All Relations between blot and oxytocin

Publication Sentence Publish Date Extraction Date Species
Deniz Önal, Hilal Korkmaz, Gizem Önal, Bilge Pehlivanoğl. Body Weight Modulates the Impact of Oxytocin on Chronic Cold-Immobilization Stress Response. Peptides. 2024-03-31. PMID:38555975. prefrontal cortex and hypothalamic oxytocin receptor (oxtr) expression levels were identified using western blot analysis. 2024-03-31 2024-04-03 rat
Jon DelaCuesta-Barrutia, Shizu Hidema, Heather K Caldwell, Katsuhiko Nishimori, Amaia M Erdozain, Olga Peñagarikan. In need of a specific antibody against the oxytocin receptor for neuropsychiatric research: A KO validation study. Journal of psychiatric research. vol 173. 2024-03-30. PMID:38554622. antibodies were first tested by western blot in brain lysates of wild-type and oxytocin receptor knockout mice. 2024-03-30 2024-04-02 mouse
Jon DelaCuesta-Barrutia, Shizu Hidema, Heather K Caldwell, Katsuhiko Nishimori, Amaia M Erdozain, Olga Peñagarikan. In need of a specific antibody against the oxytocin receptor for neuropsychiatric research: A KO validation study. Journal of psychiatric research. vol 173. 2024-03-30. PMID:38554622. in conclusion, none of the six tested commercial antibodies are suitable to detect oxytocin receptor in brain by either western blot or immunohistochemistry, although some do specifically detect it in uterus. 2024-03-30 2024-04-02 mouse
Jon DelaCuesta-Barrutia, Shizu Hidema, Heather K Caldwell, Katsuhiko Nishimori, Amaia M Erdozain, Olga Peñagarikan. In need of a specific antibody against the oxytocin receptor for neuropsychiatric research: A KO validation study. Journal of psychiatric research. vol 173. 2024-03-30. PMID:38554622. in this work, we have tested the specificity of six commercially available oxytocin receptor antibodies, indicated by the manufacturers to be suitable for western blot and with an available image showing the correct size band (45-55 kda). 2024-03-30 2024-04-02 mouse
Tomoki Kumagai, Satoru Shindo, Katsuhiro Takeda, Hideki Shib. Oxytocin suppresses CXCL10 production in TNF-α-stimulated human dental pulp stem cells. Cell biology international. 2022-07-23. PMID:35870165. expression of the oxytocin receptor (oxr) on hdpscs was detected by western blot analysis and immunofluorescence. 2022-07-23 2023-08-14 human
Mariana Diel de Amorim, Claudia Klein, Robert Foster, Lynn Dong, Maria Fernanda Lopez-Rodriguez, Claire Car. Expression of Oxytocin/Neurophysin I and Oxytocinase in the Equine Conceptus from Day 8 to Day 21 Post-Ovulation. Animals : an open access journal from MDPI. vol 12. issue 7. 2022-04-12. PMID:35405789. immunohistochemistry, western blot and liquid chromatography with tandem mass spectrometry (lc-ms/ms) were used for identification of oxytocin and lnpep in d15, 16 and 18 conceptuses. 2022-04-12 2023-08-13 Not clear
M L Gould, H D Nicholso. Changes in receptor location affect the ability of oxytocin to stimulate proliferative growth in prostate epithelial cells. Reproduction, fertility, and development. vol 31. issue 6. 2020-09-29. PMID:31034785. androgen receptor (ar) and oxytocin receptor (otr) expression was identified by immunofluorescence and quantified by western blot. 2020-09-29 2023-08-13 human
Wei Yuan, Zhixiong He, Wenjuan Hou, Limin Wang, Laifu Li, Jing Zhang, Yang Yang, Rui Jia, Hui Qiao, Fadao Ta. Role of oxytocin in the medial preoptic area (MPOA) in the modulation of paternal behavior in mandarin voles. Hormones and behavior. vol 110. 2020-01-22. PMID:30836063. both rt-pcr and western blot results indicated that the levels of the oxytocin receptor (otr) in the medial preoptic area (mpoa) of fathers were significantly higher than in virgin males, but the levels of vasopressin 1a receptor (v1ar) mrna and protein expression in the mpoa did not show significant differences. 2020-01-22 2023-08-13 Not clear
Hiroshi Miyoshi, Haruhisa Konishi, Yuko Teraoka, Satoshi Urabe, Hisako Furusho, Mutsumi Miyauchi, Takashi Takata, Yoshiki Kud. Enhanced Expression of Contractile-Associated Proteins and Ion Channels in Preterm Delivery Model Mice With Chronic Odontogenic Porphyromonas Gingivalis Infection. Reproductive sciences (Thousand Oaks, Calif.). vol 23. issue 7. 2017-10-06. PMID:26692542. we examined the expression of the oxytocin receptor, connexin 43, prostaglandin f receptors, l-type ca(2+) channel, and p2x7 receptor in the myometrium at day 18 of gestation by real-time pcr and western blot analyses. 2017-10-06 2023-08-13 mouse
Lu Yang, Zhengyu Cao, Boyang Yu, Chengzhi Cha. An in vivo mouse model of primary dysmenorrhea. Experimental animals. vol 64. issue 3. 2016-04-19. PMID:25912320. western blot analyses were performed to determine the expressions of oxytocin receptor (otr), beta2-adrenergic receptor (beta2-ar), and cyclooxygenase-2 (cox-2) in uterine, which are responsible for the uterine contraction. 2016-04-19 2023-08-13 mouse
Chihiro Miyamoto Nakazawa, Kohdoh Shikata, Mai Uesugi, Hiroyuki Katayama, Ken Aoshima, Kazuhiro Tahara, Eiki Takahashi, Takayuki Hida, Hisashi Shibata, Hiroo Ogura, Takashi Seiki, Yoshiya Oda, Junro Kuromitsu, Norimasa Miyamot. Prediction of relaxin-3-induced downstream pathway resulting in anxiolytic-like behaviors in rats based on a microarray and peptidome analysis. Journal of receptor and signal transduction research. vol 33. issue 4. 2014-03-17. PMID:23697547. one of these neuropeptides, oxytocin (oxt), exhibited increased expression in both the microarray and the peptidomic analysis, and a western blot analysis validated the mass spectrometry results. 2014-03-17 2023-08-12 rat
Shiho Wakamatsu, Hiroshi Nonoguchi, Mika Ikebe, Kenji Machida, Yuichiro Izumi, Hasiyet Memetimin, Yushi Nakayama, Takeshi Nakanishi, Yukimasa Kohda, Kimio Tomit. Vasopressin and hyperosmolality regulate NKCC1 expression in rat OMCD. Hypertension research : official journal of the Japanese Society of Hypertension. vol 32. issue 6. 2009-12-07. PMID:19390537. to determine the mechanisms by which dehydration stimulates nkcc1 expression, the effects of vasopressin, oxytocin and hyperosmolality on nkcc1 mrna and protein expressions in the outer medullary collecting duct (omcd) of rats were investigated using rt-competitive pcr and western blot analysis. 2009-12-07 2023-08-12 rat
Kate Whittington, Steve Assinder, Maree Gould, Helen Nicholso. Oxytocin, oxytocin-associated neurophysin and the oxytocin receptor in the human prostate. Cell and tissue research. vol 318. issue 2. 2005-03-08. PMID:15459766. western blot analysis detected a single band at 14 kda with neurophysin antiserum and a 66-kda band with oxytocin receptor antiserum in epithelial and stromal cell lines. 2005-03-08 2023-08-12 human
M Mlynarczyk, T Imamura, H Umezaki, K M Kaushal, L Zhang, C A Ducsa. Long-term hypoxia changes myometrial responsiveness and oxytocin receptors in the pregnant ewe: differential effects on longitudinal versus circular smooth muscle. Biology of reproduction. vol 69. issue 5. 2004-07-27. PMID:12855597. longitudinal and circular layers were separated, length-tension curves generated to determine optimal resting tension, and all strips exposed to increasing half-log doses of oxytocin ranging from 10-12 to 10-6.5 m. the expression of oxytocin-receptor protein was measured using western blot analysis. 2004-07-27 2023-08-12 Not clear
S Mukaddam-Daher, M Jankowski, D Wang, A Menaouar, J Gutkowsk. Regulation of cardiac oxytocin system and natriuretic peptide during rat gestation and postpartum. The Journal of endocrinology. vol 175. issue 1. 2002-12-10. PMID:12379505. the changes in oxytocin receptor expression at term and postpartum paralleled oxytocin receptor protein determined by western blot. 2002-12-10 2023-08-12 rat
G S Frale. Immunolesion of hindbrain catecholaminergic projections to the medial hypothalamus attenuates penile reflexive erections and alters hypothalamic peptide mRNA. Journal of neuroendocrinology. vol 14. issue 5. 2002-06-11. PMID:12000538. northern blot analyses of hypothalamic oxytocin mrna expression showed a significant increase; however, neuropeptide y mrna expression did not. 2002-06-11 2023-08-12 rat
K Whittington, S J Assinder, T Parkinson, K R Lapwood, H D Nicholso. Function and localization of oxytocin receptors in the reproductive tissue of rams. Reproduction (Cambridge, England). vol 122. issue 2. 2001-10-11. PMID:11467983. western blot analysis performed using a specific oxytocin receptor antibody (020) identified a single immunoreactive band of 66 kda in testicular and epididymal tissue. 2001-10-11 2023-08-12 Not clear
Q Chen, S S Schreiber, R D Brinto. Vasopressin and oxytocin receptor mRNA expression during rat telencephalon development. Neuropeptides. vol 34. issue 3-4. 2001-05-03. PMID:11021977. we investigated the developmental expression of vasopressin and oxytocin receptor and peptide mrna using semi-quantitative reverse transcription-polymerase chain reaction (rt-pcr) and southern blot hybridization. 2001-05-03 2023-08-12 rat
H Y Al-Matubsi, S Frazer, R J Fairclough, G Jenki. Effects of progesterone on expression of messenger RNA encoding oxytocin-neurophysin, oxytocin receptor and prostaglandin G/H synthase-1 and -2 during the early oestrous cycle in the ovine corpus luteum. Reproduction, fertility, and development. vol 11. issue 7-8. 2000-12-22. PMID:11101280. on the morning of day 4 post oestrus, the corpora lutea were collected and analysed for oxytocin-neurophysin mrna by northern blot using a labelled cdna probe, and for the expressions of the oxytocin receptor, pghs-1 and pghs-2 mrna using the reverse transcription polymerase chain reaction. 2000-12-22 2023-08-12 Not clear
S J Assinder, M Carey, T Parkinson, H D Nicholso. Oxytocin and vasopressin expression in the ovine testis and epididymis: changes with the onset of spermatogenesis. Biology of reproduction. vol 63. issue 2. 2000-10-19. PMID:10906049. localization and synthesis was investigated by immunohistochemistry and western blot analysis employing antibodies recognizing epitopes of either oxytocin, oxytocin-associated neurophysin, vasopressin, or vasopressin-associated neurophysin. 2000-10-19 2023-08-12 Not clear