| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Shreya Sirivolu, Chen-Ching Peng, Paolo Neviani, Benjamin Y Xu, Jesse L Berry, Liya X. Comparative Single Vesicle Analysis of Aqueous Humor Extracellular Vesicles before and after Radiation in Uveal Melanoma Eyes. International journal of molecular sciences. vol 25. issue 11. 2024-06-19. PMID:38892225. |
in this study, we used single particle analysis to characterize sev subpopulations in the ah of um patients by quantifying their size, concentration, and phenotypes based on cell surface markers, specifically the tetraspanin co-expression patterns of cd9, cd63, and cd81. |
2024-06-19 |
2024-06-21 |
Not clear |
| Nico Hüttmann, Yingxi Li, Suttinee Poolsup, Emil Zaripov, Rochelle D'Mello, Vanessa Susevski, Zoran Minic, Maxim V Berezovsk. Surface Proteome of Extracellular Vesicles and Correlation Analysis Reveal Breast Cancer Biomarkers. Cancers. vol 16. issue 3. 2024-02-10. PMID:38339272. |
twelve potential biomarker proteins were identified based on cell line-specific expression and filtered by their predicted co-localization with known ev marker proteins, cd63, cd9, and cd81. |
2024-02-10 |
2024-02-12 |
Not clear |
| Koya Yakabi, Eloise Berson, Kathleen S Montine, Sean C Bendall, Michael J MacCoss, Kathleen L Poston, Thomas J Montin. Human cerebrospinal fluid single exosomes in Parkinson's and Alzheimer's diseases. bioRxiv : the preprint server for biology. 2024-01-08. PMID:38187636. |
single events were captured with mouse monoclonal antibodies to one of three different tetraspanins (cd9, cd63, or cd81) or with mouse (m) igg control, and then probed with fluorescently labeled antibodies to prion protein (prp) or cd47 to mark neuronal or presynaptic origin, as well as add- and pdd-related proteins: amyloid beta (aβ), tau, α-synuclein, and apolipoprotein (apo) e. data were collected only from captured events that were within the size range of 50 to 200 nm. |
2024-01-08 |
2024-01-10 |
mouse |
| Dan Yi, Yong-Yi Zhang, Wen-Li Jiang, Mo-Lin Li, Xiang-Hui Chen, Jiang Yu, Hong-Yu Yi, Ya-Qiong Zhu, Yue-Xiang Wan. [Human Platelet-Rich Plasma-Derived Exosomes Promote the Proliferation of Schwann Cells Cultured Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae. 2023-04-28. PMID:37106519. |
methods prp-exos were extracted by polymerization-precipitation combined with ultracentrifugation.the morphology of prp-exos was observed by transmission electron microscopy,and the concentration and particle size distribution of prp-exos were determined by nanoparticle tracking analysis.western blotting was employed to determine the expression of the marker proteins cd63,cd81,and cd9 on exosome surface and the platelet membrane glycoprotein cd41.the scs of rats were isolated and cultured,and the expression of the sc marker s100β was detected by immunofluorescence staining.the fluorescently labeled prp-exos were co-cultured with scs in vitro for observation of their interaction.edu assay was employed to detect the effect of prp-exos on sc proliferation,and cck-8 assay to detect the effects of prp-exos at different concentrations(0,10,20,40,80,and 160 μg/ml)on sc proliferation. |
2023-04-28 |
2023-08-14 |
human |
| Rebecca Lees, Robert Tempest, Alice Law, Dimitri Aubert, Owen G Davies, Soraya Williams, Nick Peake, Ben Peacoc. Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry. Journal of visualized experiments : JoVE. issue 185. 2022-08-15. PMID:35969098. |
here, evs derived from conditioned cell culture media (ccm) are labeled with both a lipid dye, to identify particles with a membrane, and antibodies specific for cd9, cd63, and cd81 as common ev markers. |
2022-08-15 |
2023-08-14 |
Not clear |
| D Yu Zhdanova, R A Poltavtseva, E V Svirshchevskaya, N V Bobkov. Effect of Intranasal Administration of Multipotent Mesenchymal Stromal Cell Exosomes on Memory of Mice in Alzheimer's Disease Model. Bulletin of experimental biology and medicine. vol 170. issue 4. 2021-11-04. PMID:33725248. |
intranasal administration of isolated exosomes expressing typical markers cd9, cd63 and cd81 improved spatial memory in bulbectomized animals, which manifested in a significant increase in the number of visits to the target sector and the time spent there in comparison with indifferent sectors. |
2021-11-04 |
2023-08-13 |
mouse |
| Tanina Arab, Emily R Mallick, Yiyao Huang, Liang Dong, Zhaohao Liao, Zezhou Zhao, Olesia Gololobova, Barbara Smith, Norman J Haughey, Kenneth J Pienta, Barbara S Slusher, Patrick M Tarwater, Juan Pablo Tosar, Angela M Zivkovic, Wyatt N Vreeland, Michael E Paulaitis, Kenneth W Witwe. Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single-particle analysis platforms. Journal of extracellular vesicles. vol 10. issue 6. 2021-09-15. PMID:33850608. |
evs from the human t lymphocyte line h9 (high cd81, low cd63) and the promonocytic line u937 (low cd81, high cd63) were separated from culture conditioned medium (ccm) by differential ultracentrifugation (duc) or a combination of ultrafiltration (uf) and size exclusion chromatography (sec) and characterized by transmission electron microscopy (tem) and western blot (wb). |
2021-09-15 |
2023-08-13 |
human |
| Niaz Z Khan, Tuoxin Cao, Junyun He, Rodney M Ritzel, Yun Li, Rebecca J Henry, Courtney Colson, Bogdan A Stoica, Alan I Faden, Junfang W. Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential. Brain, behavior, and immunity. vol 92. 2021-05-27. PMID:33307173. |
surface expression of tetraspanins cd9 and cd63 increased in multiple cell types at the injury site; however, astrocyte cd81 expression uniquely decreased, as demonstrated by flow cytometry. |
2021-05-27 |
2023-08-13 |
mouse |
| Keerthie Dissanayake, Monika Nõmm, Freddy Lättekivi, Yosra Ressaissi, Kasun Godakumara, Arina Lavrits, Getnet Midekessa, Janeli Viil, Rikke Bæk, Malene Møller Jørgensen, Sourav Bhattacharjee, Aneta Andronowska, Andres Salumets, Ülle Jaakma, Alireza Fazel. Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers. Theriogenology. vol 149. 2021-01-21. PMID:32259747. |
based on ev array, evs isolated from embryo conditioned media were strongly positive for ev-markers cd9 and cd81 and weakly positive for cd63 and alix among others. |
2021-01-21 |
2023-08-13 |
cattle |
| Ye Tian, Manfei Gong, Yunyun Hu, Haisheng Liu, Wenqiang Zhang, Miaomiao Zhang, Xiuxiu Hu, Dimitri Aubert, Shaobin Zhu, Lina Wu, Xiaomei Ya. Quality and efficiency assessment of six extracellular vesicle isolation methods by nano-flow cytometry. Journal of extracellular vesicles. vol 9. issue 1. 2020-10-24. PMID:31839906. |
as cd9, cd63 and cd81 also exist in the plasma matrix, single-particle phenotyping of evs offers distinct advantage in the validation of evs compared with ensemble-averaged approaches, such as western blot analysis. |
2020-10-24 |
2023-08-13 |
Not clear |
| Lisa Seipold, Paul Safti. The Emerging Role of Tetraspanins in the Proteolytic Processing of the Amyloid Precursor Protein. Frontiers in molecular neuroscience. vol 9. 2020-10-01. PMID:28066176. |
cd9, cd63, cd81, tspan12, tspan15 are among the tetraspanins involved in the intracellular transport and in the stabilization of the gamma secretase complex or adam10 as the major app alpha secretase. |
2020-10-01 |
2023-08-13 |
Not clear |
| Wei Seong Toh, Ruenn Chai Lai, Bin Zhang, Sai Kiang Li. MSC exosome works through a protein-based mechanism of action. Biochemical Society transactions. vol 46. issue 4. 2019-02-18. PMID:29986939. |
nevertheless, the term 'msc exosome' is often used to describe populations of 50-200 nm evs that are prepared from culture medium conditioned by mscs on the basis that these populations collectively exhibited typical exosome-associated proteins such as endosomal proteins, tsg101 and alix, and tetraspanin proteins, cd9, cd63 and cd81. |
2019-02-18 |
2023-08-13 |
Not clear |
| Fan-Long Kong, Xiao-Ping Wang, Ya-Nan Li, Hai-Xu Wan. The role of exosomes derived from cerebrospinal fluid of spinal cord injury in neuron proliferation in vitro. Artificial cells, nanomedicine, and biotechnology. vol 46. issue 1. 2018-08-01. PMID:28346015. |
the results demonstrated these exosomes all expressed cd9, cd63, cd81, alix and tsg101; however, only exosomes derived from cerebrospinal fluid of sci could promote proliferation of neuron via erk signaling pathway, and decrease cell apoptosis. |
2018-08-01 |
2023-08-13 |
Not clear |
| Michael A Chernousov, Richard C Stahl, David J Care. Tetraspanins are involved in Schwann cell-axon interaction. Journal of neuroscience research. vol 91. issue 11. 2014-05-02. PMID:24038174. |
suppression of cd63 and cd81 expression did not affect the ability of schwann cells to myelinate dorsal root ganglion neurons in vitro. |
2014-05-02 |
2023-08-12 |
Not clear |
| Nanette Van Damme, John Guatell. HIV-1 Vpu inhibits accumulation of the envelope glycoprotein within clathrin-coated, Gag-containing endosomes. Cellular microbiology. vol 10. issue 5. 2008-05-02. PMID:18076669. |
these structures had several features consistent with an endosomal viral assembly domain: they contained gag, including proteolytically processed viral matrix protein; the tetraspanins cd63 and cd81; the adaptor protein complex ap-3; and aip1/alix, a cellular cofactor for viral budding. |
2008-05-02 |
2023-08-12 |
Not clear |
| Fedor Berditchevski, Elena Odintsova, Shigeaki Sawada, Elizabeth Gilber. Expression of the palmitoylation-deficient CD151 weakens the association of alpha 3 beta 1 integrin with the tetraspanin-enriched microdomains and affects integrin-dependent signaling. The Journal of biological chemistry. vol 277. issue 40. 2002-11-20. PMID:12110679. |
the association of the palmitoylation-deficient mutant of cd151 (cd151cys8) with cd81 and cd63 was markedly decreased, but the interaction of the alpha(3)beta(1)-cd151cys8 complex with phosphatidylinositol 4-kinase was not affected. |
2002-11-20 |
2023-08-12 |
Not clear |
| Fedor Berditchevski, Elena Odintsova, Shigeaki Sawada, Elizabeth Gilber. Expression of the palmitoylation-deficient CD151 weakens the association of alpha 3 beta 1 integrin with the tetraspanin-enriched microdomains and affects integrin-dependent signaling. The Journal of biological chemistry. vol 277. issue 40. 2002-11-20. PMID:12110679. |
ectopic expression of cd151cys8 in rat-1 cells impaired the interactions of the endogenous cd63 and cd81 with the alpha(3)beta(1) integrin. |
2002-11-20 |
2023-08-12 |
Not clear |
| C Claas, C S Stipp, M E Hemle. Evaluation of prototype transmembrane 4 superfamily protein complexes and their relation to lipid rafts. The Journal of biological chemistry. vol 276. issue 11. 2001-06-21. PMID:11113129. |
here we show that prototype complexes of cd9 or cd81 (tm4sf proteins) with alpha(3)beta(1) (an integrin) and complexes of cd63 (a tm4sf protein) with phosphatidylinositol 4-kinase (ptdins 4-k) may indeed localize within lipid raft-like microdomains, as seen by three different criteria. |
2001-06-21 |
2023-08-12 |
Not clear |
| H Okochi, M Kato, K Nashiro, O Yoshie, K Miyazono, M Furu. Expression of tetra-spans transmembrane family (CD9, CD37, CD53, CD63, CD81 and CD82) in normal and neoplastic human keratinocytes: an association of CD9 with alpha 3 beta 1 integrin. The British journal of dermatology. vol 137. issue 6. 1998-02-23. PMID:9470900. |
tetra-spans transmembrane family (tstf) members (cd9, cd37, cd53, cd63, cd81 and cd82) have potent effects on cell growth, motility and adhesion in various cells. |
1998-02-23 |
2023-08-12 |
human |
| H Okochi, M Kato, K Nashiro, O Yoshie, K Miyazono, M Furu. Expression of tetra-spans transmembrane family (CD9, CD37, CD53, CD63, CD81 and CD82) in normal and neoplastic human keratinocytes: an association of CD9 with alpha 3 beta 1 integrin. The British journal of dermatology. vol 137. issue 6. 1998-02-23. PMID:9470900. |
expression of tetra-spans transmembrane family (cd9, cd37, cd53, cd63, cd81 and cd82) in normal and neoplastic human keratinocytes: an association of cd9 with alpha 3 beta 1 integrin. |
1998-02-23 |
2023-08-12 |
human |