| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Ruo-Dan Chen, Yi Yang, Kun-Ming Liu, Jing-Zhen Hu, Yi-Li Feng, Chun-Yi Yang, Rui-Rui Jiang, Si-Cheng Liu, Yue Wang, Ping-An Han, Ru-Gang Tian, Yu-Long Wang, Shi-Ming Xu, An-Yong Xi. Post-cleavage target residence determines asymmetry in non-homologous end joining of Cas12a-induced DNA double strand breaks. Genome biology. vol 26. issue 1. 2025-04-15. PMID:40229905. |
the cas12a-grna complex remains at the protospacer adjacent motif (pam)-proximal end (ppe) while releasing the pam-distal end (pde). |
2025-04-15 |
2025-04-17 |
Not clear |
| Guohui Xiao, Hongyu Shi, Meixia Liu, Min Huang, Siqi Li, Xuefeng Zhou, Hengfei Li, Guoliang Zhan. Trans-cleavage activity of Cas12a effectors can be unleashed by both double-stranded DNA and single-stranded RNA targeting in absence of PAM. International journal of biological macromolecules. 2025-04-13. PMID:40222509. |
crispr-cas12a is a powerful tool in nucleic acid detection, but the relationship between its trans-cleavage activity and protospacer adjacent motif (pam) sequences remains incompletely understood. |
2025-04-13 |
2025-04-16 |
Not clear |
| Haoran Shen, Yanling Li, Kangling Tang, Hongzhi Liang, Zhen-Lin Xu, Yingju Liu, Weipeng Li. Programmable AIESTA: All-in-One Isothermal Enzymatic Signal Transduction Amplifier for Portable Profiling. Analytical chemistry. 2025-03-31. PMID:40162959. |
its potential is further enhanced by its independence from protospacer adjacent motif (pam) requirements and the cost-effectiveness of using short dna guides. |
2025-03-31 |
2025-04-03 |
Not clear |
| Jinlong Ai, Jinhai Deng, Jingjing Hu, Xingxiang Pu, Tongyan Yuan, Yuling Teng, Han Li, Bojie Chen, Jinlian Du, Ling Jiang, Xiaoyan Chen, Erhu Xiong, Ronghua Yan. PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide Variants. JACS Au. vol 5. issue 3. 2025-03-28. PMID:40151256. |
nonetheless, its strict dependency on the presence of a protospacer adjacent motif (pam) within double-stranded dna (dsdna) introduces considerable limitations, thereby constraining its applicability, flexibility, and broader accessibility in molecular diagnostics. |
2025-03-28 |
2025-03-30 |
Not clear |
| Shreya Bhattacharya, Priyadarshi Satpat. Energetics of Expanded PAM Readability by Engineered Cas9-NG. Journal of chemical information and modeling. 2025-03-27. PMID:40146191. |
the energetic basis for the enhanced pam (protospacer adjacent motif) readability in engineered cas9-ng (a variant of cas9 from |
2025-03-27 |
2025-03-30 |
Not clear |
| Min Qing, Cheng Huang, Yueyuan Li, Qiubo Yu, Qianfang Hu, Jing Zhou, Ruo Yuan, Lijuan Ba. Dithiothreitol Facilitates LbCas12a with Expanded PAM Preference for Ultrasensitive Nucleic Acid Detection. Analytical chemistry. 2025-03-15. PMID:40088462. |
however, the requirement of a protospacer adjacent motif (pam) for cas protein-catalyzed trans-cleavage poses a challenge for random nucleic acid detection. |
2025-03-15 |
2025-03-18 |
human |
| Haiyan Shu, Aiping Luan, Hidayat Ullah, Junhu He, You Wang, Chengjie Chen, Qing Wei, Rulin Zhan, Shenghe Chan. Utilizing Target Sequences with Multiple Flanking Protospacer Adjacent Motif (PAM) Sites Reduces Off-Target Effects of the Cas9 Enzyme in Pineapple. Genes. vol 16. issue 2. 2025-02-26. PMID:40004545. |
utilizing target sequences with multiple flanking protospacer adjacent motif (pam) sites reduces off-target effects of the cas9 enzyme in pineapple. |
2025-02-26 |
2025-02-28 |
Not clear |
| Lu Chen, Chen Wang, Zaobing Zhu, Litao Yan. Field-Deployable Detection of Genetically Modified Organisms with an Integrated Method of Loop-Mediated Isothermal Amplification and CRISPR/FnCas12a. Journal of agricultural and food chemistry. 2025-02-18. PMID:39964191. |
by incorporating a universal protospacer adjacent motif (pam) sequence into lamp primers, we overcame the limitations of pam site dependence. |
2025-02-18 |
2025-02-23 |
Not clear |
| Weiwei Zheng, Hongyu Li, Mengxi Liu, Yuhang Wei, Bo Liu, Zekai Li, Chenyang Xiong, Shiqing Huang, Chunyi Hu, Songying Ouyan. Molecular insights and rational engineering of a compact CRISPR-Cas effector Cas12h1 with a broad-spectrum PAM. Signal transduction and targeted therapy. vol 10. issue 1. 2025-02-15. PMID:39955288. |
cas12h1 is a compact crispr-associated nuclease from functionally diverse type v crispr-cas effectors and recognizes a purine-rich protospacer adjacent motif (pam) distinct from that of other type v cas effectors. |
2025-02-15 |
2025-02-18 |
human |
| Kazi A Hossain, Lukasz Nierzwicki, Modesto Orozco, Jacek Czub, Giulia Palerm. Flexibility in PAM recognition expands DNA targeting in xCas9. eLife. vol 13. 2025-02-10. PMID:39928547. |
how xcas9 expands the dna targeting capability of cas9 by recognising a series of alternative protospacer adjacent motif (pam) sequences while ignoring others is unknown. |
2025-02-10 |
2025-02-13 |
Not clear |
| Jin-Ha Song, Seung-Eun Son, Ho-Won Kim, Seung-Ji Kim, Se-Hee An, Chung-Young Lee, Hyuk-Joon Kwon, Kang-Seuk Cho. Rapid and specific on-site H5Nx avian influenza diagnosis via RPA and PAM-independent CRISPR-Cas12a assay combined with anti-NP antibody-based viral RNA purification. Frontiers in veterinary science. vol 12. 2025-02-03. PMID:39896844. |
in this study, we introduce a centrifuge-free, rapid detection method for on-site detection of h5nx viruses that combines magnetic bead-based ribonucleoprotein (rnp) purification and concentration with a crispr-cas12a system that is independent of the protospacer adjacent motif (pam) sequence. |
2025-02-03 |
2025-02-05 |
Not clear |
| Lan Hu, Jing Han, Hao-Da Wang, Zhou-Hua Cheng, Chang-Ce Lv, Dong-Feng Liu, Han-Qing Y. A universal and wide-range cytosine base editor via domain-inlaid and fidelity-optimized CRISPR-FrCas9. Nature communications. vol 16. issue 1. 2025-02-01. PMID:39893181. |
crispr-based base editor (be) offer diverse editing options for genetic engineering of microorganisms, but its application is limited by protospacer adjacent motif (pam) sequences, context preference, editing window, and off-target effects. |
2025-02-01 |
2025-02-04 |
Not clear |
| Xiaojuan Wang, Chenxi Zhao, Nana Yin, Xue Wang, Yang Shu, Jianhua Wan. Dual miRNAs Imaging Platform Based on HRCA-Cas12a by Replacing PAM with Bubble to Reduce False Positive. Analytical chemistry. 2025-01-29. PMID:39876777. |
however, a single target produces significant fluorescent signals leading to false positive due to the presence of protospacer adjacent motif (pam) on the padlock. |
2025-01-29 |
2025-01-31 |
Not clear |
| Wenxue Wang, Shaoya Li, Jiaying Yang, Jingying Li, Lei Yan, Chen Zhang, Yubing He, Lanqin Xi. Exploiting the efficient Exo:Cas12i3-5M fusions for robust single and multiplex gene editing in rice. Journal of integrative plant biology. 2025-01-28. PMID:39873911. |
crispr/cas12i3, which belongs to the class ii type v-i cas system, has attracted extensive attention recently due to its smaller protein size and less restricted canonical "ttn" protospacer adjacent motif (pam). |
2025-01-28 |
2025-01-30 |
Not clear |
| Kazi A Hossain, Lukasz Nierzwicki, Modesto Orozco, Jacek Czub, Giulia Palerm. Flexibility in PAM Recognition Expands DNA Targeting in xCas9. bioRxiv : the preprint server for biology. 2025-01-13. PMID:39803448. |
how xcas9 expands the dna targeting capability of cas9 by recognizing a series of alternative protospacer adjacent motif (pam) sequences while ignoring others is unknown. |
2025-01-13 |
2025-01-16 |
Not clear |
| Zhen Liang, Yuqing Wu, Shuke Deng, Sha Wei, Kai Zhang, Yingjie Gu. Enhanced Genome Editing Activity with Novel Chimeric ScCas9 Variants in Rice. Advanced science (Weinheim, Baden-Wurttemberg, Germany). 2025-01-05. PMID:39755933. |
the streptococcus canis cas9 protein (sccas9) recognizes the nng protospacer adjacent motif (pam), offering a wider range of targets than that offered by the commonly used s. pyogenes cas9 protein (spcas9). |
2025-01-05 |
2025-01-08 |
Not clear |
| Xin Li, Ying Wei, Shu-Yan Wang, Shu-Guang Wang, Peng-Fei Xi. One-for-all gene inactivation via PAM-independent base editing in bacteria. The Journal of biological chemistry. 2024-12-20. PMID:39706269. |
however, the potential of base editing is limited by the adjoined dependence on the editing window and protospacer adjacent motif (pam). |
2024-12-20 |
2024-12-24 |
Not clear |
| Kirsten Bowland, Jiaying Lai, Alyza Skaist, Yan Zhang, Selina Shiqing K Teh, Nicholas J Roberts, Elizabeth Thompson, Sarah J Wheelan, Ralph H Hruban, Rachel Karchin, Matthew H Bailey, Christine A Iacobuzio-Donahue, James R Eshlema. Islands of genomic stability in the face of genetically unstable metastatic cancer. PloS one. vol 19. issue 12. 2024-12-19. PMID:39700179. |
these mutations are targetable because ~10% of them form novel tumor-specific "ngg" protospacer adjacent motif (pam) sites targetable by crispr-cas9. |
2024-12-19 |
2024-12-22 |
Not clear |
| Wenxue Wang, Lei Yan, Jingying Li, Chen Zhang, Yubing He, Shaoya Li, Lanqin Xi. Engineering a robust Cas12i3 variant-mediated wheat genome editing system. Plant biotechnology journal. 2024-12-18. PMID:39690508. |
crispr/cas12i3, which belongs to the type v-i cas system, has attracted extensive attention recently due to its smaller protein size and its less-restricted canonical 'ttn' protospacer adjacent motif (pam). |
2024-12-18 |
2024-12-21 |
Not clear |
| b' Kaitlyn A Kiernan, Jieun Kwon, Bradley J Merrill, Miljan Simonovi\\xc4\\x8. Structural basis of Cas9 DNA interrogation with a 5\\\' truncated sgRNA. Nucleic acids research. 2024-12-10. PMID:39657754.' |
we show that cleavage inhibition can be alleviated by either artificially melting the protospacer adjacent motif (pam)-distal duplex or providing a supercoiled substrate. |
2024-12-10 |
2024-12-13 |
Not clear |