| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Han Wang, Yonghao Xiao, Yuanguo Zhang, Zhu Meng, Chenyu Zhao, Fanshan Qiu, Chongchong Li, Zengguo Fen. Study on the effect of type III recombinant humanized collagen on human vascular endothelial cells. Tissue engineering. Part C, Methods. 2023-11-29. PMID:38019085. |
the effect of hcolii on proliferation of ea.hy926 cells was detected by mtt assay, the effect of hcolii on cell migration was investigated by scratch assay, the effect of hcoliii on cell cycle and apoptosis was detected by flow cytometry, the ability of hcolii to induce angiogenesis of ea.hy926 cells was evaluated by angiogenesis assay, and the effect of hcolii on vegf expression was detected by rt-pcr analysis. |
2023-11-29 |
2023-12-07 |
human |
| Jie Wang, Hao Yin, Wei Zhu, Qingyi He, Haitang Zhang, Lu Sun, Yunxiao Qiao, Yanwei Xian. Research on the resistance of isoviolanthin to hydrogen peroxide-triggered injury of skin keratinocytes based on Transcriptome sequencing and molecular docking. Medicine. vol 102. issue 47. 2023-11-28. PMID:38013320. |
in this study, methyl thiazolyl tetrazolium (mtt) assay and western blots were used to investigate the skin-protective effect of isoviolanthin, a di-c-glycoside derived from dendrobium officinale, on hydrogen peroxide (h2o2)-triggered apoptosis of skin keratinocytes. |
2023-11-28 |
2023-11-29 |
human |
| Muttiah Barathan, Naveen Kumar Hawala Shivashekaregowda, See Mee Hoong, Kumutha Malar Vellasamy, Jamuna Vadivel. Anticancer effect of aromatic isoniazid derivatives in human gastric adenocarcinoma cells. Toxicology and applied pharmacology. 2023-11-25. PMID:38007073. |
the cytotoxic effect of ithb4 has been determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) assay and further confirmed for anticancer properties by means of apoptosis, reactive oxygen species (ros), nuclear fragmentation, lactate dehydrogenase (ldh), caspases, cytokines and morphological including phenotypic changes of cells assay. |
2023-11-25 |
2023-11-28 |
human |
| Jian Hao, Zhenhan Li, Li Xie, Bingbing Yu, Boyuan Ma, Yubiao Yang, Xuchen Ma, Bitao Wang, Xianhu Zho. Syringaresinol promotes the recovery of spinal cord injury by inhibiting neuron apoptosis via activating the ubiquitination factor E4B/AKT Serine/Threonine kinase signal pathway. Brain research. 2023-11-22. PMID:37992795. |
second, mtt was employed to evaluate cell proliferation rate, western blot was performed to detect protein expression, rt-qpcr was conducted to detect mrna expression levels, flow cytometry and 5-ethynyl-2'-deoxyuridine (edu) staining were used to determine cell apoptosis, and immunofluorescence and immunohistochemistry were used to estimate the expression of rna binding fox-1 homolog 3 and clipped caspase 3. |
2023-11-22 |
2023-11-29 |
rat |
| Mussa Makran, Guadalupe Garcia-Llatas, Amparo Alegría, Antonio Cill. Ethylcoprostanol modulates colorectal cancer cell proliferation and mitigates cytotoxicity of cholesterol metabolites in non-tumor colon cells. Food & function. 2023-11-20. PMID:37982821. |
ethylcoprostanol reduced the tumor cell proliferation (mtt), showing in flow cytometry assays induction of apoptosis |
2023-11-20 |
2023-11-29 |
human |
| Long Zhao, Jianheng Zheng, Jin Qin, Xin Xu, Xinyuan Liu, Shuyu Yang, Shuguang Li, Bo Chen, Jun Du, Ruihua Don. Combined Astragalus, vitamin C, and vitamin E alleviate DEHP-induced oxidative stress and the decreased of insulin synthesis and secretion in INS-1 cells. Ecotoxicology and environmental safety. vol 268. 2023-11-20. PMID:37984288. |
the cell proliferation inhibition rate was determined using mtt assay for each group, and the cell apoptosis rate and intracellular ros level were measured using flow cytometer. |
2023-11-20 |
2023-11-29 |
rat |
| Huifang Zhang, Yudan Zhang, Kaixuan Xu, Lawen Wang, Xin Zhou, Mingge Yang, Jiangli Xie, Hui L. Inhibition of FLT1 Attenuates Neurodevelopmental Abnormalities and Cognitive Impairment in Offspring Caused by Maternal Prenatal Stress. Applied biochemistry and biotechnology. 2023-11-18. PMID:37979086. |
the apoptosis level of hippocampal neurons and the expression of nmdars were detected by mtt assay, tunel assay, and western blot. |
2023-11-18 |
2023-11-20 |
rat |
| Ji He, Piyush Bugde, Jiawei Li, Riya Biswas, Siting Li, Xuewei Yang, Fang Tian, Zimei Wu, Yan L. Multidrug resistance protein 5 affects cell proliferation, migration and gemcitabine sensitivity in pancreatic cancer MIA Paca‑2 and PANC‑1 cells. Oncology reports. vol 51. issue 1. 2023-11-17. PMID:37975256. |
mrp5‑regulated gemcitabine sensitivity was assessed by mtt, prestoblue and apoptosis assays. |
2023-11-17 |
2023-11-20 |
human |
| Ying-Jie Zhu, Hu-Lin Chen, Xin-Jie Cai, Bang-le Zhan, Xiao-Ming Li. Naringin acts as a TRPV1 antagonist to attenuate UVB-induced senescence and damage in HaCaT cells. Chemical biology & drug design. 2023-11-09. PMID:37945519. |
analysis was performed using the mtt assay to assess cell viability, flow cytometry to measure the apoptosis level, sa-β-gal staining to observe cellular senescence, and western blot to assess protein levels of trpv1, p16, p53, p21, matrix metalloproteinase (mmp)-1, and mmp-9. |
2023-11-09 |
2023-11-20 |
human |
| Yiyang Li, Nyuk Ling Ma, Huiling Chen, Jiateng Zhong, Dangquan Zhang, Wanxi Peng, Su Shiung Lam, Yafeng Yang, Xiaochen Yue, Lijun Yan, Ting Wang, Bjarne Styrishave, Tomasz Maciej Ciesielski, Christian Sonn. High-throughput screening of ancient forest plant extracts shows cytotoxicity towards triple-negative breast cancer. Environment international. vol 181. 2023-11-04. PMID:37924601. |
first, to study the effects on viability of the plant extracts, we used a mtt and cck-8 cytotoxicity assay employing triple-negative breast cancer (tnbc) mda-mb-231 and normal epithelial mcf-10a cell lines and cell cycle arrest to estimate apoptosis using flow cytometry for the most potent three speices. |
2023-11-04 |
2023-11-08 |
Not clear |
| Zhen Liu, Hong Zhang, Xiaoxia H. Analysis of the expression and mechanism of follistatin‑like protein 1 in cervical cancer. Oncology reports. vol 50. issue 6. 2023-10-27. PMID:37888756. |
the effects of abnormal expression of fstl1 on cervical cancer cells were assessed using colony formation, mtt, wound‑healing, transwell, apoptosis, and nude mouse tumorigenicity assays. |
2023-10-27 |
2023-11-08 |
mouse |
| Albert Armoo, Tanner Diemer, Abigail Donkor, Jerrod Fedorchik, Severine Van Slambrouck, Rachel Willand-Charnley, Brian A Logu. Methimazole, an Effective Neutralizing Agent of the Sulfur Mustard Derivative 2-Chloroethyl Ethyl Sulfide. ACS bio & med chem Au. vol 3. issue 5. 2023-10-26. PMID:37876493. |
cell viability, relative loss of extracellular matrix adhesions, and apoptosis caused by cees were measured via mtt, cell-matrix adhesion (cma), and apoptosis protein marker assays, respectively. |
2023-10-26 |
2023-11-08 |
human |
| Chuntang Qin, Shuyan Liu, Weibin Chen, Dan Xue, Tianli Guo, Baojiang W. HuR-induced circ_0082319 contributes to hepatocellular carcinoma by elevating PTK2 through miR-505-3p. Naunyn-Schmiedeberg's archives of pharmacology. 2023-10-25. PMID:37878047. |
cell viability, proliferation, apoptosis, invasion, and angiogenesis were measured using (4-5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) assay, 5-ethynyl-2'-deoxyuridine (edu), flow cytometry, transwell, and tube formation assays. |
2023-10-25 |
2023-11-08 |
human |
| Xinchan Jiang, Xiaobo Che. Endometrial cell‑derived exosomes facilitate the development of adenomyosis via the IL‑6/JAK2/STAT3 pathway. Experimental and therapeutic medicine. vol 26. issue 5. 2023-10-24. PMID:37869633. |
mtt, flow cytometry and wound-healing assays were performed to examine am cell proliferation, apoptosis, cell cycle distribution and migration. |
2023-10-24 |
2023-11-08 |
Not clear |
| Zhigang Yang, Liguo Yang, Jun Zhang, Chenzeyue Qian, Yi Zha. AS602801 treatment suppresses breast cancer metastasis to the brain by interfering with gap-junction communication by regulating Cx43 expression. Drug development research. 2023-10-20. PMID:37859299. |
mtt (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay and flow cytometry were performed to evaluate the proliferation and apoptosis of breast cancer cells co-cultivated with astrocytes. |
2023-10-20 |
2023-11-08 |
mouse |
| Stephanie Verbeke, Aurelien Bourdon, Jean-Philippe Guégan, Laura Leroy, Vanessa Chaire, Elodie Richard, Alban Bessede, Antoine Italian. Antitumor effects of PRMT5 inhibition in sarcomas. Cancer research communications. 2023-10-20. PMID:37861293. |
we then used the potent and selective gsk3326595 (gsk595) compound to investigate the antitumor effect of the pharmacological inhibition of prmt5 in vitro via mtt, apoptosis, cell cycle, clonogenicity and proliferation assays. |
2023-10-20 |
2023-11-08 |
Not clear |
| Shahbaz Ahmed, Masood Nadeem, Irfan Hussain, Sana Fatima, Saleha Anwar, Moshahid Alam Rizvi, Md Imtaiyaz Hassan, Mohammad Tabis. Preparation of nanoformulation of 5-fluorouracil to improve anticancer efficacy: integrated spectroscopic, docking, and MD simulation approaches. Journal of biomolecular structure & dynamics. 2023-10-18. PMID:37850451. |
the cytotoxicity and apoptosis profiles confirmed that n5-fu enhanced the anticancer effect of the pure drug in hct-116 cells, as evident from mtt assay, fluorescence microscopy, and facs analysis. |
2023-10-18 |
2023-11-08 |
Not clear |
| Sara Cheleschi, Nicola Veronese, Serafino Carta, Giulia Collodel, Maria Bottaro, Elena Moretti, Roberta Corsaro, Marcella Barbarino, Antonella Fioravant. MicroRNA as Possible Mediators of the Synergistic Effect of Celecoxib and Glucosamine Sulfate in Human Osteoarthritic Chondrocyte Exposed to IL-1β. International journal of molecular sciences. vol 24. issue 19. 2023-10-14. PMID:37834442. |
cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, apoptosis and reactive oxygen species (ros) by cytometry, nitric oxide (no) by griess method. |
2023-10-14 |
2023-10-15 |
human |
| Su-Ji Jeon, Eun-Young Choi, Eun-Ji Han, Sang-Woo Lee, Jun-Mo Moon, Soo-Hyun Jung, Ji-Youn Jun. Piperlongumine induces apoptosis via the MAPK pathway and ERK‑mediated autophagy in human melanoma cells. International journal of molecular medicine. vol 52. issue 6. 2023-10-13. PMID:37830157. |
when pl was applied following treatment with autophagy inhibitors 3‑methyladenine and hydroxychloroquine, autophagy exhibited a cytoprotective effect against apoptosis in mtt assay. |
2023-10-13 |
2023-10-15 |
human |
| Zheng Li, Weiwei Zhang, Ji Xu, Xiaoye M. Cdk1 protects against oxygen-glucose deprivation and reperfusion-induced Golgi fragmentation and apoptosis through mediating GM130 phosphorylation. Journal of molecular histology. 2023-10-13. PMID:37831422. |
cell proliferation and apoptosis were determined using mtt assay, tunel staining, and flow cytometry. |
2023-10-13 |
2023-10-15 |
mouse |