| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Monosha Priyadarshini, N Arunai Nambi Ra. Green Synthesis and In Vitro Photodynamic Efficacy of Hypericin: Cytotoxicity Assessment on MCF-7 Breast Cancer Cells. Photodiagnosis and photodynamic therapy. 2024-11-23. PMID:39579841. |
mtt assay and ao-eb staining assay established that hypericin exhibited the highest cell death in mcf7 cancer cells via apoptosis. |
2024-11-23 |
2024-11-26 |
human |
| Sonia Scarfì, Vanessa Almonti, Serena Mirata, Mario Passalacqua, Stefania Vernazza, Janki Prakash Patel, Martin Brook, Ayrton Hamilton, Melanie Kah, Alessandro F Gualtier. In vitro cyto- and geno-toxicity of asbestiform erionite from New Zealand. Environmental research. 2024-11-23. PMID:39579848. |
the cytotoxicity/genotoxicity/inflammatory potential was determined by: (i) analysis of the cytotoxic potential by mtt tests on human cell lines mimicking primary cells making direct contact with fibres in the lungs, combined with apoptosis tests and cell membrane damage by fluorescence microscopy analyses; (ii) analysis of the genotoxic potential by quantification of dna damage measuring double strand break foci by γ-h2ax nuclear staining in confocal microscopy analyses; (iii) analyses of the acute (24-72h) and early-chronic (7d) inflammatory effect by gene expression analyses of several cytokines, as well as of fibrotic and epithelial to mesenchymal transition (emt) markers. |
2024-11-23 |
2024-11-26 |
human |
| Yaser Ebrahimi, Jafar Rezaie, Ali Akbari, Yousef Rasm. Green hydrothermal synthesis of gallic acid carbon dots: characterization and cytotoxic effects on colorectal cancer cell line. Biomedical physics & engineering express. vol 11. issue 1. 2024-11-21. PMID:39530648. |
the cytotoxicity of ga and ga-cds on hct-116 cells was evaluated using the mtt assay at various concentrations over 24 and 48 h. cellular uptake was assessed via fluorescence microscopy, and apoptosis was analyzed using acridine orange/propidium iodide (ao/pi) staining. |
2024-11-21 |
2024-11-23 |
Not clear |
| Zeping Luo, Liwei Pa. Synthesis of Formononetin Derivatives and Cardioprotective Effects. Chemical & pharmaceutical bulletin. vol 72. issue 11. 2024-11-17. PMID:39551522. |
the protective effects of these novel compounds on h9c2 cells were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) method, while the apoptosis rate, myocardial enzyme activity, and autophagy reaction post-compound treatment were assessed using kit-based methods. |
2024-11-17 |
2024-11-22 |
Not clear |
| Hui Wang, Chao Li, Song Meng, Yu-Ting Kuan. The LINC01094/miR-545-3p/SLC7A11 Signaling Axis Promotes the Development of Gastric Cancer by Regulating Cell Growth and Ferroptosis. Biochemical genetics. 2024-11-14. PMID:39540959. |
functional experiments (mtt assay, colony formation assay, and flow cytometry) were conducted to assess the effect of linc01094 and mir-545-3p on cell proliferation, viability, apoptosis, cell cycle, and reactive oxygen species. |
2024-11-14 |
2024-11-17 |
Not clear |
| Adrian Zając, Joanna Sumorek-Wiadro, Aleksandra Maciejczyk, Michał Chojnacki, Iwona Wertel, Wojciech Rzeski, Joanna Jakubowicz-Gi. The engagement of Ras/Raf/MEK/ERK and PLCγ1/PKC pathways regulated by TrkB receptor in resistance of glioma cells to elimination upon apoptosis induction. Neuropharmacology. vol 262. 2024-11-12. PMID:39521041. |
the tested inhibitors single and simultaneous action with temozolomide affection on apoptosis induction was analyzed by mtt, microscopic observations and flow cytometry. |
2024-11-12 |
2024-11-17 |
human |
| Hyun Jung Lee, Ji Wook Moon, Seong-Joon Koh, Jong Pil Im, Byeong Gwan Kim, Joo Sung Ki. Effect of tegoprazan, a novel potassium-competitive acid blocker, on non-steroidal anti-inflammatory drug (NSAID)-induced enteropathy. Scientific reports. vol 14. issue 1. 2024-11-08. PMID:39511288. |
cell viability, expression levels of tight-junction proteins, levels of proinflammatory cytokines, and apoptosis were assessed by conducting mtt assays, rt-pcr, western blotting, and immunofluorescence staining, respectively. |
2024-11-08 |
2024-11-16 |
human |
| Lifan Zhong, Qianru Wang, Zhixiong Kou, Lianfang Gan, Zhaoxin Yang, Junhua Pan, Ling Huang, Yunqiang Che. The combination of FLCWK with 5-FU inhibits colon cancer and multidrug resistance by activating PXR to suppress the IL-6/STAT3 pathway. Journal of cellular and molecular medicine. vol 28. issue 21. 2024-11-04. PMID:39495702. |
by means of mtt assays, flow cytometry apoptosis analysis, western blotting and immunofluorescence, we probed into the prospective impacts of flcwk and 5-fu on cellular viability and resistance. |
2024-11-04 |
2024-11-07 |
mouse |
| Stuti Biswal, Munmun Panda, Bijesh Kumar Biswa. Shikonin Stimulates Mitochondria-Mediated Apoptosis by Enhancing Intracellular Reactive Oxygen Species Production and DNA Damage in Oral Cancer Cells. Journal of cellular biochemistry. 2024-11-01. PMID:39485022. |
we investigated the antiproliferative, antimigratory, cell cycle arresting and apoptosis promoting activity of shk in oral cancer cells by performing mtt and morphological assay, colony, and tumor sphere formation assay, ao/etbr and dapi staining, annexin v-fitc/pi staining, assay for reactive oxygen species (ros) and mitochondrial membrane potential (mmp) measurement, comet assay, qrt-pcr, and western blot analysis. |
2024-11-01 |
2024-11-03 |
Not clear |
| Bader Huwaimel, Kareem M Younes, Amr S Abouzied, Akram M Elkashlan, Fawaz N Alheibshy, Ahmed Alobaida, Abdullah Turki, Saleh A Alquwaiay, Naif Alqahatani, Sulaiman A Alsuwayag. Phytochemical composition, in vitro cytotoxicity, and in silico docking properties of Tamarix tetragyna L. Scientific reports. vol 14. issue 1. 2024-10-27. PMID:39462121. |
in addition, in vitro cytoxicity activity versus cancer cell lines such mcf-7, hepg-2, hct-116, and a-549 was examined by mtt assay method, together with exploring its apoptosis effect by flow cytometry and western blot analysis techniques. |
2024-10-27 |
2024-10-29 |
Not clear |
| Yun Xiang, Xi Cheng, Hong Li, Wenjing Xu, Weiqiang Zhan. Long Non-coding RNA FOXD2-AS1 Silencing Inhibits Malignant Behaviors of Ovarian Cancer Cells Via miR-324-3p/SOX4 Signaling Axis. Reproductive sciences (Thousand Oaks, Calif.). 2024-10-25. PMID:39455487. |
cell viability, apoptosis, migration, along invasion were assessed by mtt, flow cytometry, as well as transwell assays, respectively. |
2024-10-25 |
2024-10-29 |
Not clear |
| Liang Xiao, Haixia Xu, Meixiang Li, Lin Lin, Yan Zh. CXCR4 up-regulation mediated by USP1 deubiquitination promotes the tumorigenesis and immune escape in esophageal squamous-cell carcinoma. Journal of biochemical and molecular toxicology. vol 38. issue 11. 2024-10-23. PMID:39440461. |
mtt assay, 5-ethynyl-2'-deoxyuridine assay, flow cytometry, transwell assay and elisa analysis were used to detect cell oncogenic phenotypes, macrophage phenotypes, inflammatory cytokines production, the cytotoxicity of cytokine-induced killer (cik) cells and cd8 + t cell apoptosis. |
2024-10-23 |
2024-10-25 |
mouse |
| Li Li, Hui Cheng, Yang Peng, Dihong Tan. Targeting Mitochondrial Cholesterol Efflux via TCF21/ABCA10 Pathway to Enhance Cisplatin Efficacy in Ovarian Cancer. Biochemical genetics. 2024-10-22. PMID:39438390. |
cell proliferation was detected by cck-8 and edu staining, flow cytometry for apoptosis, mtt for metabolic activity, calcium-induced cytochrome c release, and mitochondrial matrix swelling for mitochondrial function and oil red o staining for lipid accumulation. |
2024-10-22 |
2024-10-25 |
human |
| Aizhen Li, Mingjin Yang, Wenbiao Duan, Bo W. Dihydrotanshinone I induces necroptosis and cell cycle arrest in gastric cancer through the PTPN11/p38 pathway. Toxicology in vitro : an international journal published in association with BIBRA. 2024-10-20. PMID:39427814. |
in this study, mtt assays, apoptosis detection, immunofluorescence, and functional studies were used to elucidate the mechanisms underlying the effects of dihydrotanshinone i (dht) on gastric cancer cells. |
2024-10-20 |
2024-10-23 |
Not clear |
| Janmejay Pant, Lovedeep Singh, Payal Mittal, Nitish Kuma. Valencene as a novel potential downregulator of THRB in NSCLC: network pharmacology, molecular docking, molecular dynamics simulation, ADMET analysis, and in vitro analysis. Molecular diversity. 2024-10-19. PMID:39425858. |
in vitro assays measured cell viability in a549 and hel 299 cells via mtt assays, assessed apoptosis through hoechst staining, and evaluated mitochondrial potential with jc-1. |
2024-10-19 |
2024-10-22 |
Not clear |
| K Bontor, B Gabrye. Sulodexide protects endothelial cells against 4-hydroxynonenal-induced oxidative stress and glutathione-dependent redox imbalance by modulation of sestrin2/nuclear factor erythroid 2-related factor 2 pathway. Journal of physiology and pharmacology : an official journal of the Polish Physiological Society. vol 75. issue 4. 2024-10-17. PMID:39415523. |
cell viability, apoptosis and intracellular reactive oxygen species (ros) production were assessed by mtt assay and fluorescence microscopy. |
2024-10-17 |
2024-10-19 |
human |
| Jacopo Manso, Simona Censi, Maria Chiara Pedron, Loris Bertazza, Alberto Mondin, Edoardo Ruggeri, Susi Barollo, Chiara Sabbadin, Isabella Merante Boschin, Decio Armanini, Caterina Mia. Anti-Proliferative and Anti-Migratory Activity of Licorice Extract and Glycyrrhetinic Acid on Papillary Thyroid Cancer Cell Cultures. International journal of molecular sciences. vol 25. issue 19. 2024-10-16. PMID:39409128. |
we used the mtt test to investigate the anti-proliferative activity, the wound healing test for the migratory activity, and finally, we analyzed cell cycle distribution, apoptosis, and oxidative stress after le, ga, or aldosterone incubation. |
2024-10-16 |
2024-10-18 |
Not clear |
| Xuandi Wu, Jia Hong, Liang Hon. The Deubiquitinating Enzyme USP4 Promotes Trophoblast Dysfunction by Stabilizing RYBP. Cell biochemistry and biophysics. 2024-10-15. PMID:39405024. |
cell viability, proliferation, apoptosis, invasion, and migration were assessed using 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-h-tetrazolium bromide (mtt), 5-ethynyl-2'-deoxyuridine (edu), flow cytometry, transwell, and wound healing assays. |
2024-10-15 |
2024-10-17 |
Not clear |
| Fan Wu, Liqun Li, Zhigang Li, Dabiao Zhou, Zhihui Huang, Dawei Sang, Chizi Ha. Mechanism of Mettl14 regulating AIM2 inflammasome activation and neuronal apoptosis and pyroptosis in spinal cord injury by mediating PPARγ m6A methylation. Journal of physiology and biochemistry. 2024-10-14. PMID:39400644. |
the oxygen/glucose deprivation (ogd)-induced spinal cord neuronal cell models were processed with si-mettl14 and si-peroxisome proliferator-activated receptor γ (pparγ) plasmids, and pcdna3.1-ythdf2 or synthetic dsdna poly(da: dt), followed by viability and apoptosis evaluation by mtt and flow cytometry. |
2024-10-14 |
2024-10-16 |
mouse |
| Wenjun Hou, Yifan Zou, Jie Li, Hui Jiang, Jinyu Li, Jie Wu, Senlin Zhu, Yan Ding, Huae Xu, Feng Jia, Xiaolin L. Synergistic Therapy of Melanoma by Co-Delivery of Dacarbazine and Ferroptosis-Inducing Ursolic Acid Using Biomimetic Nanoparticles. ACS omega. vol 9. issue 40. 2024-10-14. PMID:39398166. |
mtt, apoptosis assay, wound healing assay, colony formation assay, and immunohistochemistry were used to assess the antitumor effect of nps and rnps. |
2024-10-14 |
2024-10-16 |
Not clear |