Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
H Tokumaru, K Anzai, T Abe, Y Kirin. Purification of the cardiac 1,4-dihydropyridine receptor using immunoaffinity chromatography with a monoclonal antibody against the alpha 2 delta subunit of the skeletal muscle dihydropyridine receptor. European journal of pharmacology. vol 227. issue 4. 1992-12-30. PMID:1332871. |
purification of the cardiac 1,4-dihydropyridine receptor using immunoaffinity chromatography with a monoclonal antibody against the alpha 2 delta subunit of the skeletal muscle dihydropyridine receptor. |
1992-12-30 |
2023-08-11 |
Not clear |
H Tokumaru, K Anzai, T Abe, Y Kirin. Purification of the cardiac 1,4-dihydropyridine receptor using immunoaffinity chromatography with a monoclonal antibody against the alpha 2 delta subunit of the skeletal muscle dihydropyridine receptor. European journal of pharmacology. vol 227. issue 4. 1992-12-30. PMID:1332871. |
8 h) two-step procedure: wheat germ agglutinin affinity chromatography followed by immunoaffinity chromatography with a monoclonal antibody (mcc-1) against the alpha 2 delta subunit of the skeletal muscle ca2+ channel with a glycine elution buffer (ph 3). |
1992-12-30 |
2023-08-11 |
Not clear |
H Tokumaru, K Anzai, T Abe, Y Kirin. Purification of the cardiac 1,4-dihydropyridine receptor using immunoaffinity chromatography with a monoclonal antibody against the alpha 2 delta subunit of the skeletal muscle dihydropyridine receptor. European journal of pharmacology. vol 227. issue 4. 1992-12-30. PMID:1332871. |
gel electrophoresis of this purified sample under non-reducing conditions revealed a major polypeptide band with molecular weight of 190 kda, which was separated under reducing conditions to a 155 kda band and 2-3 bands with m(r) about 20 kda, corresponding to alpha 2 and delta subunits, respectively. |
1992-12-30 |
2023-08-11 |
Not clear |
H Tokumaru, K Anzai, T Abe, Y Kirin. Purification of the cardiac 1,4-dihydropyridine receptor using immunoaffinity chromatography with a monoclonal antibody against the alpha 2 delta subunit of the skeletal muscle dihydropyridine receptor. European journal of pharmacology. vol 227. issue 4. 1992-12-30. PMID:1332871. |
however, the alpha 1 subunit without bound alpha 2 delta was selectively eluted from mcc-1 sepharose with 1% triton x-100. |
1992-12-30 |
2023-08-11 |
Not clear |
B Y Wu, E W Hanley, L A Turka, G J Nabe. Isolation of a cDNA clone encoding a zinc finger protein highly expressed in T-leukemia lines. Blood. vol 80. issue 10. 1992-12-22. PMID:1421376. |
within the t lineage, the mrna is found at high levels in both alpha beta and gamma delta t cells. |
1992-12-22 |
2023-08-11 |
drosophila_melanogaster |
L M Amzel, M A Bianchet, P L Pederse. Quaternary structure of ATP synthases: symmetry and asymmetry in the F1 moiety. Journal of bioenergetics and biomembranes. vol 24. issue 5. 1992-12-22. PMID:1429535. |
f1-atpases (stoichiometry alpha 3 beta 3 gamma delta epsilon) are essentially a symmetrical trimer of pairs of the major subunits (alpha and beta); the minor subunits (gamma, delta and epsilon) are in single copies and interact with the trimer in an asymmetrical fashion. |
1992-12-22 |
2023-08-11 |
Not clear |
Y Kagawa, S Ohta, M Harada, H Kihara, Y Ito, M Sat. The alpha beta complexes of ATP synthase: the alpha 3 beta 3 oligomer and alpha 1 beta 1 protomer. Journal of bioenergetics and biomembranes. vol 24. issue 5. 1992-12-22. PMID:1429537. |
the basic structures of the catalytic portion (f1, alpha 3 beta 3 gamma delta epsilon) of atp synthase are the alpha 3 beta 3 hexamer (oligomer with cooperativity) and alpha 1 beta 1 heterodimer (protomer). |
1992-12-22 |
2023-08-11 |
Not clear |
G D Taylor, J A Fee, D F Silbert, S L Hofman. PI-specific phospholipase C "alpha" from sheep seminal vesicles is a proteolytic fragment of PI-PLC delta. Biochemical and biophysical research communications. vol 188. issue 3. 1992-12-22. PMID:1445352. |
pi-specific phospholipase c "alpha" from sheep seminal vesicles is a proteolytic fragment of pi-plc delta. |
1992-12-22 |
2023-08-11 |
cattle |
G D Taylor, J A Fee, D F Silbert, S L Hofman. PI-specific phospholipase C "alpha" from sheep seminal vesicles is a proteolytic fragment of PI-PLC delta. Biochemical and biophysical research communications. vol 188. issue 3. 1992-12-22. PMID:1445352. |
in the current paper we show by immunoblotting experiments that plc "alpha" from sheep seminal vesicles is recognized by monoclonal antibodies raised against the delta 1 isoform of bovine brain plc, and appears to be derived from a higher molecular weight band at 85 kda. |
1992-12-22 |
2023-08-11 |
cattle |
G D Taylor, J A Fee, D F Silbert, S L Hofman. PI-specific phospholipase C "alpha" from sheep seminal vesicles is a proteolytic fragment of PI-PLC delta. Biochemical and biophysical research communications. vol 188. issue 3. 1992-12-22. PMID:1445352. |
we also show that antibodies raised against plc alpha efficiently immunoprecipitate the 85-kda plc delta 1 isoform from bovine brain and chinese hamster lung fibroblasts. |
1992-12-22 |
2023-08-11 |
cattle |
G D Taylor, J A Fee, D F Silbert, S L Hofman. PI-specific phospholipase C "alpha" from sheep seminal vesicles is a proteolytic fragment of PI-PLC delta. Biochemical and biophysical research communications. vol 188. issue 3. 1992-12-22. PMID:1445352. |
these data provide strong evidence that the plc alpha from sheep seminal vesicles is a proteolytic fragment of plc delta 1. |
1992-12-22 |
2023-08-11 |
cattle |
W Ptak, P W Askenas. Gamma delta T cells assist alpha beta T cells in adoptive transfer of contact sensitivity. Journal of immunology (Baltimore, Md. : 1950). vol 149. issue 11. 1992-12-21. PMID:1431120. |
gamma delta t cells assist alpha beta t cells in adoptive transfer of contact sensitivity. |
1992-12-21 |
2023-08-11 |
mouse |
W Ptak, P W Askenas. Gamma delta T cells assist alpha beta T cells in adoptive transfer of contact sensitivity. Journal of immunology (Baltimore, Md. : 1950). vol 149. issue 11. 1992-12-21. PMID:1431120. |
in vitro treatment of a mixture of cs effector cells with hamster mab to gamma delta tcr, together with rabbit complement, or by panning on anti-hamster ig-coated dishes, diminished substantially the subsequent transfer of cs reactivity without affecting either cs-initiating cells, or the later-acting, alpha beta tcr+ cs effector t cells. |
1992-12-21 |
2023-08-11 |
mouse |
W Ptak, P W Askenas. Gamma delta T cells assist alpha beta T cells in adoptive transfer of contact sensitivity. Journal of immunology (Baltimore, Md. : 1950). vol 149. issue 11. 1992-12-21. PMID:1431120. |
immune cells treated with anti-alpha beta tcr mab, or recovered as adherent cells from petri dishes after anti-gamma delta tcr panning (i.e., gamma delta tcr-enriched cells), reconstituted the ability of anti-gamma delta tcr-treated immune cells (i.e., alpha beta tcr-enriched cells) to transfer 24-h cs responsiveness. |
1992-12-21 |
2023-08-11 |
mouse |
W Ptak, P W Askenas. Gamma delta T cells assist alpha beta T cells in adoptive transfer of contact sensitivity. Journal of immunology (Baltimore, Md. : 1950). vol 149. issue 11. 1992-12-21. PMID:1431120. |
the phenotype of the gamma delta t cells that assisted cs effector alpha beta t cells was: cd3+, cd4-, and cd8+. |
1992-12-21 |
2023-08-11 |
mouse |
W Ptak, P W Askenas. Gamma delta T cells assist alpha beta T cells in adoptive transfer of contact sensitivity. Journal of immunology (Baltimore, Md. : 1950). vol 149. issue 11. 1992-12-21. PMID:1431120. |
the gamma delta t cells that assisted alpha beta t cells were not ag-specific since anti-alpha beta-tcr-treated cells (gamma delta t-enriched) from picryl chloride immunized donors aided alpha beta t cells (anti-gamma delta tcr-treated) from oxazolone-immunized donors, and conversely gamma delta t cells from oxazolone-immunized donors aided alpha beta t cells from picryl chloride immunized donors. |
1992-12-21 |
2023-08-11 |
mouse |
W Ptak, P W Askenas. Gamma delta T cells assist alpha beta T cells in adoptive transfer of contact sensitivity. Journal of immunology (Baltimore, Md. : 1950). vol 149. issue 11. 1992-12-21. PMID:1431120. |
furthermore, the cs-regulating gamma delta t cells were not mhc-restricted because gamma delta t cells from h2d or h2b donors could assist alpha beta t cells from h2k donors. |
1992-12-21 |
2023-08-11 |
mouse |
W Ptak, P W Askenas. Gamma delta T cells assist alpha beta T cells in adoptive transfer of contact sensitivity. Journal of immunology (Baltimore, Md. : 1950). vol 149. issue 11. 1992-12-21. PMID:1431120. |
it was concluded that a regulatory population of non-ag specific, non-mhc-restricted gamma delta t cells was needed to assist immune effector, ag/mhc-specific alpha beta t cells in the adoptive transfer of cs. |
1992-12-21 |
2023-08-11 |
mouse |
M J Griffiths, A W Preece, J L Gree. Monitoring sedation levels by EEG spectral analysis. Anesthesia progress. vol 38. issue 6. 1992-12-21. PMID:1842161. |
overdose was indicated by an initial shift in the alpha frequency to a lower value (6-7 hz) followed by the appearance of delta waves (0.5-4 hz) and loss of alpha waves. |
1992-12-21 |
2023-08-11 |
human |
A Schwart. Molecular and cellular aspects of calcium channel antagonism. The American journal of cardiology. vol 70. issue 16. 1992-12-18. PMID:1332464. |
the l-type calcium channels comprise 5 subunits termed alpha 1, alpha 2, beta, gamma, and delta. |
1992-12-18 |
2023-08-11 |
Not clear |