| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Shreya Sirivolu, Chen-Ching Peng, Paolo Neviani, Benjamin Y Xu, Jesse L Berry, Liya X. Comparative Single Vesicle Analysis of Aqueous Humor Extracellular Vesicles before and after Radiation in Uveal Melanoma Eyes. International journal of molecular sciences. vol 25. issue 11. 2024-06-19. PMID:38892225. |
in this study, we used single particle analysis to characterize sev subpopulations in the ah of um patients by quantifying their size, concentration, and phenotypes based on cell surface markers, specifically the tetraspanin co-expression patterns of cd9, cd63, and cd81. |
2024-06-19 |
2024-06-21 |
Not clear |
| Nico Hüttmann, Yingxi Li, Suttinee Poolsup, Emil Zaripov, Rochelle D'Mello, Vanessa Susevski, Zoran Minic, Maxim V Berezovsk. Surface Proteome of Extracellular Vesicles and Correlation Analysis Reveal Breast Cancer Biomarkers. Cancers. vol 16. issue 3. 2024-02-10. PMID:38339272. |
twelve potential biomarker proteins were identified based on cell line-specific expression and filtered by their predicted co-localization with known ev marker proteins, cd63, cd9, and cd81. |
2024-02-10 |
2024-02-12 |
Not clear |
| Koya Yakabi, Eloise Berson, Kathleen S Montine, Sean C Bendall, Michael J MacCoss, Kathleen L Poston, Thomas J Montin. Human cerebrospinal fluid single exosomes in Parkinson's and Alzheimer's diseases. bioRxiv : the preprint server for biology. 2024-01-08. PMID:38187636. |
single events were captured with mouse monoclonal antibodies to one of three different tetraspanins (cd9, cd63, or cd81) or with mouse (m) igg control, and then probed with fluorescently labeled antibodies to prion protein (prp) or cd47 to mark neuronal or presynaptic origin, as well as add- and pdd-related proteins: amyloid beta (aβ), tau, α-synuclein, and apolipoprotein (apo) e. data were collected only from captured events that were within the size range of 50 to 200 nm. |
2024-01-08 |
2024-01-10 |
mouse |
| Dan Yi, Yong-Yi Zhang, Wen-Li Jiang, Mo-Lin Li, Xiang-Hui Chen, Jiang Yu, Hong-Yu Yi, Ya-Qiong Zhu, Yue-Xiang Wan. [Human Platelet-Rich Plasma-Derived Exosomes Promote the Proliferation of Schwann Cells Cultured Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae. 2023-04-28. PMID:37106519. |
methods prp-exos were extracted by polymerization-precipitation combined with ultracentrifugation.the morphology of prp-exos was observed by transmission electron microscopy,and the concentration and particle size distribution of prp-exos were determined by nanoparticle tracking analysis.western blotting was employed to determine the expression of the marker proteins cd63,cd81,and cd9 on exosome surface and the platelet membrane glycoprotein cd41.the scs of rats were isolated and cultured,and the expression of the sc marker s100β was detected by immunofluorescence staining.the fluorescently labeled prp-exos were co-cultured with scs in vitro for observation of their interaction.edu assay was employed to detect the effect of prp-exos on sc proliferation,and cck-8 assay to detect the effects of prp-exos at different concentrations(0,10,20,40,80,and 160 μg/ml)on sc proliferation. |
2023-04-28 |
2023-08-14 |
human |
| b' Jana Jankovi\\xc4\\x8dov\\xc3\\xa1, Petra Se\\xc4\\x8dov\\xc3\\xa1, \\xc4\\xbdubica Horovsk\\xc3\\xa1, Lucia Olexikov\\xc3\\xa1, Linda Duj\\xc3\\xad\\xc4\\x8dkov\\xc3\\xa1, Alexander V Makarevich, Katar\\xc3\\xadna Michalkov\\xc3\\xa1, Jana Antal\\xc3\\xadkov\\xc3\\xa. Distribution of tetraspanins in bovine ovarian tissue and fresh/vitrified oocytes. Histochemistry and cell biology. 2022-10-15. PMID:36242635.' |
the found distribution of tetraspanin cd9, cd63, and integrin alpha v in similar areas of ovarian tissue outlined their possible cooperation. |
2022-10-15 |
2023-08-14 |
cattle |
| Rebecca Lees, Robert Tempest, Alice Law, Dimitri Aubert, Owen G Davies, Soraya Williams, Nick Peake, Ben Peacoc. Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry. Journal of visualized experiments : JoVE. issue 185. 2022-08-15. PMID:35969098. |
here, evs derived from conditioned cell culture media (ccm) are labeled with both a lipid dye, to identify particles with a membrane, and antibodies specific for cd9, cd63, and cd81 as common ev markers. |
2022-08-15 |
2023-08-14 |
Not clear |
| Sara Salvany, Anna Casanovas, Lídia Piedrafita, Olga Tarabal, Sara Hernández, Jordi Calderó, Josep E Esquerd. Microglial recruitment and mechanisms involved in the disruption of afferent synaptic terminals on spinal cord motor neurons after acute peripheral nerve injury. Glia. vol 69. issue 5. 2021-11-16. PMID:33386754. |
the presence of abundant extracellular vesicles in the perineuronal space after axotomy, together with the increased expression of phospho-mixed lineage kinase domain-like protein and, later, of extracellular vesicle markers, such as cd9, cd63, and flotillin, indicate that the vesicles mainly originated in synapses and were transferred to microglia. |
2021-11-16 |
2023-08-13 |
mouse |
| D Yu Zhdanova, R A Poltavtseva, E V Svirshchevskaya, N V Bobkov. Effect of Intranasal Administration of Multipotent Mesenchymal Stromal Cell Exosomes on Memory of Mice in Alzheimer's Disease Model. Bulletin of experimental biology and medicine. vol 170. issue 4. 2021-11-04. PMID:33725248. |
intranasal administration of isolated exosomes expressing typical markers cd9, cd63 and cd81 improved spatial memory in bulbectomized animals, which manifested in a significant increase in the number of visits to the target sector and the time spent there in comparison with indifferent sectors. |
2021-11-04 |
2023-08-13 |
mouse |
| Xiang Cheng, Wen Li, Rongzhen Zhao, Haoming Li, Jianbing Qin, Meiling Tian, Xinhua Zhang, Guohua Ji. The role of hippocampal niche exosomes in rat hippocampal neurogenesis after fimbria-fornix transection. The Journal of biological chemistry. vol 296. 2021-08-30. PMID:33334882. |
additionally, the exosome marker molecules cd9, cd63, and alix were expressed in exosomes extracted from the hippocampal niche. |
2021-08-30 |
2023-08-13 |
rat |
| Niaz Z Khan, Tuoxin Cao, Junyun He, Rodney M Ritzel, Yun Li, Rebecca J Henry, Courtney Colson, Bogdan A Stoica, Alan I Faden, Junfang W. Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential. Brain, behavior, and immunity. vol 92. 2021-05-27. PMID:33307173. |
surface expression of tetraspanins cd9 and cd63 increased in multiple cell types at the injury site; however, astrocyte cd81 expression uniquely decreased, as demonstrated by flow cytometry. |
2021-05-27 |
2023-08-13 |
mouse |
| Ke Hu, Xianhao Liu, Yi Li, Qinyang Li, Yijun Xu, Wei Zeng, Guocheng Zhong, Changhua Y. Exosomes Mediated Transfer of Circ_UBE2D2 Enhances the Resistance of Breast Cancer to Tamoxifen by Binding to MiR-200a-3p. Medical science monitor : international medical journal of experimental and clinical research. vol 26. 2021-05-20. PMID:32756532. |
western blot was used to detect the levels of estrogen receptor alpha (eralpha), e-cadherin, vimentin, cd9, and cd63. |
2021-05-20 |
2023-08-13 |
Not clear |
| Keerthie Dissanayake, Monika Nõmm, Freddy Lättekivi, Yosra Ressaissi, Kasun Godakumara, Arina Lavrits, Getnet Midekessa, Janeli Viil, Rikke Bæk, Malene Møller Jørgensen, Sourav Bhattacharjee, Aneta Andronowska, Andres Salumets, Ülle Jaakma, Alireza Fazel. Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers. Theriogenology. vol 149. 2021-01-21. PMID:32259747. |
based on ev array, evs isolated from embryo conditioned media were strongly positive for ev-markers cd9 and cd81 and weakly positive for cd63 and alix among others. |
2021-01-21 |
2023-08-13 |
cattle |
| Ye Tian, Manfei Gong, Yunyun Hu, Haisheng Liu, Wenqiang Zhang, Miaomiao Zhang, Xiuxiu Hu, Dimitri Aubert, Shaobin Zhu, Lina Wu, Xiaomei Ya. Quality and efficiency assessment of six extracellular vesicle isolation methods by nano-flow cytometry. Journal of extracellular vesicles. vol 9. issue 1. 2020-10-24. PMID:31839906. |
as cd9, cd63 and cd81 also exist in the plasma matrix, single-particle phenotyping of evs offers distinct advantage in the validation of evs compared with ensemble-averaged approaches, such as western blot analysis. |
2020-10-24 |
2023-08-13 |
Not clear |
| Lisa Seipold, Paul Safti. The Emerging Role of Tetraspanins in the Proteolytic Processing of the Amyloid Precursor Protein. Frontiers in molecular neuroscience. vol 9. 2020-10-01. PMID:28066176. |
cd9, cd63, cd81, tspan12, tspan15 are among the tetraspanins involved in the intracellular transport and in the stabilization of the gamma secretase complex or adam10 as the major app alpha secretase. |
2020-10-01 |
2023-08-13 |
Not clear |
| Li-Mei Zhang, Li-Li Jia, Wen-Li Y. [Serum exosomes of rats with hepatic ischemia-reperfusion injury induce the damage of hippocampus and cerebral cortex]. Sheng li xue bao : [Acta physiologica Sinica]. vol 72. issue 4. 2020-09-24. PMID:32820307. |
the morphology and size of exosomes were observed by transmission electron microscope, the expression of exosomes markers cd63 and cd9 was detected by western blot, and the damage of liver and brain, levels of apoptosis and oxidative stress response in hippocampus and cerebral cortex were observed by serological and histological indexes. |
2020-09-24 |
2023-08-13 |
rat |
| Wei Seong Toh, Ruenn Chai Lai, Bin Zhang, Sai Kiang Li. MSC exosome works through a protein-based mechanism of action. Biochemical Society transactions. vol 46. issue 4. 2019-02-18. PMID:29986939. |
nevertheless, the term 'msc exosome' is often used to describe populations of 50-200 nm evs that are prepared from culture medium conditioned by mscs on the basis that these populations collectively exhibited typical exosome-associated proteins such as endosomal proteins, tsg101 and alix, and tetraspanin proteins, cd9, cd63 and cd81. |
2019-02-18 |
2023-08-13 |
Not clear |
| b' Elisa Giacomini, Riccardo Vago, Ana Maria Sanchez, Paola Podini, Natasa Zarovni, Valentina Murdica, Roberta Rizzo, Daria Bortolotti, Massimo Candiani, Paola Vigan\\xc3\\xb. Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side. Scientific reports. vol 7. issue 1. 2019-02-06. PMID:28701751.' |
here we demonstrate for the first time that conditioned media from non-manipulated human embryos cultured in vitro for 3 days or up to the blastocyst stage contain extracellular vesicles (evs) with a diameter of 50 to 200 nm and bearing the traditional microvesicle and exosome marker proteins cd63, cd9 and alix. |
2019-02-06 |
2023-08-13 |
human |
| Fan-Long Kong, Xiao-Ping Wang, Ya-Nan Li, Hai-Xu Wan. The role of exosomes derived from cerebrospinal fluid of spinal cord injury in neuron proliferation in vitro. Artificial cells, nanomedicine, and biotechnology. vol 46. issue 1. 2018-08-01. PMID:28346015. |
the results demonstrated these exosomes all expressed cd9, cd63, cd81, alix and tsg101; however, only exosomes derived from cerebrospinal fluid of sci could promote proliferation of neuron via erk signaling pathway, and decrease cell apoptosis. |
2018-08-01 |
2023-08-13 |
Not clear |
| Rosario Castro, Beatriz Abós, Lucia González, Carolina Aquilino, Jaime Pignatelli, Carolina Tafall. Molecular characterization of CD9 and CD63, two tetraspanin family members expressed in trout B lymphocytes. Developmental and comparative immunology. vol 51. issue 1. 2016-02-17. PMID:25769915. |
throughout the early life cycle stages, cd9 mrna levels significantly increased after first feeding, whereas cd63 transcription remained constant during all the developmental stages analyzed. |
2016-02-17 |
2023-08-13 |
Not clear |
| Louise A Koopman, Hernan D Kopcow, Basya Rybalov, Jonathan E Boyson, Jordan S Orange, Frederick Schatz, Rachel Masch, Charles J Lockwood, Asher D Schachter, Peter J Park, Jack L Strominge. Human decidual natural killer cells are a unique NK cell subset with immunomodulatory potential. The Journal of experimental medicine. vol 198. issue 8. 2003-11-24. PMID:14568979. |
the largest number of these encoded surface proteins, including the unusual lectinlike receptors nkg2e and ly-49l, several killer cell ig-like receptors, the integrin subunits alpha(d), alpha(x), beta1, and beta5, and multiple tetraspanins (cd9, cd151, cd53, cd63, and tspan-5). |
2003-11-24 |
2023-08-12 |
human |