| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| F A Mercuri, K J Doege, E C Arner, M A Pratta, K Last, A J Fosan. Recombinant human aggrecan G1-G2 exhibits native binding properties and substrate specificity for matrix metalloproteinases and aggrecanase. The Journal of biological chemistry. vol 274. issue 45. 1999-12-13. PMID:10542281. |
the size of rg1-g2 by sds-polyacrylamide gel electorphoresis was unchanged following digestion with keratanase and keratanase ii and reduced by only 2-5 kda following digestion with either o-glycosidase or n-glycosidase f. recombinant g1-g2 was digested with purified matrix metalloproteinases (mmp), isolated aggrecanase, purified atrolysin c, or proteinases present in conditioned medium from cartilage explant cultures, and the products analyzed on sds gels by silver stain and immunoblotting. |
1999-12-13 |
2023-08-12 |
human |
| G Hunt, W Nashabe. Capillary electrophoresis sodium dodecyl sulfate nongel sieving analysis of a therapeutic recombinant monoclonal antibody: a biotechnology perspective. Analytical chemistry. vol 71. issue 13. 1999-08-23. PMID:10405607. |
the labeled sample is then incubated with sds, and the sds-protein complexes are then separated by ce using a hydrophilic polymer as a sieving matrix. |
1999-08-23 |
2023-08-12 |
Not clear |
| M J Horton, R H Getzenber. Rat seminal-vesicle secretory protein SVS II binds DNA with a preference for the 5' regulatory region of secretory protein SVS IV gene: co-isolation with components of the nuclear matrix. Journal of andrology. vol 20. issue 2. 1999-07-09. PMID:10232662. |
nuclear matrix proteins were prepared from purified nuclei with dnase and 2 m nacl, separated in sds gels, and transferred onto membranes for dna-binding (southwestern) and immunological (western) analyses. |
1999-07-09 |
2023-08-12 |
rat |
| T Kumagai, I Lee, Y Ono, M Maeno, M Takag. Ultrastructural localization and biochemical characterization of vitronectin in developing rat bone. The Histochemical journal. vol 30. issue 2. 1999-05-28. PMID:10192552. |
subsequent western blot analysis of sodium dodecyl sulphate (sds)-polyacrylamide gel electrophoresis revealed that the antibodies produced only a single band at an m(r) of approximately 73000 in both g1 and e extracts, indicating the presence of vitronectin in the mineralized bone matrix. |
1999-05-28 |
2023-08-12 |
rat |
| A R Banerjee, R James, A A Narula, R J Le. Matrix metalloproteinase-1 in cholesteatoma, middle ear granulations and deep meatal skin: a comparative analysis. Clinical otolaryngology and allied sciences. vol 23. issue 6. 1999-03-18. PMID:9884804. |
nine cholesteatomas, seven middle ear granulations and five deep meatal skin specimens were analysed for gelatinase activity at molecular weights corresponding to those of matrix metalloproteinase-1 (mmp-1) using sds page zymography. |
1999-03-18 |
2023-08-12 |
Not clear |
| A A Siddiqui, T Sultana, N P Buchholz, M A Waqar, J Talat. Proteins in renal stones and urine of stone formers. Urological research. vol 26. issue 6. 1999-03-12. PMID:9879817. |
using an extraction buffer containing sds and beta-mercaptoethanol, we were able to overcome known problems of protein isolation from urinary stone matrix. |
1999-03-12 |
2023-08-12 |
Not clear |
| T Manabe, H Oota, J Muka. Size separation of sodium dodecyl sulfate complexes of human plasma proteins by capillary electrophoresis employing linear polyacrylamide as a sieving polymer. Electrophoresis. vol 19. issue 13. 1998-12-18. PMID:9788314. |
electrophoretic conditions to separate sodium dodecyl sulfate (sds) complexes of human plasma proteins according to their size differences, by capillary electrophoresis employing linear polyacrylamide as a sieving matrix (lpa-ce), have been examined. |
1998-12-18 |
2023-08-12 |
human |
| T Watanabe, A Yamamoto, S Nagai, S Terab. Simultaneous measurement of alpha-amylase and glucoamylase activities in sake rice koji by capillary electrophoresis of sodium dodecyl sulfate-protein complexes and activity measurement of glucoamylase by in-capillary enzyme reaction method. Electrophoresis. vol 19. issue 13. 1998-12-18. PMID:9788317. |
capillary electrophoresis (ce) of sodium dodecyl sulfate (sds)-protein complexes using a nongel sieving matrix (ce-sds) has been applied to the simultaneous analysis of alpha-amylase and glucoamylase activity in sake rice koji which is employed for the brewing of sake. |
1998-12-18 |
2023-08-12 |
Not clear |
| A Ohman, P O Lycksell, A Juréus, U Langel, T Bartfai, A Gräslun. NMR study of the conformation and localization of porcine galanin in SDS micelles. Comparison with an inactive analog and a galanin receptor antagonist. Biochemistry. vol 37. issue 25. 1998-07-20. PMID:9636064. |
by using two-dimensional proton nmr spectroscopy, complete relaxation matrix analysis, and simulated annealing, the conformation of porcine galanin was determined in a membrane-mimicking solvent containing sodium dodecyl sulfate (sds) micelles. |
1998-07-20 |
2023-08-12 |
Not clear |
| K Ishii, N Tsutsui, T Watanabe, T Yanagisawa, H Nagasaw. Solubilization and chemical characterization of an insoluble matrix protein in the gastroliths of a crayfish, Procambarus clarkii. Bioscience, biotechnology, and biochemistry. vol 62. issue 2. 1998-05-21. PMID:9532785. |
an insoluble matrix protein, referred to as gastrolith matrix protein, was made soluble with 1% sds containing 10 mm dithiothreitol, and was purified by reverse-phase high-performance liquid chromatography. |
1998-05-21 |
2023-08-12 |
Not clear |
| L K Shao, D C Lock. Separation of paclitaxel and related taxanes by micellar electrokinetic capillary chromatography. Analytical chemistry. vol 70. issue 5. 1998-04-16. PMID:9511466. |
an aqueous acetonitrile (acn) buffer containing sodium dodecyl sulfate (sds) surfactant allows resolution of the 15 taxanes from each other and from the principal matrix ingredient in the injectable dosage form of the drug, cremophor el (polyethoxylated castor oil). |
1998-04-16 |
2023-08-12 |
Not clear |
| J Jarvet, J Zdunek, P Damberg, A Gräslun. Three-dimensional structure and position of porcine motilin in sodium dodecyl sulfate micelles determined by 1H NMR. Biochemistry. vol 36. issue 26. 1997-07-21. PMID:9201964. |
the solution structure of the porcine gastrointestinal peptide hormone motilin was determined in the presence of sodium dodecyl sulfate (sds) micelles at 28 degrees c using 1h nuclear magnetic resonance, full relaxation matrix analysis, and structure calculations based on restrained molecular dynamics. |
1997-07-21 |
2023-08-12 |
Not clear |
| M Nakatani, A Shibukawa, T Nakagaw. Separation mechanism of pullulan solution-filled capillary electrophoresis of sodium dodecyl sulfate-proteins. Electrophoresis. vol 17. issue 10. 1997-03-11. PMID:8957185. |
the separation mechanism of capillary electrophoresis of sodium dodecyl sulfate (sds)-proteins using pullulan with a molecular mass range of 50,000-100,000 as a separation matrix was investigated. |
1997-03-11 |
2023-08-12 |
Not clear |
| C Eckerskorn, R Grim. Enhanced in situ gel digestion of electrophoretically separated proteins with automated peptide elution onto mini reversed-phase columns. Electrophoresis. vol 17. issue 5. 1996-12-05. PMID:8783015. |
to enhance the sensitivity of the method, and to reduce the amount of sample handling steps, we have automated the extraction procedure of peptides after protein cleavage in a sodium dodecyl sulfate (sds) gel matrix. |
1996-12-05 |
2023-08-12 |
Not clear |
| M A Winkler, S Kundu, T E Robey, W G Robe. Comparative peptide mapping of a hepatitis C viral recombinant protein by capillary electrophoresis and matrix-assisted laser desorption time-of-flight mass spectrometry. Journal of chromatography. A. vol 744. issue 1-2. 1996-12-02. PMID:8843666. |
capillary electrophoresis (ce) and matrix assisted laser desorption time-of-flight mass spectrometry (maldi-tof-ms) were investigated as alternatives to sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis for peptide mapping with staphylococcus aureus protease (v8) of a hydrophobic recombinant hepatitis c virus antigen, hc-31, which required 0.1% sds for solubility. |
1996-12-02 |
2023-08-12 |
Not clear |
| A Ghosh, L Kole, K Bandyopadhyay, K Sarkar, P K Da. Evidence of a laminin binding protein on the surface of Leishmania donovani. Biochemical and biophysical research communications. vol 226. issue 1. 1996-10-22. PMID:8806598. |
the same protein was obtained when parasite outer membrane proteins were adsorbed to laminin-sepharose affinity matrix and subsequently eluted with sds. |
1996-10-22 |
2023-08-12 |
Not clear |
| X Liang, J Bai, Y H Liu, D M Lubma. Characterization of SDS--PAGE-separated proteins by matrix-assisted laser desorption/ionization mass spectrometry. Analytical chemistry. vol 68. issue 6. 1996-07-19. PMID:8651486. |
the proteins, electroblotted onto nitrocellulose after sds--page separation and stained with reversible ponceau s dye, are readily recovered by dissolving the membrane in matrix solutions prepared with acetone. |
1996-07-19 |
2023-08-12 |
cattle |
| H Chen, D F Moshe. Formation of sodium dodecyl sulfate-stable fibronectin multimers. Failure to detect products of thiol-disulfide exchange in cyanogen bromide or limited acid digests of stabilized matrix fibronectin. The Journal of biological chemistry. vol 271. issue 15. 1996-06-20. PMID:8621558. |
matrix fibronectin associates as large complexes in sds unless a reducing reagent is also present. |
1996-06-20 |
2023-08-12 |
Not clear |
| H Chen, D F Moshe. Formation of sodium dodecyl sulfate-stable fibronectin multimers. Failure to detect products of thiol-disulfide exchange in cyanogen bromide or limited acid digests of stabilized matrix fibronectin. The Journal of biological chemistry. vol 271. issue 15. 1996-06-20. PMID:8621558. |
when cyanogen bromide digests of matrix 125i-fibronectin and protomeric 125i-fibronectin labeled with bolton-hunter reagent were analyzed by two-dimensional polyacrylamide gel electrophoresis in sds, with the first dimension being nonreducing and the second reducing, we were not able to identify any fragments of matrix fibronectin that migrated as high molecular weight complexes in the first dimension. |
1996-06-20 |
2023-08-12 |
Not clear |
| P D Donatien, S J Orlo. Interaction of melanosomal proteins with melanin. European journal of biochemistry. vol 232. issue 1. 1995-11-14. PMID:7556145. |
upon incubation of a melanosome-rich fraction with the melanin precursor l-3,4-dihydroxyphenylalanine (dopa) followed by immunoblot analysis, the si locus protein, the p locus protein, and other putative matrix constituents became rapidly insoluble in sds when compared with the members of the tyrosinase-related family of melanosomal membrane proteins. |
1995-11-14 |
2023-08-12 |
Not clear |