All Relations between protospacer adjacent motif and pam

Publication Sentence Publish Date Extraction Date Species
Tao Hu, Quanquan Ji, Xinxin Ke, Hufeng Zhou, Senfeng Zhang, Shengsheng Ma, Chenlin Yu, Wenjun Ju, Meiling Lu, Yu Lin, Yangjing Ou, Yingsi Zhou, Yibei Xiao, Chunlong Xu, Chunyi H. Repurposing Type I-A CRISPR-Cas3 for a robust diagnosis of human papillomavirus (HPV). Communications biology. vol 7. issue 1. 2024-07-13. PMID:39003402. this type i-a crispr-cas3 system not only exhibits an expanded protospacer adjacent motif (pam) recognition capability but also demonstrates remarkable intolerance towards mismatched sequences. 2024-07-13 2024-07-16 human
Chenyu Cui, Guihuan Guo, Ting-Hsuan Che. Toehold region triggered CRISPR/Cas12a trans-cleavage for detection of uracil-DNA glycosylase activity. Biotechnology journal. vol 19. issue 7. 2024-07-10. PMID:38987221. this protospacer adjacent motif (pam)-free approach achieves remarkable sensitivity and specificity in detecting udg, with a detection limit as low as 0.000368 u ml 2024-07-10 2024-07-13 Not clear
Wenjing Li, Xuan Li, Chunyang Wang, Guanzhong Huo, Xinru Zhang, Jintai Yu, Xiaoxiao Yu, Jing Li, Chao Zhang, Jianjun Zhao, Yan Li, Jun L. Expanding the targeting scope of CRISPR/Cas9-mediated genome editing by Cas9 variants in aBIOTECH. vol 5. issue 2. 2024-07-08. PMID:38974859. however, the strict requirement for a protospacer adjacent motif (pam) has hindered the application of the crispr/cas9 system because the number of targetable genomic sites is limited. 2024-07-08 2024-07-11 Not clear
Jian Wang, Ke Wang, Zhe Deng, Zhiyu Zhong, Guo Sun, Qing Mei, Fuling Zhou, Zixin Deng, Yuhui Su. Engineered cytosine base editor enabling broad-scope and high-fidelity gene editing in Streptomyces. Nature communications. vol 15. issue 1. 2024-07-06. PMID:38971862. base editing (be) faces protospacer adjacent motif (pam) constraints and off-target effects in both eukaryotes and prokaryotes. 2024-07-06 2024-07-11 Not clear
Wei Qin, Fang Liang, Sheng-Jia Lin, Cassidy Petree, Kevin Huang, Yu Zhang, Lin Li, Pratishtha Varshney, Philippe Mourrain, Yanmei Liu, Gaurav K Varshne. ABE-ultramax for high-efficiency biallelic adenine base editing in zebrafish. Nature communications. vol 15. issue 1. 2024-07-04. PMID:38965236. however, base editors are restricted by protospacer adjacent motif (pam) sequences and specific editing windows, hindering their applicability to a broad spectrum of genetic variants. 2024-07-04 2024-07-08 zebrafish
Yao Yao, Zhiwei Zhou, Xiaoling Wang, Zhirui Liu, Yixin Zhai, Xiaolin Chi, Jingyi Du, Liheng Luo, Zhigang Zhao, Xiaoyue Wang, Chaoyou Xue, Shuquan Ra. SpRY-mediated screens facilitate functional dissection of non-coding sequences at single-base resolution. Cell genomics. 2024-06-18. PMID:38889719. crispr mutagenesis screens conducted with spcas9 and other nucleases have identified certain cis-regulatory elements and genetic variants but at a limited resolution due to the absence of protospacer adjacent motif (pam) sequences. 2024-06-18 2024-06-21 Not clear
Hyogu Han, Se Hee Jang, Jun Ki Ahn, Chang Yeol Le. DNA repair-retarded isothermal CRISPR amplification for rapid, sensitive base excision repair enzyme assay. Analytica chimica acta. vol 1314. 2024-06-14. PMID:38876521. after isothermal amplification, a crispr/cas reagent should be separately added with extra manual steps and its guide rna (grna) should be designed, considering the presence of protospacer adjacent motif (pam) site. 2024-06-14 2024-06-17 Not clear
Pengda Liang, Bei Lv, Ke Chen, Dawei L. Sensitive aptasensing of ATP based on a PAM site-regulated CRISPR/Cas12a activation. Mikrochimica acta. vol 191. issue 7. 2024-06-12. PMID:38867016. in the current study, the duplex protospacer adjacent motif (pam) in the activator of crispr/cas12a was used as a molecular switch, and a sensitive adenosine triphosphate (atp) detection biosensor was constructed using an allosteric probe-conjugated pam site formation in hybridization chain reaction (hcr) integrated with the crispr/cas12a system (apf-crispr). 2024-06-12 2024-06-15 Not clear
Jianlin Zheng, Yuwei Zhu, Tengjin Huang, Wenbo Gao, Jiale He, Zhiwei Huan. Inhibition mechanisms of CRISPR-Cas9 by AcrIIA25.1 and AcrIIA32. Science China. Life sciences. 2024-06-06. PMID:38842649. we found that both acriia25.1 and acriia32 directly interact with the wed domain, where they spatially obstruct conformational changes of the wed and pi domains, thereby inhibiting spycas9 from recognizing protospacer adjacent motif (pam) and unwinding double-stranded dna. 2024-06-06 2024-06-08 human
Xu Tang, Ayman Eid, Rui Zhang, Yanhao Cheng, Annan Liu, Yurong Chen, Pengxu Chen, Yong Zhang, Yiping Q. Genome editing in rice and tomato with a small Un1Cas12f1 nuclease. The plant genome. 2024-05-29. PMID:38807445. we characterized the protospacer adjacent motif (pam) requirements and mutation profiles of un1cas12f1 in both plant species. 2024-05-29 2024-06-03 Not clear
Mallesham Bulle, Ajay Kumar Venkatapuram, Sadanandam Abbagani, P B Kirt. CRISPR/Cas9 based genome editing of Phytoene desaturase (PDS) gene in chilli pepper (Capsicum annuum L.). Journal, genetic engineering & biotechnology. vol 22. issue 2. 2024-05-26. PMID:38797550. the detected mutations were mostly 12- to 24-bp deletions that disrupted the exon-intron junction, along with base substitutions and the insertion of 1-bp at the protospacer adjacent motif (pam) region of the target site. 2024-05-26 2024-05-31 Not clear
Xinxin Ke, Ajuan Liang, Chuanxia Chen, Tao H. A one-pot CRISPR-RCA strategy for ultrasensitive and specific detection of circRNA. Analytical methods : advancing methods and applications. vol 16. issue 20. 2024-05-23. PMID:38726809. this method comprises two steps: (1) the rt-rca process of circrna amplification, generating repeat units containing the back-splicing junction (bsj) sequences; and (2) leveraging the protospacer adjacent motif (pam)-independent cas12a/crrna complex to precisely recognize target sequences with bsj, thereby initiating the collateral cleavage activity of cas12a to generate a robust fluorescence signal. 2024-05-23 2024-05-27 Not clear
Yangdao Wei, Yuanzhao Hu, Luchao Wang, Chunsheng Liu, Yuldoshov Sherzod Abdullaewich, Zhiqing Yang, Haimei Mao, Yi Wa. Ultrasensitive detection of Salmonella typhi using a PAM-free Cas14a-based biosensor. Biosensors & bioelectronics. vol 259. 2024-05-23. PMID:38781698. the effectiveness of clustered regularly interspaced short palindromic repeats (crispr)-cas14a1, widely utilized for pathogenic microorganism detection, has been limited by the requirement of a protospacer adjacent motif (pam) on the target dna strands. 2024-05-23 2024-05-27 Not clear
Santosh R Rananaware, Katelyn S Meister, Grace M Shoemaker, Emma K Vesco, Luke Samuel W Sandoval, Jordan G Lewis, August P Bodin, Vedant N Karalkar, Ian H Lange, Brianna Lauren Maria Pizzano, Minji Chang, M Reza Ahmadimashhadi, Sarah J Flannery, Long T Nguyen, Gary P Wang, Piyush K Jai. PAM-free diagnostics with diverse type V CRISPR-Cas systems. medRxiv : the preprint server for health sciences. 2024-05-15. PMID:38746294. however, their dependence on the presence of protospacer adjacent motif (pam) sites on the target double-stranded dna (dsdna) greatly limits their flexibility as diagnostic tools. 2024-05-15 2024-05-27 Not clear
Kyung Eun Lee, Yanping Xu, Bingchuan Geng, Jongsoo Kim, Natalie Kellon, Zhixian He, Zhentao Zhang, Deqiang Li, Doug A Gouchoe, Hua Zh. Prime editing-mediated correction of the leptin receptor in muscle cells of db/db mice. Biotechnology journal. vol 19. issue 5. 2024-05-11. PMID:38730523. additionally, prime editing does not require a precisely positioned protospacer adjacent motif (pam) sequence, which offers flexible target and more precise genomic editing. 2024-05-11 2024-05-27 mouse
Yuedong Zhu, Yanan Lin, Bin Gong, Yan Zhang, Gaoxing Su, Yanyan Y. Dual toeholds regulated CRISPR-Cas12a sensing platform for ApoE single nucleotide polymorphisms genotyping. Biosensors & bioelectronics. vol 255. 2024-04-02. PMID:38565025. different from the conventional single stranded or double stranded activation mode, the dual toeholds regulated mode overcomes protospacer adjacent motif (pam) limitation via cascade toehold mediated strand displacement reaction, which is highly universal and ultra-specific. 2024-04-02 2024-04-05 human
Lei Yang, Guanwei Chen, Jian Wu, Wei Wei, Cheng Peng, Lin Ding, Xiaoyun Chen, Xiaoli Xu, Xiaofu Wang, Junfeng X. A PAM-Free One-Step Asymmetric RPA and CRISPR/Cas12b Combined Assay (OAR-CRISPR) for Rapid and Ultrasensitive DNA Detection. Analytical chemistry. 2024-03-29. PMID:38551977. furthermore, the presence of the protospacer adjacent motif (pam) motif (e.g., ttn or tttn) in the target double-strand dna (dsdna) is an essential prerequisite for the activation of the cas12-based method. 2024-03-29 2024-04-01 Not clear
Dawei Li, Pengda Liang, Shen Ling, Yapeng Wu, Bei L. An optimized microRNA detection platform based on PAM formation-regulated CRISPR/Cas12a activation. International journal of biological macromolecules. 2024-03-23. PMID:38521316. the results showed that different arrangements of the protospacer sequence and protospacer adjacent motif (pam) in the hairpin dna could affect the sensing performance. 2024-03-23 2024-03-26 Not clear
Jiongyu Zhang, Ziyue Li, Chong Guo, Xin Guan, Lori Avery, David Banach, Changchun Li. Intrinsic RNA Targeting Triggers Indiscriminate DNase Activity of CRISPR-Cas12a. Angewandte Chemie (International ed. in English). 2024-03-22. PMID:38516796. we incorporate a t7 transcription step into this assay, thereby eliminating the requirement for a protospacer adjacent motif (pam) sequence in the target. 2024-03-22 2024-03-24 Not clear
Rashid Aman, Muntjeeb M Syed, Ahmed Saleh, Firdaws Melliti, Sivakrishna Rao Gundra, Qiaochu Wang, Tin Marsic, Ahmed Mahas, Magdy M Mahfou. Peptide nucleic acid-assisted generation of targeted double-stranded DNA breaks with T7 endonuclease I. Nucleic acids research. 2024-02-29. PMID:38421613. with no protospacer adjacent motif (pam) constraints and featuring a compact protein size, our pg-t7ei system will facilitate and expand dna manipulations both in vitro and in vivo, including cloning, large-fragment dna assembly, and gene editing, with exciting applications in biotechnology, medicine, agriculture, and synthetic biology. 2024-02-29 2024-03-02 Not clear