| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Wenxue Wang, Shaoya Li, Jiaying Yang, Jingying Li, Lei Yan, Chen Zhang, Yubing He, Lanqin Xi. Exploiting the efficient Exo:Cas12i3-5M fusions for robust single and multiplex gene editing in rice. Journal of integrative plant biology. 2025-01-28. PMID:39873911. |
crispr/cas12i3, which belongs to the class ii type v-i cas system, has attracted extensive attention recently due to its smaller protein size and less restricted canonical "ttn" protospacer adjacent motif (pam). |
2025-01-28 |
2025-01-30 |
Not clear |
| Yunqian Zhang, Jingjing Wei, Hongyan Wang, Yongming Wan. Characterization of NiCas12b for In Vivo Genome Editing. Advanced science (Weinheim, Baden-Wurttemberg, Germany). 2024-07-30. PMID:39076074. |
particularly noteworthy is the nicas12b derived from nitrospira sp., which recognizes a "ttn" protospacer adjacent motif (pam) and facilitates efficient genome editing in various cell lines. |
2024-07-30 |
2024-08-02 |
mouse |
| Lei Yang, Guanwei Chen, Jian Wu, Wei Wei, Cheng Peng, Lin Ding, Xiaoyun Chen, Xiaoli Xu, Xiaofu Wang, Junfeng X. A PAM-Free One-Step Asymmetric RPA and CRISPR/Cas12b Combined Assay (OAR-CRISPR) for Rapid and Ultrasensitive DNA Detection. Analytical chemistry. 2024-03-29. PMID:38551977. |
furthermore, the presence of the protospacer adjacent motif (pam) motif (e.g., ttn or tttn) in the target double-strand dna (dsdna) is an essential prerequisite for the activation of the cas12-based method. |
2024-03-29 |
2024-04-01 |
Not clear |
| Bin Ren, Lang Liu, Shaofang Li, Yongjie Kuang, Jingwen Wang, Dawei Zhang, Xueping Zhou, Honghui Lin, Huanbin Zho. Cas9-NG Greatly Expands the Targeting Scope of the Genome-Editing Toolkit by Recognizing NG and Other Atypical PAMs in Rice. Molecular plant. vol 12. issue 7. 2020-03-27. PMID:30928635. |
however, the requirement of a protospacer adjacent motif (pam), such as ngg and ttn, for cas protein recognition restricts the selection of targetable genomic loci in practical applications of crispr technologies. |
2020-03-27 |
2023-08-13 |
human |
| Akira Endo, Seiichi Tok. Targeted Mutagenesis Using FnCpf1 in Tobacco. Methods in molecular biology (Clifton, N.J.). vol 1917. 2019-06-17. PMID:30610643. |
cfp1 from francisella novicida (fncpf1) recognizes ttn as its protospacer adjacent motif (pam). |
2019-06-17 |
2023-08-13 |
Not clear |
| Akira Endo, Seiichi Tok. FnCpf1-Mediated Targeted Mutagenesis in Plants. Methods in molecular biology (Clifton, N.J.). vol 1795. 2019-02-21. PMID:29846931. |
cfp1 from francisella novicida (fncpf1) recognizes ttn as its protospacer adjacent motif (pam). |
2019-02-21 |
2023-08-13 |
Not clear |