| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Aizhen Li, Mingjin Yang, Wenbiao Duan, Bo W. Dihydrotanshinone I induces necroptosis and cell cycle arrest in gastric cancer through the PTPN11/p38 pathway. Toxicology in vitro : an international journal published in association with BIBRA. 2024-10-20. PMID:39427814. |
in this study, mtt assays, apoptosis detection, immunofluorescence, and functional studies were used to elucidate the mechanisms underlying the effects of dihydrotanshinone i (dht) on gastric cancer cells. |
2024-10-20 |
2024-10-23 |
Not clear |
| Janmejay Pant, Lovedeep Singh, Payal Mittal, Nitish Kuma. Valencene as a novel potential downregulator of THRB in NSCLC: network pharmacology, molecular docking, molecular dynamics simulation, ADMET analysis, and in vitro analysis. Molecular diversity. 2024-10-19. PMID:39425858. |
in vitro assays measured cell viability in a549 and hel 299 cells via mtt assays, assessed apoptosis through hoechst staining, and evaluated mitochondrial potential with jc-1. |
2024-10-19 |
2024-10-22 |
Not clear |
| K Bontor, B Gabrye. Sulodexide protects endothelial cells against 4-hydroxynonenal-induced oxidative stress and glutathione-dependent redox imbalance by modulation of sestrin2/nuclear factor erythroid 2-related factor 2 pathway. Journal of physiology and pharmacology : an official journal of the Polish Physiological Society. vol 75. issue 4. 2024-10-17. PMID:39415523. |
cell viability, apoptosis and intracellular reactive oxygen species (ros) production were assessed by mtt assay and fluorescence microscopy. |
2024-10-17 |
2024-10-19 |
human |
| Jacopo Manso, Simona Censi, Maria Chiara Pedron, Loris Bertazza, Alberto Mondin, Edoardo Ruggeri, Susi Barollo, Chiara Sabbadin, Isabella Merante Boschin, Decio Armanini, Caterina Mia. Anti-Proliferative and Anti-Migratory Activity of Licorice Extract and Glycyrrhetinic Acid on Papillary Thyroid Cancer Cell Cultures. International journal of molecular sciences. vol 25. issue 19. 2024-10-16. PMID:39409128. |
we used the mtt test to investigate the anti-proliferative activity, the wound healing test for the migratory activity, and finally, we analyzed cell cycle distribution, apoptosis, and oxidative stress after le, ga, or aldosterone incubation. |
2024-10-16 |
2024-10-18 |
Not clear |
| Xuandi Wu, Jia Hong, Liang Hon. The Deubiquitinating Enzyme USP4 Promotes Trophoblast Dysfunction by Stabilizing RYBP. Cell biochemistry and biophysics. 2024-10-15. PMID:39405024. |
cell viability, proliferation, apoptosis, invasion, and migration were assessed using 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-h-tetrazolium bromide (mtt), 5-ethynyl-2'-deoxyuridine (edu), flow cytometry, transwell, and wound healing assays. |
2024-10-15 |
2024-10-17 |
Not clear |
| Fan Wu, Liqun Li, Zhigang Li, Dabiao Zhou, Zhihui Huang, Dawei Sang, Chizi Ha. Mechanism of Mettl14 regulating AIM2 inflammasome activation and neuronal apoptosis and pyroptosis in spinal cord injury by mediating PPARγ m6A methylation. Journal of physiology and biochemistry. 2024-10-14. PMID:39400644. |
the oxygen/glucose deprivation (ogd)-induced spinal cord neuronal cell models were processed with si-mettl14 and si-peroxisome proliferator-activated receptor γ (pparγ) plasmids, and pcdna3.1-ythdf2 or synthetic dsdna poly(da: dt), followed by viability and apoptosis evaluation by mtt and flow cytometry. |
2024-10-14 |
2024-10-16 |
mouse |
| Wenjun Hou, Yifan Zou, Jie Li, Hui Jiang, Jinyu Li, Jie Wu, Senlin Zhu, Yan Ding, Huae Xu, Feng Jia, Xiaolin L. Synergistic Therapy of Melanoma by Co-Delivery of Dacarbazine and Ferroptosis-Inducing Ursolic Acid Using Biomimetic Nanoparticles. ACS omega. vol 9. issue 40. 2024-10-14. PMID:39398166. |
mtt, apoptosis assay, wound healing assay, colony formation assay, and immunohistochemistry were used to assess the antitumor effect of nps and rnps. |
2024-10-14 |
2024-10-16 |
Not clear |
| Jin-Jin Zhao, Jie Zhao, Fei Lin, Li-Li Xu, Zhi-Gang Chen, Yu-Qin Jiang, Guo-An Zha. A quinoline-2-thione derivative as a novel chemotherapy drug candidate displays anti-tumor activity in vitro and in vivo. BMC cancer. vol 24. issue 1. 2024-10-13. PMID:39397012. |
the effect of ka3d on the viability of ovarian cancer cells was evaluated using mtt assay, and its effects on apoptosis and the cell cycle were detected using flow cytometry. |
2024-10-13 |
2024-10-16 |
mouse |
| Mona Khoshbakht, Mohammad Mahdi Forghanifard, Hossein Aghamollaei, Jafar Aman. In silico and in vitro evaluation of a PE38 and Nb-based recombinant immunotoxin targeting the GRP78 receptor in cancer cells. Biotechnology and applied biochemistry. 2024-10-13. PMID:39397264. |
accordingly, the cytotoxicity, binding affinity, cell internalization, and apoptosis were evaluated using mtt, enzyme-linked immunosorbent assay, and western blot. |
2024-10-13 |
2024-10-16 |
Not clear |
| Soumya Subhashree Satapathy, Ruchi Bhuyan, Arun Kumar Pradhan, Soumya Satpathy, Nihar Ranjan Panda, Sanat Kumar Bhuya. Comparative evaluation of anticancer potential of Spilanthes paniculata leaf and flower essential oil using annexin V and orosphere formation in oral cancer cell line. Medical oncology (Northwood, London, England). vol 41. issue 11. 2024-10-05. PMID:39369172. |
the anticancer activity was performed through an mtt assay, apoptosis assays using annexin v and orospheres formation assays. |
2024-10-05 |
2024-10-08 |
Not clear |
| Yaqi Li, Yixuan Chen, Peng Yu, Deju Zhang, Xiaoyi Tang, Zicheng Zhu, Fan Xiao, Wei Deng, Yang Liu, Zhaoying Tan, Jing Zhang, Shuchun Y. Mild therapeutic hypothermic protection activates the PI3K/AKT signaling pathway to inhibit TRPM7 and suppress ferroptosis induced by myocardial ischemia‑reperfusion injury. Molecular medicine reports. vol 30. issue 6. 2024-10-04. PMID:39364741. |
the mtt assay was used to assess cell viability, cytotoxicity was measured using a lactate dehydrogenase cytotoxicity assay, and annexin v‑fitc/pi flow cytometric analysis was used to analyze early and late cell apoptosis. |
2024-10-04 |
2024-10-07 |
rat |
| Najibeh Shekari, Dariush Shanehbandi, Elham Baghbani, Sahar Safaei, Javad Masoumi, Behzad Baradaran, Seyed Amir Jalal. VSIG-3/IGSF11 silencing in A2058 melanoma cells simultaneously suppresses melanoma progression and induces anti-tumoral cytokine profile in human T cells: In silico and in vitro study. Naunyn-Schmiedeberg's archives of pharmacology. 2024-10-04. PMID:39365310. |
then, the effects of vsig-3/igsf11 silencing on proliferation, migration, cell cycle arrest, and apoptosis in a2058 melanoma cells were assessed using mtt, colony formation, wound healing, cell cycle, and annexin-v fitc/pi assays, respectively. |
2024-10-04 |
2024-10-07 |
human |
| Vida Shirani Asl, Hassan Rafieemehr, Gholamhossein Tamaddo. The impact of Trifolium pratense extract on apoptosis and autophagy in NALM-6 cells: implications for B-ALL intervention. Medical oncology (Northwood, London, England). vol 41. issue 11. 2024-10-01. PMID:39352436. |
the mtt assay revealed an ic50 of 231 μg/ml at 48 h, and flow cytometry analysis showed a 51.8% increase in apoptosis in this cell line. |
2024-10-01 |
2024-10-03 |
Not clear |
| Ning Xiao, Yao Huang, Qi Tang, Hong Chen, Jin Hua Xiao, Chu Yang Huang, Xiangxi Yao, Hua Sheng Zha. Anticancer effects of tanshinone IIA in bladder urothelial carcinoma by down-regulating aurora A, HIF-1α and Bcl-2 both in vitro and in vivo. Pakistan journal of pharmaceutical sciences. vol 37. issue 3. 2024-09-28. PMID:39340854. |
the apoptosis, proliferation and invasion of t24 cells were evaluated using mtt assays, annexin v-fitc staining, hoechst staining and trans well assay. |
2024-09-28 |
2024-10-01 |
mouse |
| Yang Su, Yan Du, Wenguang H. USP1-mediated deubiquitination of KDM1A promotes the malignant progression of triple-negative breast cancer. Journal of biochemical and molecular toxicology. vol 38. issue 10. 2024-09-25. PMID:39318028. |
cell proliferation, apoptosis, invasion, migration and stemness were evaluated by mtt assay, edu assay, flow cytometry, transwell invasion assay, wound-healing assay and sphere-formation assay, respectively. |
2024-09-25 |
2024-09-27 |
mouse |
| Richard Göttlich, Mats Georg, Anton A Legin, Michaela Hejl, Michael A Jakupec, Jonathan Becke. Synthesis and antiproliferative activity of cisplatin-3-chloropiperidine conjugates. Chembiochem : a European journal of chemical biology. 2024-09-20. PMID:39301577. |
moreover, both complexes were able to largely circumvent the acquired cisplatin resistance of a2780cisr ovarian cancer cells, both in the mtt assay and a flow-cytometric apoptosis assay. |
2024-09-20 |
2024-09-22 |
human |
| Gideon A Gyebi, Saheed O Afolabi, Oludare M Ogunyemi, Ibrahim M Ibrahim, Olufunke E Olorundare, Joseph O Adebayo, Mamoru Kokets. Apoptotic Potential of Iloneoside from Gongronema latifolium Benth against Prostate Cancer Cells Using In Vitro and In Silico Approach. Cell biochemistry and biophysics. 2024-09-20. PMID:39302620. |
herein, we investigated the growth inhibitory effect of the gongronema latilolium leaf methanol extract and isolated pregnane (iloneoside) against prostate cancer cell lines using the mtt cell proliferation assay, apoptosis quantification, cell cycle analysis using flow cytometry and computational analysis molecular docking, molecular dynamics simulation (mds), binding free energy computation and cluster analysis. |
2024-09-20 |
2024-09-22 |
Not clear |
| Jing-Rui Song, Zhen-Peng Niu, Kun Yang, Li Wang, Yu-Bing Huang, Qing Rao, Hai-Yang Liu, Xiao-Jiang Hao, Yan-Mei L. A natural acylphloroglucinol exerts anti-erythroleukemia effects via targeting STAT3 and p38-MAPK, and inhibiting PI3K/AKT/mTOR signaling pathway. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 180. 2024-09-20. PMID:39303451. |
mtt assays were performed to evaluate the inhibitory effects of l76 on cancer cell viability, while flow cytometry was used to analyze apoptosis and cell cycle arrest in hel cells. |
2024-09-20 |
2024-09-23 |
human |
| Negar Nasri, Shaghayegh Saharkhiz, Ghasem Dini, Fariba Ghasemvan. Heliyon. vol 10. issue 17. 2024-09-19. PMID:39296244. |
additionally, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) and apoptosis assays, as well as fluorescence microscopy, were used to assess the performance of the fabricated system. |
2024-09-19 |
2024-09-21 |
Not clear |
| Wen Zhang, Shuhui Cai, Wenhao Luan, Menglei Ding, Liuqing D. Integrated serum pharmacochemistry, network pharmacology and experimental verification to explore the mechanism of Aconiti Lateralis Radix Praeparata in treatment of lung cancer. Journal of pharmaceutical and biomedical analysis. vol 252. 2024-09-15. PMID:39278160. |
afterwards, the proliferation, mitochondrial membrane potential (mmp), apoptosis and reactive oxygen species (ros) of lung cancer cells after treatment with fuzi-containing serum were determined by mtt assay, jc-1 fluorescent probe, annexin v-fitc/pi double staining and dcfh-da respectively. |
2024-09-15 |
2024-09-18 |
rat |