| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Wenxue Wang, Shaoya Li, Jiaying Yang, Jingying Li, Lei Yan, Chen Zhang, Yubing He, Lanqin Xi. Exploiting the efficient Exo:Cas12i3-5M fusions for robust single and multiplex gene editing in rice. Journal of integrative plant biology. 2025-01-28. PMID:39873911. |
crispr/cas12i3, which belongs to the class ii type v-i cas system, has attracted extensive attention recently due to its smaller protein size and less restricted canonical "ttn" protospacer adjacent motif (pam). |
2025-01-28 |
2025-01-30 |
Not clear |
| Yunqian Zhang, Jingjing Wei, Hongyan Wang, Yongming Wan. Characterization of NiCas12b for In Vivo Genome Editing. Advanced science (Weinheim, Baden-Wurttemberg, Germany). 2024-07-30. PMID:39076074. |
particularly noteworthy is the nicas12b derived from nitrospira sp., which recognizes a "ttn" protospacer adjacent motif (pam) and facilitates efficient genome editing in various cell lines. |
2024-07-30 |
2024-08-02 |
mouse |
| Lei Yang, Guanwei Chen, Jian Wu, Wei Wei, Cheng Peng, Lin Ding, Xiaoyun Chen, Xiaoli Xu, Xiaofu Wang, Junfeng X. A PAM-Free One-Step Asymmetric RPA and CRISPR/Cas12b Combined Assay (OAR-CRISPR) for Rapid and Ultrasensitive DNA Detection. Analytical chemistry. 2024-03-29. PMID:38551977. |
furthermore, the presence of the protospacer adjacent motif (pam) motif (e.g., ttn or tttn) in the target double-strand dna (dsdna) is an essential prerequisite for the activation of the cas12-based method. |
2024-03-29 |
2024-04-01 |
Not clear |
| Yao Wang, Tao Qi, Jingtong Liu, Yuan Yang, Ziwen Wang, Ying Wang, Tianyi Wang, Miaomiao Li, Mingqing Li, Daru Lu, Alex Chia Yu Chang, Li Yang, Song Gao, Yongming Wang, Feng La. A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing. Science advances. vol 9. issue 6. 2023-02-10. PMID:36763662. |
cas12j-8 recognizes a simple ttn pam that provides for high target site density. |
2023-02-10 |
2023-08-14 |
Not clear |
| Bernd Zetsche, Omar O Abudayyeh, Jonathan S Gootenberg, David A Scott, Feng Zhan. A Survey of Genome Editing Activity for 16 Cas12a Orthologs. The Keio journal of medicine. vol 69. issue 3. 2021-07-16. PMID:31723075. |
we also showed that mb3cas12a displays some tolerance for a shortened pam (ttn versus the canonical cas12a pam tttv). |
2021-07-16 |
2023-08-13 |
Not clear |
| Hanseop Kim, Wi-Jae Lee, Yeounsun Oh, Seung-Hun Kang, Junho K Hur, Hyomin Lee, WooJeung Song, Kyung-Seob Lim, Young-Ho Park, Bong-Seok Song, Yeung Bae Jin, Bong-Hyun Jun, Cheulhee Jung, Dong-Seok Lee, Sun-Uk Kim, Seung Hwan Le. Enhancement of target specificity of CRISPR-Cas12a by using a chimeric DNA-RNA guide. Nucleic acids research. vol 48. issue 15. 2020-10-15. PMID:32687187. |
however, it tolerates mismatches in regions distant from the pam sequence (tttn or ttn) in the protospacer, and off-target cleavage issues may become more problematic when cas12a activity is improved for therapeutic purposes. |
2020-10-15 |
2023-08-13 |
Not clear |
| Bin Ren, Lang Liu, Shaofang Li, Yongjie Kuang, Jingwen Wang, Dawei Zhang, Xueping Zhou, Honghui Lin, Huanbin Zho. Cas9-NG Greatly Expands the Targeting Scope of the Genome-Editing Toolkit by Recognizing NG and Other Atypical PAMs in Rice. Molecular plant. vol 12. issue 7. 2020-03-27. PMID:30928635. |
however, the requirement of a protospacer adjacent motif (pam), such as ngg and ttn, for cas protein recognition restricts the selection of targetable genomic loci in practical applications of crispr technologies. |
2020-03-27 |
2023-08-13 |
human |
| Cristóbal Almendros, Franklin L Nobrega, Rebecca E McKenzie, Stan J J Broun. Cas4-Cas1 fusions drive efficient PAM selection and control CRISPR adaptation. Nucleic acids research. vol 47. issue 10. 2019-12-02. PMID:30937444. |
here we report that a gene fusion between cas4 and cas1 from the geobacter sulfurreducens i-u crispr-cas system is capable of introducing functional spacers carrying interference proficient ttn pam sequences at much higher frequencies than unfused cas4 adaptation modules. |
2019-12-02 |
2023-08-13 |
Not clear |
| Eszter Tóth, Bernadett C Czene, Péter I Kulcsár, Sarah L Krausz, András Tálas, Antal Nyeste, Éva Varga, Krisztina Huszár, Nóra Weinhardt, Zoltán Ligeti, Adrienn É Borsy, Elfrieda Fodor, Ervin Welke. Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic acids research. vol 46. issue 19. 2019-07-31. PMID:30239882. |
here, we show that two other cpf1 nucleases, fn- and mbcpf1, which have been reported to utilize a shorter, more frequently occurring pam sequence (ttn) when tested in vitro, carry out efficient genome modification in mammalian cells. |
2019-07-31 |
2023-08-13 |
Not clear |
| Akira Endo, Seiichi Tok. Targeted Mutagenesis Using FnCpf1 in Tobacco. Methods in molecular biology (Clifton, N.J.). vol 1917. 2019-06-17. PMID:30610643. |
cfp1 from francisella novicida (fncpf1) recognizes ttn as its protospacer adjacent motif (pam). |
2019-06-17 |
2023-08-13 |
Not clear |
| Akira Endo, Seiichi Tok. Targeted Mutagenesis Using FnCpf1 in Tobacco. Methods in molecular biology (Clifton, N.J.). vol 1917. 2019-06-17. PMID:30610643. |
ttn is a shortest pam among other known cpf1s such as ascpf1 or lbcpf1, which use tttn as pam. |
2019-06-17 |
2023-08-13 |
Not clear |
| Akira Endo, Seiichi Tok. FnCpf1-Mediated Targeted Mutagenesis in Plants. Methods in molecular biology (Clifton, N.J.). vol 1795. 2019-02-21. PMID:29846931. |
cfp1 from francisella novicida (fncpf1) recognizes ttn as its protospacer adjacent motif (pam). |
2019-02-21 |
2023-08-13 |
Not clear |
| Akira Endo, Mikami Masafumi, Hidetaka Kaya, Seiichi Tok. Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida. Scientific reports. vol 6. 2018-05-29. PMID:27905529. |
to evaluate whether cpf1 can be applied to plant genome editing, we selected cpf1 from francisella novicida (fncpf1), which recognizes a shorter pam (ttn) within known cpf1 proteins, and applied it to targeted mutagenesis in tobacco and rice. |
2018-05-29 |
2023-08-13 |
Not clear |