| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Ashish Kuma. Human papillomavirus-16 E6-positive cervical cancer attenuated by potent 2-(4-biphenylyl)-N-(1-ethyl-4-piperidinyl) acetamide second-generation analogs with improved binding affinity. Biotechnology and applied biochemistry. 2024-07-23. PMID:39039663. |
mtt (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) assay for proliferation and flow cytometry for target inhibition and apoptosis were employed. |
2024-07-23 |
2024-07-25 |
human |
| Yuan-Fang Fan, Xiao-Hui Su, Ying Xu, Xiang-Ying Kong, Li-Ling Liu, Ya-Ge Tian, Na Li. [Effect of triptolide on reproductive toxicity in female rats with Ⅱ type collagen induced arthritis]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 49. issue 11. 2024-07-23. PMID:39041166. |
in vitro, the mouse chinese hamster ovary(cho) cell line was established, and after 24 hours of tp administration(30, 60, 120 nmol·l~(-1)), cell proliferation was detected by the thiazolyl blue tetrazolium bromide(mtt) method, apoptosis by the flow cytometry, and tgfβ3, smad3 and sf-1 protein expression in cells by the western blot method, and the nuclear entry of sf-1 was detected by immunofluorescence. |
2024-07-23 |
2024-07-25 |
mouse |
| A Abulaiti, X H Sun, W Yibulayin, D He, K M Xu, X Yibulayi. Oleanolic acid conjugated chitosan nanocomplex exerts anti-tumor effects by inhibiting autophagy in lung cancer cells through the signal transducers and activators of transcription 3/B cell lymphoma-2 signaling pathway. Journal of physiology and pharmacology : an official journal of the Polish Physiological Society. vol 75. issue 3. 2024-07-23. PMID:39042392. |
cell counting kit-8 (cck-8) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (mtt) assay were used to detect cell viability, 5-ethynyl-2'-deoxyuridine (edu) assay to detect cell proliferation, flow cytometry and tunel assay to detect cell apoptosis in a549 (atcc |
2024-07-23 |
2024-07-25 |
Not clear |
| Bo Deng, Ruyu Xian, Yuan Shu, Haohan Xia, Chengcheng Fen. Paeonol attenuated high glucose-induced apoptosis via up-regulating miR-223-3p in mouse cardiac microvascular endothelial cells. Scientific reports. vol 14. issue 1. 2024-07-19. PMID:39030268. |
cell viability was assessed by mtt assay and apoptosis was quantified by flow cytometry. |
2024-07-19 |
2024-07-22 |
mouse |
| Yuting Li, Wenyan Wang, Na Liu, Kexie Wang, Fei Re. A NOVEL CIRC_SUPT3/MIR-185-5P/G3BP2 CERNA NETWORK REGULATES HIGH GLUCOSE-INDUCED INJURY IN MOUSE PODOCYTE MPC5 CELLS. Shock (Augusta, Ga.). vol 62. issue 2. 2024-07-17. PMID:38813926. |
5-ethynyl-2'-deoxyuridine (edu) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (mtt) assays were used to examine cell proliferation, and flow cytometry was used to detect cell apoptosis. |
2024-07-17 |
2024-07-19 |
mouse |
| Zeinab Mahdian, Mahdi Pouramir, Hassan Akrami, Ebrahim Zabih. Evaluation of Drug Resistance in the Tamoxifen-treated MKN-45 Gastric Cancer Cell Line via the Epithelial-mesenchymal Transition Signaling Pathway. International journal of molecular and cellular medicine. vol 12. issue 4. 2024-07-16. PMID:39006197. |
the mtt assay was used to investigate the cytotoxic effects of 5-fu and tam, and the dna laddering technique was used to assess dna fragmentation and apoptosis. |
2024-07-16 |
2024-07-18 |
human |
| Sheyda Jodeiry Zaer, Mahmoudreza Aghamaali, Souzan Najafi, Seyed Samad Hosseini, Mohammad Amini, Mohammad Amin Doustvandi, Nazila Mozammel, Behzad Baradaran, Amir Ali Mokhtarzade. MicroRNA-143 overexpression enhances the chemosensitivity of A172 glioblastoma cells to carmustine. Naunyn-Schmiedeberg's archives of pharmacology. 2024-07-15. PMID:39007927. |
to assess the cell viability, apoptosis induction, and cell cycle progression, the mtt assay, annexin v/pi apoptosis assay, and flow cytometry were used, respectively. |
2024-07-15 |
2024-07-18 |
human |
| Fang-Fang Zhong, Bo Wei, Guo-Xiang Bao, Yi-Ping Lou, Ming-Er Wei, Xin-Yue Wang, Xiao Xiao, Jin-Jin Tia. FABP3 Induces Mitochondrial Autophagy to Promote Neuronal Cell Apoptosis in Brain Ischemia-Reperfusion Injury. Neurotoxicity research. vol 42. issue 4. 2024-07-15. PMID:39008165. |
cell viability, apoptosis rate, mitochondrial oxidative stress, morphology, autophagosome formation, membrane potential, lc3 protein levels, and colocalization of autophagosomes and mitochondria were evaluated using mtt assay, ldh release assay, flow cytometry, ros/mda/gsh-px measurement, transmission electron microscopy, mitotracker staining, jc-1 method, western blot, and immunofluorescence staining. |
2024-07-15 |
2024-07-18 |
mouse |
| Wei Wang, Luni Tan, Liang Ge, Ruiqiang Gou, Li Gou, Lin Liu, Lili Zhang, Xiaoling M. Circ_0043314 modulates proliferation and apoptosis of ovarian granulosa cells in polycystic ovarian syndrome via the microRNA-146b-3p/Apelin 13 axis. Gynecologic and obstetric investigation. 2024-07-15. PMID:39008951. |
ovarian granulosa cells were transfected with corresponding plasmids to clarify the influence of circ_0043314, mir-146b-3p, or apelin 13 on proliferation and apoptosis of ovarian granulosa cells through mtt and flow cytometry assays. |
2024-07-15 |
2024-07-18 |
human |
| Qais Ibrahee. The Role of Matrix Metalloproteinase-2 (MMP2) in Colorectal Cancer Progression: Correlation With Clinicopathological Features and Impact on Cellular Processes. Cureus. vol 16. issue 6. 2024-07-10. PMID:38978899. |
the human crc cell line sw480 was treated with an mmp2 inhibitor, arp100, and evaluated for changes in cell migration, invasion, proliferation, and apoptosis using various assays, including mtt, wound-healing, transwell, caspase activity, and western blot analysis. |
2024-07-10 |
2024-07-12 |
human |
| Bo Peng, Quanhong Dai, Xiaodong Liu, Songyang Jian. Fraxin alleviates oral lichen planus by suppressing OCT3-mediated activation of FGF2/NF-κB pathway. Naunyn-Schmiedeberg's archives of pharmacology. 2024-07-09. PMID:38980409. |
fraxin, ad-oct3, sh-oct3, and sh-fgf2 were, respectively, applied to treat lps-incubated hacat cells, and cell viability, apoptosis, and secretion of inflammatory factors were detected with mtt, flow cytometry, and elisa assays. |
2024-07-09 |
2024-07-12 |
human |
| Xu Jiang, Tan Zhijian, Cao Min, Yu Rong, Tan Xinghui, Xin Gon. Basic study on cryopreservation of rat calvarial osteoblasts with different cryoprotectants. Cell and tissue banking. 2024-07-08. PMID:38976150. |
the viability, proliferation, apoptosis, and osteogenic activity of osteoblasts were assessed by trypan blue staining, mtt, flow cytometry, and alkaline phosphatase (alp) staining. |
2024-07-08 |
2024-07-11 |
rat |
| Chongwen Ren, Qingqing Tu, Jinchao H. Fabrication of pH-responsive temozolomide (TMZ)-clacked tannic acid-altered zeolite imidazole nanoframeworks (ZIF-8) enhance anticancer activity and apoptosis induction in glioma cancer cells. Journal of biomaterials science. Polymer edition. 2024-07-02. PMID:38953298. |
the effectiveness of tmz@ta@zif-8 nps in inhibiting the migration and invasion of u251 glioma cancer cells, as well as promoting apoptosis, was confirmed through various tests, including mtt (3-(4,5)-dimethylthiahiazo(-z-y1)) assay, dapi/pi dual staining, and cell scratch assay. |
2024-07-02 |
2024-07-06 |
Not clear |
| Lei Liu, Shaik Althaf Hussain, Xiaoyan H. Fisetin reduces the resistance of MOLT-4 and K562 cells to TRAIL-induced apoptosis through upregulation of TRAIL receptors. Naunyn-Schmiedeberg's archives of pharmacology. 2024-06-25. PMID:38918236. |
the molt-4 and k-562 cells were treated with increasing concentrations of fisetin and its impact on the growth inhibition and apoptosis induction of trail were evaluated by mtt and annexin v/7-aad assays. |
2024-06-25 |
2024-06-29 |
Not clear |
| Ruoyu Wang, Dan Tang, Longyun Ou, Jiacheng Jiang, Yu-Nan Wu, Xuefei Tia. β-Sitosterol alleviates the malignant phenotype of hepatocellular carcinoma cells via inhibiting GSK3B expression. Human cell. vol 37. issue 4. 2024-06-23. PMID:38814517. |
cell viability and proliferation were assessed using mtt, cck-8, colony formation, and edu assays.flow cytometry was employed to evaluate cell cycle and apoptosis. |
2024-06-23 |
2024-06-25 |
human |
| Abdullah Alhazmi, Laura E Sidney, Andy Hopkinson, Hany M Elsheikh. Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells. Acta tropica. 2024-06-20. PMID:38901524. |
mtt, alamarblue and sulforhodamine b (srb), cytotoxicity assays (ldh) were used to assess the impact of accm on cell viability, proliferation and apoptosis. |
2024-06-20 |
2024-06-23 |
human |
| Rui-Tao Wang, Hao-En Liu, Hui-Yuan Su. In vitro modulation the Notch pathway by piperine: A therapeutic strategy for docetaxel-resistant and non-resistant prostate cancer. Chemical biology & drug design. vol 103. issue 6. 2024-06-19. PMID:38898371. |
through a series of assays, including mtt for cell viability, flow cytometry for apoptosis, transwell for cell migration and invasion, and western blot for protein expression analysis, we assessed the effects of piperine on these cellular functions and on the notch signaling pathway components. |
2024-06-19 |
2024-06-22 |
Not clear |
| Mozhgan Amirpour, Bentolhoda Kuhestani-Dehaghi, Setare Kheyrandish, Laya Khodayi Hajipirloo, Zahra Khaffafpour, Fatemeh Keshavarz, Mehdi Allahbakhshian-Farsan. The impact of exosomes derived from B-cell acute lymphoblastic leukemia as a growth factor on bone marrow mesenchymal stromal cells. Molecular biology reports. vol 51. issue 1. 2024-06-14. PMID:38874800. |
then mtt analysis and flowcytometry (apoptosis and cell cycle assay) besides gene expressions were employed to determine the state of msc proliferations. |
2024-06-14 |
2024-06-16 |
Not clear |
| Mozhgan Amirpour, Bentolhoda Kuhestani-Dehaghi, Setare Kheyrandish, Laya Khodayi Hajipirloo, Zahra Khaffafpour, Fatemeh Keshavarz, Mehdi Allahbakhshian-Farsan. The impact of exosomes derived from B-cell acute lymphoblastic leukemia as a growth factor on bone marrow mesenchymal stromal cells. Molecular biology reports. vol 51. issue 1. 2024-06-14. PMID:38874800. |
analysis of mtt, flow cytometry (cell cycle and apoptosis assay), and rt-qpcr showed that in the dose of 50 µg/ml the proliferation of mscs was increased significantly (p-value < 0.05). |
2024-06-14 |
2024-06-16 |
Not clear |
| Jiaqun Du, Xiaobang Liu, Junpeng Sun, Qian Wu, Yu Hu, Huan Shi, Li Zheng, Ying Liu, Chao Wu, Yu Ga. Trastuzumab-functionalized bionic pyrotinib liposomes for targeted therapy of HER2-positive breast cancer. Breast cancer research : BCR. vol 26. issue 1. 2024-06-13. PMID:38867302. |
the results of cell experiments such as mtt, flow cytometry, immunofluorescence staining and in vivo antitumor experiments showed that ptb-m-lip-her could significantly promote apoptosis and inhibit the proliferation of sk-br-3 cells. |
2024-06-13 |
2024-06-15 |
Not clear |