| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Ziyang Ye, Zhihan Ge, Shu Yang, Ting Hu, Qiong Ye, Hui Che. Scutellarein alleviates osteoarthritis progression through the PI3K/Akt/NF-kappaB signaling pathway: In vitro and in vivo studies. Phytotherapy research : PTR. 2024-05-02. PMID:38695125. |
western blot, qpcr, as well as elisa were utilized to measure extracellular matrix (ecm) degradation and inflammation. |
2024-05-02 |
2024-05-04 |
Not clear |
| P G Roopashree, Shilpa S Shetty, Vijith Vittal Shetty, P C Suhasini, Kumari N Sucheth. Inhibitory effects of medium-chain fatty acids on the proliferation of human breast cancer cells via suppression of Akt/mTOR pathway and modulating the Bcl-2 family protein. Journal of cellular biochemistry. 2024-04-26. PMID:38666486. |
to investigate the apoptosis-inducing effect of mcfas in breast cancer cells, cell viability assay, colony formation assay, cell migration assay, cell invasion assay, nuclear morphology, cell cycle assay, intracellular reactive oxygen species (ros), matrix metalloproteinase (mmp), apoptosis, rt-qpcr analysis, and western blot analysis were performed. |
2024-04-26 |
2024-04-28 |
human |
| Mohammad S El-Wetidy, Mohamad I Rady, Islam Rady, Hamed Hela. Urolithin A affects cellular migration and modulates matrix metalloproteinase expression in colorectal cancer cells. Cell biochemistry and function. vol 42. issue 3. 2024-04-16. PMID:38622949. |
the western blot analysis investigation revealed that ua inhibits cellular metastasis by lowering the expression levels of matrix metalloproteinases 1 and 2 (mmp1 and mmp2) by more than 43% and 41% (p < .001) in ht29, 28% and 149% (p < .001) in sw480, and 90% and 74% (p < .001) in sw620, respectively, at a 100 µm dosage of ua compared to the control. |
2024-04-16 |
2024-04-18 |
Not clear |
| Sha Wu, Haiqi Zhou, Huixian Ling, Yuyan Sun, Ziyu Luo, ThaiNamanh Ngo, Yuanyuan Fu, Wen Wang, Ying Kon. LIPUS regulates the progression of knee osteoarthritis in mice through primary cilia-mediated TRPV4 channels. Apoptosis : an international journal on programmed cell death. 2024-03-22. PMID:38517601. |
in this project, with the aim of revealing the mechanism of action of lipus, we proposed to use oa chondrocytes and animal models, lipus intervention, inhibition of primary cilia, use trpv4 inhibitors or trpv4 agonist, and use immunofluorescence (if), immunohistochemistry (ihc), western blot (wb), quantitative real-time pcr (qp) to detect the expression of cartilage synthetic matrix and endoplasmic reticulum stress markers. the results revealed that lipus altered primary cilia expression, promoted synthetic matrix metabolism in articular chondrocytes and was associated with primary cilia. |
2024-03-22 |
2024-03-25 |
mouse |
| Xiuchan Liu, Geng Zhang, Lei Liu, Guangyi Xiong, Jun Liu, Wei We. USP2 Promotes the Proliferation and Inflammation of Fibroblast-Like Synovial Cells in Rheumatoid Arthritis Through Deubiquitination of TRAF2. Biochemical genetics. 2024-03-14. PMID:38480669. |
protein expression was quantified using western blot (wb) analysis, while the quantities of inflammatory factors and matrix metalloproteinases were assessed using an elisa test. |
2024-03-14 |
2024-03-16 |
human |
| Yiming Li, Lingyun Zhao, Shiyi Li, Danhua Ruan, Lidan Xiong, Jie Tang, Meng Hu, Yixin Wang, Wen Huang, Li Li, Zhiwei Zha. Skin-derived precursor conditioned medium alleviated photoaging via early activation of TGF-β/Smad signaling pathway by thrombospondin1: In vitro and in vivo studies. Journal of photochemistry and photobiology. B, Biology. vol 253. 2024-02-27. PMID:38412778. |
consistent with the in vitro results, both western blot and immunohistochemistry results confirmed that protein expression of tsp1, smad2/3, p-smad2/3, tgf-β1, and collagen i increased, and matrix metalloproteinases decreased. |
2024-02-27 |
2024-03-01 |
mouse |
| Zhan Fang, Dan Wang, Fang Sun, Jing Chang, Dong Yuan, Shuhua Lin, Jian Ten. Circ-Luc7l Absence Attenuates Diabetic Nephropathy Progression by Reducing Mesangial Cell Excessive Proliferation, Inflammation, and Extracellular Matrix Accumulation via Mediating the miR-205-5p/Tgfbr1 Pathway. Biochemical genetics. 2024-02-20. PMID:38376578. |
the expression of extracellular matrix (ecm)-related markers and tgrbr1 protein was measured by western blot. |
2024-02-20 |
2024-02-22 |
mouse |
| Jie Li, Junqin Zhang, Zhaoping Chu, Hua Han, Yuan Zhan. FZD3 regulates the viability, apoptosis, and extracellular matrix degradation of vaginal wall fibroblasts in pelvic organ prolapse via the Wnt signaling pathway. Journal of biochemical and molecular toxicology. vol 38. issue 2. 2024-02-13. PMID:38348712. |
extracellular matrix (ecm) degradation was assessed by western blot analysis. |
2024-02-13 |
2024-02-15 |
Not clear |
| Gennaro Altamura, Manuela Martano, Anna Matrone, Annunziata Corteggio, Giuseppe Borzacchiell. Monoclonal antibody cetuximab impairs matrix metalloproteinases 2 and 9, epithelial-mesenchymal transition and cell migration in feline oral squamous cell carcinoma in vitro. Veterinary and comparative oncology. vol 22. issue 1. 2024-02-12. PMID:38030131. |
to this purpose, foscc cell lines sccf1, sccf2 and sccf3 were treated with cetuximab for 48/72 h and subjected to western blot for matrix metalloproteinases-2/9 (mmp-2/9) and epithelial-mesenchymal transition markers vimentin, e-, p- and n-cadherin. |
2024-02-12 |
2024-02-14 |
human |
| Manlin Zhu, Jingjing Lu, Xiaomin Li, Yongqiang An, Bohan Li, Wei Li. Ivabradine Alleviates Experimental Autoimmune Myocarditis-Mediated Myocardial Injury. International heart journal. vol 65. issue 1. 2024-02-06. PMID:38296563. |
western blot was employed to detect the expression of hcn1-4, mink-related protein 1 (mirp1), matrix metalloproteinase 2 (mmp-2), mmp-9, and transforming growth factor-β (tgf-β). |
2024-02-06 |
2024-02-09 |
rat |
| Xiaowei Qin, Jinjie Zhang, Mengmeng Wang, Yafei Feng, Yanbo Zhao, Huipeng Zhang, Wei Gu. The effect of thalidomide on the invasive ability of gastric cancer cells by regulating miR-524-5p/FSTL1. Cellular and molecular biology (Noisy-le-Grand, France). vol 69. issue 14. 2024-01-27. PMID:38279459. |
the experiment was divided into a blank control group, thal group (25 μg/ml thal-treated cells), thal+inhibitor nc group, and thal+mir-524-5p inhibitor group (transfected with inhibitor nc and mir-524-5p inhibitor respectively on the basis of thal group), cultivated for 48 h. the level of mir-524-5p in the cells was detected by rt-qpcr; the cell invasion was detected by transwell; the expression of matrix metalloproteinase (mmp)-2, mmp-9, fstl1 protein in the cells was detected by western blot. |
2024-01-27 |
2024-01-29 |
Not clear |
| Haixia Ming, Ming Shen, Yanwen Chen, Yang Li, Lingling Yang, Qiankun Liang, Zhaohua Li. [Establishment and evaluation of pre-metastatic niche mouse model labelled with luciferase in lewis lung cancer cells]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 39. issue 12. 2023-12-23. PMID:38140867. |
real-time quantitative pcr and western blot analysis were used to detect the mrna and protein expression of lysyl oxidase (lox), matrix metalloproteinase 9 (mmp9), versican (vcan), and fibronectin (fn), which are the specific markers for the formation of the microenvironment of lung tissues before metastasis. |
2023-12-23 |
2023-12-25 |
mouse |
| Jie Mei, Jing Zuo, Jiazhuan Mei, Guiju Liu, Peng Xia. Circ-NUP98 Promotes Lung Adenocarcinoma Development Through Regulating CBX1 by miR-188-3p. Biochemical genetics. 2023-12-22. PMID:38129720. |
cyclind1, bcl-2-related x protein (bax), matrix metalloproteinase 9 (mmp9) protein, and cbx1 protein levels were determined using western blot. |
2023-12-22 |
2023-12-24 |
human |
| Mengzhe Zhang, Fenfen Xiang, Yipeng Sun, Rongrong Liu, Qian Li, Qing Gu, Xiangdong Kang, Rong W. Ursolic acid inhibits the metastasis of colon cancer by downregulating ARL4C expression. Oncology reports. vol 51. issue 2. 2023-12-22. PMID:38131251. |
the expression levels of proteins including arl4c, matrix metallopeptidase 2 (mmp2), phosphorylated (p)‑akt and p‑mtor were measured using western blot analysis. |
2023-12-22 |
2023-12-24 |
mouse |
| Xin-Yi Fan, Xiao-Qi Wei, Yun-Yang Zhang, Hai-Yin Pu, Fang-He Li, Kuo Gao, Xue Yu, Shu-Zhen Gu. [Effect and mechanism of Jiming Powder on myocardial fibrosis in mice with myocardial infarction]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 21. 2023-12-19. PMID:38114180. |
western blot analysis was performed to detect key proteins involved in ventricular remodeling, including transforming growth factor-β1(tgf-β1), α-smooth muscle actin(α-sma), wingless-type mmtv integration site family member 3a(wnt3a), β-catenin, matrix metallopeptidase 2(mmp2), matrix metallopeptidase 3(mmp3), timp metallopeptidase inhibitor 1(timp1), and timp metallopeptidase inhibitor 2(timp2). |
2023-12-19 |
2023-12-23 |
mouse |
| Xin-Yi Fan, Xiao-Qi Wei, Yun-Yang Zhang, Hai-Yin Pu, Fang-He Li, Kuo Gao, Xue Yu, Shu-Zhen Gu. [Effect and mechanism of Jiming Powder on myocardial fibrosis in mice with myocardial infarction]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 21. 2023-12-19. PMID:38114180. |
western blot results showed that jiming powder reduced the expression of tgf-β1, α-sma, wnt3a, and β-catenin, decreased the levels of mmp2, mmp3, and timp2, and increased the level of timp1, suggesting its role in inhibiting cardiac fibroblast transformation, reducing extracellular matrix metabolism in myocardial cells, and lowering collagen Ⅰ and α-sma content, thus exerting an anti-myocardial fibrosis effect after myocardial infarction. |
2023-12-19 |
2023-12-23 |
mouse |
| Zhifang Tang, Jingyuan Li, Chuan L. Post-Transcriptional Regulator RBM47 Stabilizes FBXO2 mRNA to Advance Osteoarthritis Development: WGCNA Analysis and Experimental Validation. Biochemical genetics. 2023-12-09. PMID:38070024. |
functional analysis results from the mtt assay, flow cytometry, evaluation of ldh activities and inflammatory mediators, and western blot analysis of extracellular matrix (ecm) proteins, showed that rbm47 knockdown significantly alleviated inflammation, apoptosis, and ecm degradation in interleukin 1β (il-1β)-treated chondrocytes. |
2023-12-09 |
2023-12-17 |
Not clear |
| Ying-Jie Zhu, Hu-Lin Chen, Xin-Jie Cai, Bang-le Zhan, Xiao-Ming Li. Naringin acts as a TRPV1 antagonist to attenuate UVB-induced senescence and damage in HaCaT cells. Chemical biology & drug design. 2023-11-09. PMID:37945519. |
analysis was performed using the mtt assay to assess cell viability, flow cytometry to measure the apoptosis level, sa-β-gal staining to observe cellular senescence, and western blot to assess protein levels of trpv1, p16, p53, p21, matrix metalloproteinase (mmp)-1, and mmp-9. |
2023-11-09 |
2023-11-20 |
human |
| Zhe Ma, Yufei Cai, Chenchen Tia. ZWINT promotes the proliferation, migration, and invasion of cervical cancer cells by regulating the p53/p21 signaling pathway. The Chinese journal of physiology. vol 66. issue 5. 2023-11-06. PMID:37929349. |
moreover, western blot was used to measure the expression level of zwint, matrix metalloproteinase 9 (mmp-9), n-cadherin, e-cadherin, p53 and p21 in caski and hela cells with zwint overexpression or knockdown. |
2023-11-06 |
2023-11-08 |
Not clear |
| Cheng Teng, Jingtao Wu, Zhao Zhang, Jinquan Wang, Ye Yang, Chengji Dong, Long Wu, Zhen Lin, Yuezheng Hu, Jing Wang, Xiaolei Zhang, Zhongke Li. Fucoxanthin ameliorates endoplasmic reticulum stress and inhibits apoptosis and alleviates intervertebral disc degeneration in rats by upregulating Sirt1. Phytotherapy research : PTR. 2023-11-02. PMID:37918392. |
qrt-pcr, western blot, immunofluorescence, and tunel staining were used to detect apoptosis, ers, extracellular matrix (ecm), and sirt1-related pathways. |
2023-11-02 |
2023-11-08 |
rat |