All Relations between blot and neurotransmitter

Publication Sentence Publish Date Extraction Date Species
Yanlin Tao, Wei Shen, Houyuan Zhou, Zikang Li, Ting Pi, Hui Wu, Hailian Shi, Fei Huang, Xiaojun W. Sex differences in a corticosterone-induced depression model in mice: Behavioral, neurochemical, and molecular insights. Brain research. 2023-11-18. PMID:37979605. behavioral tests, nissl staining, uplc-ms/ms, and western blot analysis were performed to assess behavioral changes, as well as neuronal alterations, neurotransmitter levels, and protein expressions in the hippocampus. 2023-11-18 2023-11-20 mouse
Jian-Bo Chen, Mei-Jia Li, Xiao-Hui Huo, Tie-Jun Gong, Xiao Han, Jin-Hui Liu, Shuang Liu, Yin-Shi Su. Ginsenoside Rg5 Improves Sleep by Regulating Energy Metabolism in Sleep-Deprived Rats. The American journal of Chinese medicine. 2023-09-05. PMID:37667863. a western blot analysis and elisa were used to detect the metabolic levels, mitochondrial functional proteins, ampk pathway proteins, clock-related proteins, adenosine receptors, and neurotransmitter receptors. 2023-09-05 2023-09-07 rat
Qi Ma, Li Wang, Xiang-Xin Liu, Zhi-Guo An, Xiao Luo, Li-Li Zhang, Ping Yan, Lu Jin, Ren Cai, Qi-Zhong Y. GLP-1 plays a protective role in hippocampal neuronal cells by activating cAMP-CREB-BDNF signaling pathway against CORT+HG-induced toxicity. Heliyon. vol 9. issue 8. 2023-08-14. PMID:37576283. in addition, bdnf and neurotransmitter levels, lactic dehydrogenase (ldh) and glucose levels, and proteins of camp-creb-bdnf signal pathway in the culture supernatants were measured through an enzyme-linked immunosorbent assay and colorimetric assays and western blot, respectively. 2023-08-14 2023-08-16 mouse
Yihuai Peng, Zhen Du, Jianxiu Duan, Yaqin Shao, Shengdan Ni. MiR-107 Activates NF- κ B versus A β Analysis of the regulatory effect of 1-42 induced apoptosis in Alzheimer's disease cells. Cellular and molecular biology (Noisy-le-Grand, France). vol 69. issue 3. 2023-06-11. PMID:37300691. experiments conducted using the dual luciferase method and western blot analysis also showed that mir-107 in primary neurons affects neurotransmitter factors in alzheimer's disease through the regulation of the nf-κb signaling pathway. 2023-06-11 2023-08-14 Not clear
Feng Wang, Hongwei Wu, Azhen Hu, Lei Dong, Xiaoxia Lin, Menghao Li, Yongling Wang, Wenjun Li, Liansheng Chang, Yuqiao Chang, Hanqing Liu, Yu Shi, Nana L. Ultrasound combined with glial cell line-derived neurotrophic factor-loaded microbubbles for the targeted treatment of drug addiction. Frontiers in bioengineering and biotechnology. vol 10. 2022-09-01. PMID:36046678. western blot was used to detect changes in tyrosine hydroxylase (th) expression levels in the vta region after different treatments, and high performance liquid chromatography was used to detect the changes in monoamine neurotransmitter content. 2022-09-01 2023-08-14 rat
Ayane Ninomiya, Abdallah Mshaty, Asahi Haijima, Hiroyuki Yajima, Michifumi Kokubo, Miski Aghnia Khairinisa, Winda Ariyani, Yuki Fujiwara, Sumiyasu Ishii, Nobutake Hosoi, Hirokazu Hirai, Izuki Amano, Noriyuki Koibuch. The neurotoxic effect of lactational PFOS exposure on cerebellar functional development in male mice. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2021-12-06. PMID:34871666. western blot analysis indicated that pfos exposure increased syntaxin binding protein 1 (munc18-1) and glutamate metabotropic receptor 1 (mglur1) protein levels, which may be associated with the change in neurotransmitter release from parallel fibers and the level of long-term depression, respectively. 2021-12-06 2023-08-13 mouse
Jian-Ping Zhu, Hua-Ying Wu, Yuan Zi, Xin-Bin Xia, Meng-Zhou Xie, Zhi-Ying Yua. Baihe Jizihuang Tang Ameliorates Chronic Unpredictable Mild Stress-Induced Depression-Like Behavior: Integrating Network Pharmacology and Brain-Gut Axis Evaluation. Evidence-based complementary and alternative medicine : eCAM. vol 2021. 2021-09-04. PMID:34475963. network pharmacological analysis; chronic unpredictable mild stress (cums) rat model assessment; behavioral tests; analysis of hippocampal neurotransmitter levels, hippocampal pathological structure, and hypothalamic-pituitary-adrenal (hpa) axis; western blot analysis; 16s rna sequencing; ultraperformance liquid chromatography (uplc)/mass spectrometry (ms); and high-performance liquid chromatography (hplc)/ultraviolet (uv) analysis were used. 2021-09-04 2023-08-13 rat
He Yu, Zhong-Mei He, Kun Shi, Yan Zhao, Ying Zong, Jian-Ming Li, Wei-Jia Chen, Rui D. [Study on antidepressant effect of total alkaloids of Fibraurea recisa]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 46. issue 14. 2021-08-18. PMID:34402292. this study aimed to investigate the antidepressant effects of total alkaloids of fibraurea recisa in ht22 cells damaged by corticosterone (cort) in vitro and in a mouse model of chronic unpredictable mild stress (cums) as well as the underlying cellular experiments,the viability of cort-damaged ht22 cells was detected using cell counting kit-8 (cck-8),and the cell apoptosis was detected by hoechst 33258 animal experiments,c57bl/6n mice were randomly divided into the control group,model group,low (100 mg·kg~(-1)),medium (200 mg·kg~(-1)) and high (400 mg·kg~(-1))-dose of total alkaloids of f.recisa groups,and positive control group.after 21 days of cums exposure,their depressive behaviors were observed in behavioral and morris water maze tests.the serum levels of 5-hydroxytryptamine (5-ht),dopamine (da),and norepinephrine (ne) were assessed by elisa.the expression levels of apoptosis-related proteins bcl-2,bax and cleaved caspase-3 in ht22 cells and mouse hippocampus were detected by western blot.the results suggested that total alkaloids of f.recisa alleviated the damage of ht22 cells induced by cort in a dose-dependent manner.the hoechst 33258 staining uncovered that total alkaloids of f.recisa better reduced the blue spots and inhibited cell apoptosis.the results of animal experiments showed that total alkaloids of f.recisa significantly improved the depression-like behaviors of mice and increased the serum levels of 5-ht,da and ne as compared with those in the model group.the western blot assays revealed a significant up-regulation of bcl-2 protein expression,but an obvious reduction in bax and cleaved caspase-3protein expression in the total alkaloids of f.recisa conclusion,total alkaloids of f.recisa inhibited depression possibly by regulating the apoptosis-related protein expression or elevating the monoamine neurotransmitter levels in the brain. 2021-08-18 2023-08-13 mouse
Xu-Dong Ding, Wei Wang, Zhi-Gang Ding, Yan-Ping Liu, Jing Zhong, Hua-Xian Che. Impact of Botox-A SNAP-25 protein expression and the mechanism of inhibitory neurotransmitter imbalance in chronic sciatic nerve pain rat model. Experimental and therapeutic medicine. vol 13. issue 6. 2020-09-29. PMID:28588664. snap-25 protein expression level, mrna subunit nr2b within excitatory neurotransmitter glutamate glt and protein expression level, as well as gat mrna, the inhibitory gaba neurotransmitter transporter and protein expression level were studied by rt-polymerase chain reaction and western blot analysis. 2020-09-29 2023-08-13 rat
Chenyang Han, Li Guo, Yi Yang, WenYan Li, YongJia Sheng, Jin Wang, Qiaobing Guan, Xiaoling Zhan. Study on antrodia camphorata polysaccharide in alleviating the neuroethology of PD mice by decreasing the expression of NLRP3 inflammasome. Phytotherapy research : PTR. vol 33. issue 9. 2019-11-19. PMID:31359520. after antrodia camphorata polysaccharide (acp) intervention, neurobehavioral changes were detected, neurotransmitter changes in striatum were determined by high-performance liquid chromatography, the alterations of striatal nod-like receptor pyrin domain containing three (nlrp3) were examined by immunohistochemistry, and the expression of nlrp3, il-1β, caspase-1, and procaspase-1 were detected by western blot. 2019-11-19 2023-08-13 mouse
Catherine Lévesque, Giovanni Hernandez, Souha Mahmoudi, Frédéric Calon, Fabrizio Gasparini, Baltazar Gomez-Mancilla, Pierre J Blanchet, Daniel Lévesqu. Deficient striatal adaptation in aminergic and glutamatergic neurotransmission is associated with tardive dyskinesia in non-human primates exposed to antipsychotic drugs. Neuroscience. vol 361. 2018-06-14. PMID:28790021. using autoradiography, western blot and in situ hybridization techniques, we compared neurochemical components of the dopamine, serotonin, and glutamate neurotransmitter systems modulating striatal activity in monkeys chronically exposed to haloperidol and clozapine. 2018-06-14 2023-08-13 human
Jinfang Yang, Qian Wang, Fenfen He, Yanxia Ding, Qingyan Sun, Tianmiao Hua, Minmin X. Dietary Restriction Affects Neuronal Response Property and GABA Synthesis in the Primary Visual Cortex. PloS one. vol 11. issue 2. 2016-07-25. PMID:26863207. to clarify this issue, we examined the response properties of neurons in the primary visual cortex (v1) of dr and control groups of cats using in vivo extracellular single-unit recording techniques, and assessed the synthesis of inhibitory neurotransmitter gaba in the v1 of cats from both groups using immunohistochemical and western blot techniques. 2016-07-25 2023-08-13 Not clear
Evert-Jan Kooi, Marloes Prins, Natasha Bajic, Jeroen A M Beliën, Wouter H Gerritsen, Jack van Horssen, Eleonora Aronica, Anne-Marie van Dam, Jeroen J M Hoozemans, Paul T Francis, Paul van der Valk, Jeroen J G Geurt. Cholinergic imbalance in the multiple sclerosis hippocampus. Acta neuropathologica. vol 122. issue 3. 2012-01-10. PMID:21691765. by means of biochemical assessment, (immuno)histochemistry and western blot analyses, we detected substantial alterations in the cholinergic neurotransmitter system in the ms hippocampus, which were different from those in ad hippocampus. 2012-01-10 2023-08-12 Not clear
Angela S Pechenino, Karyn M Fric. The effects of acute 17beta-estradiol treatment on gene expression in the young female mouse hippocampus. Neurobiology of learning and memory. vol 91. issue 3. 2009-04-27. PMID:18938255. mrna expression changes in 5 of the genes were confirmed by real-time quantitative pcr analysis, and protein changes in these same genes were confirmed by western blot analysis: hsp70, a heat shock protein known to be estrogen responsive; igfbp2, an igf-i binding protein; actn4, an actin binding protein involved in protein trafficking; tubb2a, the major component of microtubules; and snap25, a synaptosome-specific protein required for neurotransmitter release. 2009-04-27 2023-08-12 mouse
K E Borgmann-Winter, N E Rawson, H-Y Wang, H Wang, M L Macdonald, M H Ozdener, K K Yee, G Gomez, J Xu, B Bryant, G Adamek, N Mirza, E Pribitkin, C-G Hah. Human olfactory epithelial cells generated in vitro express diverse neuronal characteristics. Neuroscience. vol 158. issue 2. 2009-04-15. PMID:18996445. western blot analysis revealed the expression of neurotransmitter receptors for dopamine (d2r), 5-ht (5ht2c) and nmda subtypes 1 and 2a/2b. 2009-04-15 2023-08-12 human
Marcé D Lorenzen, Andreas Gnirke, Jonathan Margolis, Jeffrey Garnes, Margie Campbell, Jeffrey J Stuart, Rajat Aggarwal, Stephen Richards, Yoonseong Park, Richard W Beema. The maternal-effect, selfish genetic element Medea is associated with a composite Tc1 transposon. Proceedings of the National Academy of Sciences of the United States of America. vol 105. issue 29. 2008-08-19. PMID:18621706. we show that medea(1) activity in tribolium castaneum is associated with a composite tc1 transposon inserted just downstream of the neurotransmitter reuptake symporter bloated tubules (blot), whose drosophila ortholog has both maternal and zygotic functions. 2008-08-19 2023-08-12 drosophila_melanogaster
Regina Celia Cussa Kubrusly, Maria Cristina Caldas da Cunha, Ricardo Augusto de Melo Reis, Heline Soares, Ana Lúcia Marques Ventura, Eleonora Kurtenbach, Maria Cristina Fialho de Mello, Fernando Garcia de Mell. Expression of functional receptors and transmitter enzymes in cultured Muller cells. Brain research. vol 1038. issue 2. 2005-05-24. PMID:15757630. we developed a purified muller glia culture to investigate the expression of several neurotransmitter markers on these cells, such as dopaminergic, cholinergic, gabaergic and peptidergic receptors or enzymes, based on functional assays measuring second messenger levels or western blot for specific proteins. 2005-05-24 2023-08-12 chicken
K Johnson, E Knust, H Skae. bloated tubules (blot) encodes a Drosophila member of the neurotransmitter transporter family required for organisation of the apical cytocortex. Developmental biology. vol 212. issue 2. 1999-09-09. PMID:10433833. bloated tubules (blot) encodes a drosophila member of the neurotransmitter transporter family required for organisation of the apical cytocortex. 1999-09-09 2023-08-12 drosophila_melanogaster
G Barbany, W J Friedman, H Persso. Lymphocyte-mediated regulation of neurotransmitter gene expression in rat sympathetic ganglia. Journal of neuroimmunology. vol 32. issue 2. 1991-05-13. PMID:1672871. northern blot analysis was used to measure changes in neurotransmitter mrna expression. 1991-05-13 2023-08-11 rat