All Relations between blot and matrix compartment

Publication Sentence Publish Date Extraction Date Species
Ying-Jie Zhu, Hu-Lin Chen, Xin-Jie Cai, Bang-le Zhan, Xiao-Ming Li. Naringin acts as a TRPV1 antagonist to attenuate UVB-induced senescence and damage in HaCaT cells. Chemical biology & drug design. 2023-11-09. PMID:37945519. analysis was performed using the mtt assay to assess cell viability, flow cytometry to measure the apoptosis level, sa-\xce\xb2-gal staining to observe cellular senescence, and western blot to assess protein levels of trpv1, p16, p53, p21, matrix metalloproteinase (mmp)-1, and mmp-9. 2023-11-09 2023-11-20 human
Zhe Ma, Yufei Cai, Chenchen Tia. ZWINT promotes the proliferation, migration, and invasion of cervical cancer cells by regulating the p53/p21 signaling pathway. The Chinese journal of physiology. vol 66. issue 5. 2023-11-06. PMID:37929349. moreover, western blot was used to measure the expression level of zwint, matrix metalloproteinase 9 (mmp-9), n-cadherin, e-cadherin, p53 and p21 in caski and hela cells with zwint overexpression or knockdown. 2023-11-06 2023-11-08 Not clear
Cheng Teng, Jingtao Wu, Zhao Zhang, Jinquan Wang, Ye Yang, Chengji Dong, Long Wu, Zhen Lin, Yuezheng Hu, Jing Wang, Xiaolei Zhang, Zhongke Li. Fucoxanthin ameliorates endoplasmic reticulum stress and inhibits apoptosis and alleviates intervertebral disc degeneration in rats by upregulating Sirt1. Phytotherapy research : PTR. 2023-11-02. PMID:37918392. qrt-pcr, western blot, immunofluorescence, and tunel staining were used to detect apoptosis, ers, extracellular matrix (ecm), and sirt1-related pathways. 2023-11-02 2023-11-08 rat
Jussi M Leppilahti, Taina Tervahartiala, Hannu Kautiainen, Ismo R\\xc3\\xa4is\\xc3\\xa4nen, Minna-Maija Ahonen, Veli-Jukka Uitto, Timo Sorsa, P\\xc3\\xa4ivi M\\xc3\\xa4ntyl\\xc3\\xa. Correlations between different protein species of oral rinse MMP-8 and patient-related factors. Clinical and experimental dental research. 2023-10-25. PMID:37877535. the aim of this study is to examine correlations between different oral rinse matrix metalloproteinase (mmp)-8 protein species in western blot (wb) analysis, quantitative mmp-8 measurements, and patient-related factors. 2023-10-25 2023-11-08 Not clear
Yueh-Chung Chen, Chen-Chung Liao, Hao-Ai Shui, Pei-Hsuan Huang, Li-Jane Shi. A Proteomics-Based Identification of the Biological Networks Mediating the Impact of Epigallocatechin-3-Gallate on Trophoblast Cell Migration and Invasion, with Potential Implications for Maternal and Fetal Health. Proteomes. vol 11. issue 4. 2023-10-24. PMID:37873873. the quantitative pcr showed that egcg modulated protein expression by regulating gene transcription, and the western blot analysis confirmed its impact on cytoskeleton and extracellular matrix reorganization. 2023-10-24 2023-11-08 Not clear
Weiyi Xia, Jian Xiao, ChengLin Tong, Jiajie Lu, Yurong Tu, Sunlong Li, Libing Ni, Yifeng Shi, Peng Luo, Xiaolei Zhang, Xiangyang Wan. Orientin inhibits inflammation in chondrocytes and attenuates osteoarthritis through Nrf2/NF-\\xce\\xbaB and SIRT6/NF-\\xce\\xbaB pathway. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. vol 41. issue 11. 2023-10-09. PMID:37186383. in this study, a significant suppression in il-1\xce\xb2-mediated pro-inflammatory mediators and the degradation of cartilage extracellular matrix (ecm) was observed in vitro through qpcr, western blot analysis, elisa, and immunofluorescent staining after the treatment with orientin. 2023-10-09 2023-10-15 mouse
Xiao-Hui Meng, Sheng Zhong, Hai-Hui Han, Qi Shi, Song-Tao Sun, Lian-Bo Xia. [Effect of Juanbi Qianggu Formula on biological behaviors of fibroblast-like synoviocytes in rheumatoid arthritis by regulating FGFR1 signaling pathway based on network pharmacology and cell function experiments]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 18. 2023-10-06. PMID:37802828. the biological effect of jbqgf by inhibiting fgfr1 phosphorylation was preliminarily verified by western blot, transwell invasion assay, and pannus erosion assay, thereby inhibiting matrix metalloproteinase 2(mmp2) and receptor activator of nuclear factor-\xce\xbab ligand(rankl) and suppressing the invasion of fibroblasts in rheumatoid arthritis and erosive effect of pannus bone. 2023-10-06 2023-10-15 rat
Ying-Ying Kang, Qian Qian, Ya Yang, Ying Yang, Fang Xu, Min Li, Jian-Guang L. [Effect of acetylalkannin from Arnebia euchroma on proliferation, migration, and invasion of human melanoma A375 cells]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 18. 2023-10-06. PMID:37802847. western blot was used to detect the protein expression of migration and invasion-related n-cadherin, vimentin, matrix metalloproteina-se-9(mmp-9), and wnt/\xce\xb2-catenin pathway-related wnt1, axin2, glycogen synthase kinase-3\xce\xb2(gsk-3\xce\xb2), phosphorylated gsk-3\xce\xb2(p-gsk-3\xce\xb2), \xce\xb2-catenin, cell cycle protein d_1(cyclin d_1), and p21. 2023-10-06 2023-10-15 human
Hong-Ling Ou, Hui Wu, Yu-Liang Ren, Yuan Si, Zhong-Qi Duan, Xue-Wen Li. [Hydnocarpin inhibits malignant progression of triple negative breast cancer via CNOT4-mediated ubiquitination and degradation of YAP]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 16. 2023-10-06. PMID:37802875. the changes in the epithelial-mesenchymal transition(emt) and the expression of invasion-and migration-associated proteins [e-cadherin, vimentin, snail, matrix metalloproteinase-2(mmp-2), and mmp-9] were detected by western blot. 2023-10-06 2023-10-15 Not clear
Gang Che. Molecular basis of breast cancer with comorbid depression and the mechanistic insights of Xiaoyaosan in treating breast cancer-associated depression. Medicine. vol 102. issue 38. 2023-09-25. PMID:37747031. the impact of xys on the gene and protein expression of matrix metallopeptidase 9 (mmp9) in microglial cells was assessed using rt-quantitative pcr and western blot analysis, respectively. 2023-09-25 2023-10-07 Not clear
Sezgin Zeren, Semih Seker, Gizem Akka\\xc5\\x9f Akg\\xc3\\xbcn, Emrah Okur, Azmi Yerlikay. Label-free nLC-MS/MS proteomic analysis reveals significant differences in the proteome between colorectal cancer tissues and normal colon mucosa. Medical oncology (Northwood, London, England). vol 40. issue 10. 2023-09-14. PMID:37707637. western blot studies confirmed that rbmx protein (critical for gene transcription and alternative splicing of various pre-mrnas) was increased 2.04-fold, while decorin protein (a matrix proteoglycan with tumor suppressor functions) was dramatically decreased by about 6.04-fold in tumor samples compared with normal mucosa. 2023-09-14 2023-10-07 Not clear
Kanna Xu, Haoran Wang, Zhongqing W. Genkwanin suppresses mitochondrial dysfunction to alleviate IL-1\\xce\\xb2-elicited inflammation, apoptosis, and degradation of extracellular matrix in chondrocytes through upregulating DUSP1. The Chinese journal of physiology. vol 66. issue 4. 2023-08-28. PMID:37635488. in addition, immunofluorescence staining and western blot were utilized to measure the expression of extracellular matrix (ecm)-associated proteins. 2023-08-28 2023-09-07 Not clear
Sadia Afrin, Gregory W Kirschen, Mostafa A Boraha. Obesity Contributes to Transformation of Myometrial Stem-Cell Niche to Leiomyoma via Inducing Oxidative Stress, DNA Damage, Proliferation, and Extracellular Matrix Deposition. Genes. vol 14. issue 8. 2023-08-26. PMID:37628676. enzyme-linked immunosorbent assay (elisa), western blot, and immunocytochemistry assays were performed on human adipocytes (sw872) co-cultured with mscs and treated with leptin or adiponectin to examine the effects of proliferation, extracellular matrix (ecm) deposition, oxidative damage, and dna damage. 2023-08-26 2023-09-07 human
Ioanna Papathanasiou, Charalampos Balis, Dimitrios Destounis, Evanthia Mourmoura, Aspasia Tsezo. NEAT1-mediated miR-150-5p downregulation regulates b-catenin expression in OA chondrocytes. Functional & integrative genomics. vol 23. issue 3. 2023-07-19. PMID:37468759. mir-150-5p, neat1, b-catenin, matrix metallopeptidase 13 (mmp-13), and adam metallopeptidase with thrombospondin type 1 motif 5 (adamts-5) expressions were assessed by reverse transcription-quantitative pcr (rt-qpcr) and western blot assay. 2023-07-19 2023-08-14 Not clear
Shipra Kanti Jena, Shreya Das, Shreeta Chakraborty, Rupasri Ai. Molecular determinants of epithelial mesenchymal transition in mouse placenta and trophoblast stem cell. Scientific reports. vol 13. issue 1. 2023-07-06. PMID:37414855. further validation of array results using real time pcr and western blot analysis showed significant decrease in emt-associated genes that included (a) transcription factors (snai2, zeb1, stat3 and foxc2), (b) extracellular matrix and cell adhesion related genes (bmp1, itga5, vcan and col3a1), (c) migration and motility- associated genes (vim, msn and fn1) and (d) differentiation and development related genes (wnt5b, jag1 and cleaved notch-1) on e9.5. 2023-07-06 2023-08-14 mouse
Alaa M Altaie, Mohammad G Mohammad, Mohamed I Madkour, Mohammed Amjed AlSaegh, Manju Nidagodu Jayakumar, Aghila Rani K G, A R Samsudin, Rabih Halwani, Rifat A Hamoudi, Sameh S M Solima. Molecular pathogenicity of 1-nonadecene and L-lactic acid, unique metabolites in radicular cysts and periapical granulomas. Scientific reports. vol 13. issue 1. 2023-07-03. PMID:37400519. the collagen, matrix metalloproteinase (mmp)-1, and released cytokines were measured using collagen assay, western blot, and luminex assay, respectively. 2023-07-03 2023-08-14 Not clear
Xiaofei Ren, Kui Xu, Jianming Xu, Qiao Me. Melatonin attenuates monocrotaline-induced hepatic sinusoidal obstruction syndrome in rats via activation of Sirtuin-3. Journal of biochemical and molecular toxicology. 2023-06-23. PMID:37350538. real-time polymerase chain reaction and western blot analysis were used to identify\xc2\xa0liver sirtuin-3 (sirt3) and active matrix metallopeptidase 9 (mmp-9) expression. 2023-06-23 2023-08-14 rat
Xiu-Fan Du, Kai Huang, Xiao-Yan Chen, Chun-Hang Huang, Hui-Yuan Cao, Guang-Ji Wang, Yong H. Gremlin-1 promotes IL-1\\xce\\xb2-stimulated chondrocyte inflammation and extracellular matrix degradation via activation of the MAPK signaling pathway. Journal of biochemical and molecular toxicology. 2023-06-23. PMID:37352019. besides, the influence of knocking down/over-expressing gremlin-1 on the inflammatory factors (il-6, tnf-\xce\xb1, il-18 and pge2), oxidative stress-related substances (malondialdehyde [mda]; superoxide dismutase [sod]; lactate dehydrogenase [ldh]), extracellular matrix (ecm) degradation-related proteins, and mitogen-activated protein kinase (mapk) pathway proteins in il-1\xce\xb2-stimulated chon-001 cells were tested by enzyme-linked immunosorbent assay, related kits, qrt-pcr, and western blot, respectively. 2023-06-23 2023-08-14 human
Xuefeng Zhang, Zhikun Wang, Binbin Wang, Jingyi Li, Hui Yua. lncRNA OIP5-AS1 attenuates the osteoarthritis progression in IL-1\\xce\\xb2-stimulated chondrocytes. Open medicine (Warsaw, Poland). vol 18. issue 1. 2023-06-19. PMID:37333451. after the expression of oip5-as1 or mir-338-3p in interleukin (il)-1\xce\xb2-stimulated primary chondrocytes and chon-001 cells was manipulated, cell viability, proliferation, apoptosis rate, apoptosis-related protein (cleaved caspase-9, bax) expressions, extracellular matrix (ecm) (matrix metalloproteinase (mmp)-3, mmp-13, aggrecan, and collagen ii), pi3k/akt pathway, and mrna expressions of inflammatory factors (il-6 and il-8), oip5-as1, and mir-338-3p were determined by cell counting kit-8, edu, flow cytometry, western blot, and quantitative reverse transcription-polymerase chain reaction. 2023-06-19 2023-08-14 Not clear
Ying Yang, Ok-Su Kim, Guo Liu, Bin-Na Lee, Danyang Liu, Wenqi Fu, Siyu Zhu, Jae-Seok Kang, Byunggook Kim, Okjoon Ki. Effects of Red LED Irradiation in Enhancing the Mineralization of Human Dental Pulp Cells In Vitro. International journal of molecular sciences. vol 24. issue 11. 2023-06-11. PMID:37298716. western blot also indicated that red ledi treatment in the mineralization stage, but not the proliferation or differentiation stages, upregulated the expression of dentin matrix marker proteins (dentin sialophosphoprotein, dspp; dentin matrix protein 1, dmp1; osteopontin, opn) and an intracellular secretory vesicle marker protein (lysosomal-associated membrane protein 1, lamp1). 2023-06-11 2023-08-14 human