| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Xiang Xu, LiangChao He, MingMing Li, YongHao Zhang, Qian-Qian Li, ShiYong Fang, Guang Che. Neuroinflammation Plays a Potential Role in the Medulla Oblongata after Moderate Traumatic Brain Injury in Mice as Revealed by Nontargeted Metabonomics Analysis. Journal of neurotrauma. 2024-05-02. PMID:38695184. |
in addition, we confirmed increases in the levels of inflammatory factors and the activation of astrocytes and microglia by western blot and immunofluorescence staining, and these findings were consistent with the nontargeted metabolomics results. |
2024-05-02 |
2024-05-04 |
mouse |
| Bin Fan, Junbin Lin, Qihang Luo, Weijing Liao, Chizi Ha. Enriched Environment Inhibits Neurotoxic Reactive Astrocytes via JAK2-STAT3 to Promote Glutamatergic Synaptogenesis and Cognitive Improvement in Chronic Cerebral Hypoperfusion Rats. Neurotoxicity research. vol 42. issue 2. 2024-04-02. PMID:38564082. |
the protein levels of glutamatergic synapses, neurotoxic reactive astrocytes, reactive microglia, and jak2-stat3 signaling pathway were measured using western blot. |
2024-04-02 |
2024-04-04 |
rat |
| Sharif Ahmed, Ning Ma, Jun Kawanokuchi, Keiya Matsuoka, Shinji Oikawa, Hatasu Kobayashi, Yusuke Hiraku, Mariko Murat. Taurine reduces microglia activation in the brain of aged senescence-accelerated mice by increasing the level of TREM2. Scientific reports. vol 14. issue 1. 2024-03-29. PMID:38548872. |
immunohistochemical and western blot analyses revealed that taurine significantly reduced the number of activated microglia, levels of phospho-tau and aβ deposit in the hippocampus and cortex. |
2024-03-29 |
2024-03-31 |
mouse |
| Irena Majkutewicz, Ewelina Kurowska-Rucińska, Jan Ruciński, Dorota Myślińska, Beata Grembecka, Jagoda Mantej, Katarzyna P Dzi. Diverse Efficacy of Dimethyl Fumarate in Alleviating the Late Streptozotocin-Induced Cognitive Impairment and Neuropathological Features in Rat. Molecular neurobiology. 2024-03-02. PMID:38430351. |
spatial memory was assessed with morris water maze (mwm), general brain level of amyloid β (aβ) and p-tau was measured by western blot, immunofluorescent labelling of active microglia (iba1), aβ and p-tau and histological assay of neurodegeneration (fluoro-jade c) were performed in hippocampus and cortex. |
2024-03-02 |
2024-03-04 |
human |
| Gangjing Li, Li Hu, Xiangcheng Gu, Weijun Zhu, Xuechu Zhen, Xiaohui Su. Targeting Large-Conductance Calcium-Activated Potassium Channels to Ameliorate Lipopolysaccharide-Induced Depressive-Like Behavior in Mice. Neurochemical research. 2024-02-22. PMID:38383879. |
the morphology of microglia, expression of bk channels, levels of cytokines, and expression and activity of indoleamine 2,3-dioxygenase (ido) were measured by immunohistochemistry, western blot, quantitative real time pcr, and enzyme-linked immunosorbent assay (elisa), respectively. |
2024-02-22 |
2024-02-24 |
mouse |
| Bingxin Zhao, Sheng Zhang, Nashwa Amin, Jie Pan, Fei Wu, Guanghong Shen, Mingming Tan, Zongjie Shi, Yu Gen. Thymoquinone regulates microglial M1/M2 polarization after cerebral ischemia-reperfusion injury via the TLR4 signaling pathway. Neurotoxicology. 2024-02-07. PMID:38325603. |
secondly, real-time fluorescence quantitative pcr, immuno-fluorescence, elisa, and western blot were used to detect the expression of m1/ m2-specific markers in microglia to explore the role of tq in the modulation of microglial cell polarization after cerebral ischemia-reperfusion. |
2024-02-07 |
2024-02-10 |
mouse |
| Hui Guo, Li-Qing Chen, Zhi-Ru Zou, Shuai Cheng, Yu Hu, Liang Mao, He Tian, Xi-Fan Me. Zinc remodels mitochondrial network through SIRT3/Mfn2-dependent mitochondrial transfer in ameliorating spinal cord injury. European journal of pharmacology. 2024-02-05. PMID:38316246. |
in the study, we used transwell culture, western blot, quantitative real-time polymerase chain reaction (qrt-pcr), atp content detection, reactive oxygen species (ros) activity assay, flow cytometry and immunostaining to investigate the relationship between zinc-treated microglia and injured neurons through animal and cell experiments. |
2024-02-05 |
2024-02-09 |
mouse |
| Lisheng Wang, Zhonghua Cai, Qi Gu, Changli X. cGAS Deficiency Regulates the Phenotypic Polarization and Glycolysis of Microglia Through Lactylation in Hypoxic-Ischemic Encephalopathy Cell Model. Biochemical genetics. 2024-01-21. PMID:38246965. |
moreover, the lactylation of cgas in ogd treated microglia was evaluated by western blot. |
2024-01-21 |
2024-01-24 |
Not clear |
| Fang-Ting Zhou, Ying Zong, Wu-Qiong Hou, Sen-Sen Li, Fei Yang, Li-Ting Xu, Xia Mao, Yu-Dong Liu, Xiao-Hui Su, Hong-Ye Wan, Jing-Feng Ouyang, Qiu-Yan Guo, Wei-Jie Li, Zhen Wang, Chao Wang, Na Li. [Baimai Ointment relieves chronic pain induced by chronic compression of dorsal root ganglion in rats by regulating neuroactive ligand-receptor interaction and HIF-1 signaling pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 23. 2024-01-11. PMID:38212003. |
furthermore, behavioral tests, western blot, and immunofluorescence assay revealed that the pain-relieving effect of baimai ointment on ccd may be related to the regulation of the interaction between neuroactive ligand and receptors(neuroligands) such as chrna7, adra2a, and adrb2, and the down-regulation of the expression of nos2/perk/pi3k, the core regulatory element of hif-1 signaling pathway in spinal microglia. |
2024-01-11 |
2024-01-14 |
mouse |
| Yongri Hong, Caichuan Wei, Miaoying Fu, Xinyang Li, Haiju Zhang, Baozhen Ya. MCC950 alleviates seizure severity and angiogenesis by inhibiting NLRP3/ IL-1β signaling pathway-mediated pyroptosis in mouse model of epilepsy. International immunopharmacology. vol 126. 2023-12-01. PMID:38039716. |
in addition, immunofluorescence staining, rt-qpcr, and western blot demonstrated that mcc950 inhibited the expression of the nlrp3 inflammasome and its related proteins in microglia, whereas microangiogenesis was found to be increased in the cerebral cortex and hippocampus of epileptic mice, and these effects could be reversed by mcc950. |
2023-12-01 |
2023-12-10 |
mouse |
| Xue Yang, Jianjiang Wu, Hu Cheng, Siyu Chen, Jiang Wan. DEXMEDETOMIDINE AMELIORATES ACUTE BRAIN INJURY INDUCED BY MYOCARDIAL ISCHEMIA-REPERFUSION VIA UPREGULATING THE HIF-1 PATHWAY. Shock (Augusta, Ga.). vol 60. issue 5. 2023-11-03. PMID:37647083. |
the impact of dex on brain injury elicited by myocardial ir was assessed via elisa for inflammatory factors in serum and brain; evans blue for blood-brain barrier permeability; hematoxylin-eosin staining for pathological injury in brain; immunofluorescence for microglia activation in brain; morris water maze for cognitive dysfunction; western blot for the expression level of hif-1α, occludin, cleaved caspase-3, nf-κb p65, and p-nf-κb p65 in the brain. |
2023-11-03 |
2023-11-08 |
mouse |
| Timna Hitrec, Fabio Squarcio, Emiliana Piscitiello, Matteo Cerri, Davide Martelli, Alessandra Occhinegro, Ludovico Taddei, Domenico Tupone, Roberto Amici, Marco Lupp. Sleep deprivation soon after recovery from synthetic torpor enhances tau protein dephosphorylation in the rat brain. Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology. 2023-10-09. PMID:37812305. |
the effects of sd were studied by assessing: (i) deep brain temperature (tb); (ii) immunofluorescent staining for at8 (phosphorylated tau) and tau-1 (non-phosphorylated tau), assessed in 19 brain structures; (iii) different phosphorylated forms of tau and the main cellular factors involved in tau phospho-regulation, including pro- and anti-apoptotic markers, assessed through western blot in the parietal cortex and hippocampus; (iv) systemic factors which are involved in natural torpor; (v) microglia activation state, by considering morphometric variations. |
2023-10-09 |
2023-10-15 |
rat |
| Fang-Ting Zhou, Ying Zong, Yuan-Bin Li, Ren-Li Cao, Wu-Qiong Hou, Li-Ting Xu, Fei Yang, Yan-Li Gu, Xiao-Hui Su, Qiu-Yan Guo, Wei-Jie Li, Hui Xiong, Chao Wang, Na Li. [Mechanism of Mongolian drug Naru-3 in initiation of neuroinflammation of neuropathic pain from MMP9/IL-1β signaling pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 15. 2023-10-06. PMID:37802786. |
by means of western blot, immunofluorescence, agonists, antagonists, behavior, etc., the mechanism of naru-3 in exerting np analgesia may be related to the negative regulation of the mmp9/il-1β signaling pathway-mediated microglia p38/il-1β inflammatory loop in the activation phase. |
2023-10-06 |
2023-10-15 |
mouse |
| Jinhan Wu, Shiqi Mao, Xiang Wu, Yi Zhao, Weijun Zhang, Feng Zh. Jasminoidin reduces ischemic stroke injury by regulating microglia polarization via PASK-EEF1A1 axis. Chemical biology & drug design. 2023-09-24. PMID:37743322. |
the mrna or protein expressions of examined genes in microglia and brain tissues were detected by quantitative real-time polymerase chain reaction or western blot. |
2023-09-24 |
2023-10-07 |
mouse |
| Vaibhave Ubba, Serene Joseph, Olubusayo Awe, Dustin Jones, Milan K Dsilva, Mingxiao Feng, Junjiang Wang, Xiaomin Fu, Razeen J Akbar, Brittany H Bodnar, Wenhui Hu, Hong Wang, Xiaofeng Yang, Ling Yang, Peixin Yang, Rexford Ahima, Sara Divall, Sheng W. Neuronal AR regulates glucose homeostasis and energy expenditure in lean female mice with androgen excess. Endocrinology. 2023-09-22. PMID:37738624. |
western blot studies show that markers of inflammation and microglia activation, such as nf-kb p-65 and iba1, increased in the hypothalamus of con-dht mice compared to con-veh. |
2023-09-22 |
2023-10-07 |
mouse |
| Xue Xia, Jiahao Chen, Haiyuan Ren, Chang Zhou, Qingli Zhang, Haoyang Cheng, Xiaojing Wan. Gypenoside Pretreatment Alleviates the Cerebral Ischemia Injury via Inhibiting the Microglia-Mediated Neuroinflammation. Molecular neurobiology. 2023-09-09. PMID:37688709. |
in this study, we investigated whether gps could reduce neuroinflammation by regulating microglia and the underlying mechanism through qrt-pcr and western blot. |
2023-09-09 |
2023-10-07 |
mouse |
| Lei Li, Guojun He, Mingyu Shi, Juehua Zhu, Yongqing Cheng, Yang Chen, Jin Chen, Qun Xu. Edaravone dexborneol ameliorates cognitive impairment by regulating the NF-κB pathway through AHR and promoting microglial polarization towards the M2 phenotype in mice with bilateral carotid artery stenosis (BCAS). European journal of pharmacology. 2023-09-06. PMID:37673366. |
western blot analysis demonstrated that m1 microglia dominated prophase and released proinflammatory molecules; the aryl hydrocarbon receptor (ahr) was found to participate in modulating nuclear factor-kappa b (nf-κb) signalling activation through tumour necrosis factor receptor-associated factor-6 (traf6). |
2023-09-06 |
2023-10-07 |
mouse |
| Xiaoli Chen, Yalei Ning, Bo Wang, Jun Qin, Changhong Li, Ruobing Gao, Zhihui Ma, Yuanguo Zhou, Ping Li, Yan Zhao, Yan Peng, Xing Chen, Nan Yang, Shiyu Sh. HET0016 inhibits neuronal pyroptosis in the immature brain post-TBI via the p38 MAPK signaling pathway. Neuropharmacology. 2023-08-14. PMID:37579871. |
western blot analysis revealed reduced expression of p-p38 mapk and nuclear factor-kappa b (nf-κb) p65 in the neurons and microglia upon het0016 treatment in tbi rats. |
2023-08-14 |
2023-09-07 |
rat |
| Lushu Chen, Yuan Cao, Yaming Shen, Huan Li, Rong Ye, Jin Ya. Downregulation of PIK3IP1 in retinal microglia promotes retinal pathological neovascularization via PI3K-AKT pathway activation. Scientific reports. vol 13. issue 1. 2023-08-07. PMID:37550343. |
then we used pcr, western blot and elisa techniques to confirm that the expression of vegfa, fgf2, hgfα and mmp9 was increased in microglia after lipopolysaccharide (lps) induction. |
2023-08-07 |
2023-08-14 |
Not clear |
| Yousef Mohamadi, Maryam Borhani-Haghigh. TGN020 application against aquaporin 4 improved multiple sclerosis by inhibiting astrocytes, microglia, and NLRP3 inflammasome in a cuprizone mouse model. Journal of chemical neuroanatomy. 2023-07-02. PMID:37394105. |
astrogliosis, m1-m2 microglia polarization, nlrp3 inflammasome activation, and demyelination were investigated in the corpus callosum by immunohistochemistry, real-time pcr, western blot, and luxol fast blue staining. |
2023-07-02 |
2023-08-14 |
mouse |