Zhan. Role of ADAR1 on Proliferation and Differentiation in Porcine Preadipocytes. Animals : an open access journal from MDPI. vol 14. issue 8. 2024-04-27. PMID:38672349. |
in this study, we investigated the role of adar1 in differentiation, proliferation and apoptosis of porcine preadipocytes using cck-8, edu staining, cell cycle detection, rt-qpcr, western blot, a triglyceride assay and oil red o staining. |
2024-04-27 |
2024-04-29 |
mouse |
| Rak Ho Son, Myeong Il Kim, Hye Mi Kim, Shuo Guo, Do Hyun Lee, Gyu Min Lim, Seong-Min Kim, Jae-Yong Kim, Chul Young Ki. Potential of Lycii Radicis Cortex as an Ameliorative Agent for Skeletal Muscle Atrophy. Pharmaceuticals (Basel, Switzerland). vol 17. issue 4. 2024-04-27. PMID:38675422. |
we evaluated the effect of lrce on improving muscle atrophy using a variety of methods, including immunofluorescence staining, quantitative polymerase chain reaction (qpcr), western blot, measurements of oxidative stress, apoptosis, atp levels, and muscle tissue analysis. |
2024-04-27 |
2024-04-29 |
mouse |
| P G Roopashree, Shilpa S Shetty, Vijith Vittal Shetty, P C Suhasini, Kumari N Sucheth. Inhibitory effects of medium-chain fatty acids on the proliferation of human breast cancer cells via suppression of Akt/mTOR pathway and modulating the Bcl-2 family protein. Journal of cellular biochemistry. 2024-04-26. PMID:38666486. |
to investigate the apoptosis-inducing effect of mcfas in breast cancer cells, cell viability assay, colony formation assay, cell migration assay, cell invasion assay, nuclear morphology, cell cycle assay, intracellular reactive oxygen species (ros), matrix metalloproteinase (mmp), apoptosis, rt-qpcr analysis, and western blot analysis were performed. |
2024-04-26 |
2024-04-28 |
human |
| Wei-Zhe Liang, Yuan-Yi Chia, Huai-Jhih Sun, Gwo-Ching Su. Exploration of beauvericin's toxic effects and mechanisms in human astrocytes and N-acetylcysteine's protective role. Toxicon : official journal of the International Society on Toxinology. 2024-04-26. PMID:38670497. |
western blot analysis of cells treated with bea revealed altered protein levels of bax, cleaved caspase-9, and caspase-3, along with an increased bax/bcl-2 ratio, indicating the induction of apoptosis. |
2024-04-26 |
2024-04-29 |
human |
| Jiuming Dai, Jin Liu, Yucheng Shen, Bing Zhang, Chaonian Li, Zhidong Li. Regulation of endoplasmic reticulum stress on autophagy and apoptosis of nucleus pulposus cells in intervertebral disc degeneration and its related mechanisms. PeerJ. vol 12. 2024-04-26. PMID:38666076. |
cell apoptosis was detected by flow cytometry and the expression of lc3ii/i was detected by western blot. |
2024-04-26 |
2024-04-28 |
Not clear |
| Jiuming Dai, Jin Liu, Yucheng Shen, Bing Zhang, Chaonian Li, Zhidong Li. Regulation of endoplasmic reticulum stress on autophagy and apoptosis of nucleus pulposus cells in intervertebral disc degeneration and its related mechanisms. PeerJ. vol 12. 2024-04-26. PMID:38666076. |
then, above functional experiments were conducted again and western blot was employed for the evaluation of autophagy-, apoptosis and ers-related proteins. |
2024-04-26 |
2024-04-28 |
Not clear |
| Sheng-Yun Wu, Ke-Ru Cheng, Yan-Yun Zhou, Yin-Fang Wan. [Microtubule-associated tumor suppressor 1 inhibits hemin-induced apoptosis of vascular endothelial cells via hemeoxygenase 1]. Sheng li xue bao : [Acta physiologica Sinica]. vol 76. issue 2. 2024-04-25. PMID:38658371. |
the effect of mtus1 or hmox1 knockdown on hemin-induced apoptosis in huvec, and the overexpression of nrf2 on hemin-induced apoptosis in mtus1 knockdown 293t cells were assayed with cck8 and western blot. |
2024-04-25 |
2024-04-28 |
human |